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1、实验五实验五 利用利用 EntrezEntrez 、SRSSRS 工具检索权关核酸蛋白数据库工具检索权关核酸蛋白数据库1. Limits Activated: exclude patents, Source database: EMBL（1）. Hepatitis B virus complete genome, genotype A2, isolate P2.2.11GenBank: HE576989.1FASTA Graphics Go to: LOCUS HE576989 3221 bp DNA circular VRL 29-JUL-2011 DEFINITION Hepatitis
2、B virus complete genome, genotype A2, isolate P2.2.11. ACCESSION HE576989 VERSION HE576989.1 GI:341823528 KEYWORDS complete genome. SOURCE Hepatitis B virusORGANISM Hepatitis B virusViruses; Retro-transcribing viruses; Hepadnaviridae;Orthohepadnavirus. REFERENCE 1AUTHORS Martel,N., Cotte,L., Trabaud
3、,M.A., Trepo,C., Zoulim,F., Gomes,S.and Kay,A.TITLE Glucocorticoid-induced reactivation of an occult HBV infection inan HIV+ patientJOURNAL Unpublished REFERENCE 2 (bases 1 to 3221)AUTHORS Kay,A.C.TITLE Direct SubmissionJOURNAL Submitted (22-JUL-2011) to the INSDC. Kay A.C., U1052, INSERM, 151cours
4、Albert Thomas, Lyon, 69003, FRANCE FEATURES Location/Qualifierssource 1.3221/organism=“Hepatitis B virus“/mol_type=“genomic DNA“/serotype=“ayw1“/isolate=“P2.2.11“/db_xref=“taxon:10407“/country=“France“/collection_date=“09-Jun-2007“/note=“genotype:A2“gene order(2307.3221,1.1623)/gene=“pol“CDS join(23
5、07.3221,1.1623)/gene=“pol“/function=“viral polymerase“/codon_start=1/product=“reverse transcriptase-DNA polymerase“/protein_id=“CCC82739.1“/db_xref=“GI:341823532“/translation=“MPLSYQHFRKLLLLDDGTEAGPLEEELPRLADADLNRRVAE DLNLGNLNVSIPWTHKVGNFTGLYSSTVPIFNPDWQTPSFPKIHLQEDIINRCQQFVG PLTVNEKRRLKLIMPARFYPTHT
6、KYLPLDKGIKPYYPDQVVNHYFQTRHYLHTLWKAG ILYKRETTRSASFCGSPYSWEQELQHGRLVIKTSQRHGDESFCSQPSGILSRSSVGPC IRSQLKQSRLGLQPHQGPLASSQPGRSGSIRARAHPSTRRYFGVEPSGSGHIDHSVNN SSSCLHQSAVRKAAHSHLSTSKRQSSSGHAVESHCLPPSSAGSQSQGSVFSCWWLQFR NSKPCSEYCLSHLVNLREDWGPCDEHGEHHIRIPRTPARVTGGVFLVDKNPHNTTESR LVVDFSQFSRGITRVSWPKFAVPNLQSL
7、TNLLSSNLSWLSLDVSAAFYHIPLHPAAMP HLLIGSSGLSGYVARLSSNSRINNNQYGTMQNLHDSCSRQLYVSLMLLYKTYGRKLHL YSHPIVLGFRKIPMGVGLSPFLLAQFTSAICSVVRRAFPHCLAFSYMDDVVLGAKSVQ HRESLYTAVTNFLLSLGIHLNPNKTKRWGYSLNFMGYIIGSWGTLPQDHIVQKIKHCF RKLPVNRPIDWKVCQRIVGLLGFAAPFTQCGYPALMPLYACIQAKQAFTFSPTYKAFL SKQYMNLYPVARQRPGLCQVFADATPTGWGLAI
8、GHQRMRGTFVAPLPIHTAELLAACF ARSRSGAKLIGTDNSVVLSRKYTSFPWLLGCTANWILRGTSFVYVPSALNPADDPSRG RLGLSRPLLRLPFQPTTGRTSLYAVSPSVPSHLPVRVHFASPLHVAWRPP“gene order(2854.3221,1.835)/gene=“preS1“CDS join(2854.3221,1.835)/gene=“preS1“/function=“envelope protein“/codon_start=1/product=“large surface antigen“/protein_id=
9、“CCC82740.1“/db_xref=“GI:341823533“/translation=“MGGWSSKPRKGMGTNLSVPNPLGFFPDHQLDPAFGANSNNP DWDFNPIKDHWPAANQVGVGAFGPGLTPPHGGILGWSPQAQGILTTVSTIPPPASTNR QSGRQPTPISPPLRDSHPQAMQWNPTAFHQALQDPRVRGLYFPAGGSSSGTVNPAPNI ASHISSISARTGDPVTNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNF LGGSPVCLGQNSQSPTSNHSPTSCPPICPGYR
10、WMCLRRFIIFLFILLLCLIFLLVLLD YQGMLPVCPLIPGSTTTSTGPCRTCTTPAQGNSMFPSCCCTKPTEGNCTCIPIPLSWA FAKYLWEWASVRFSWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYSIVSPFIPL LPIFFCLWVYI“gene order(3211.3221,1.835)/gene=“preS2“CDS join(3211.3221,1.835)/gene=“preS2“/function=“envelope protein“/codon_start=1/product=“middle surfac
11、e antigen“/protein_id=“CCC82741.1“/db_xref=“GI:341823534“/translation=“MQWNPTAFHQALQDPRVRGLYFPAGGSSSGTVNPAPNIASH ISSISARTGDPVTNMENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNFLGG SPVCLGQNSQSPTSNHSPTSCPPICPGYRWMCLRRFIIFLFILLLCLIFLLVLLDYQG MLPVCPLIPGSTTTSTGPCRTCTTPAQGNSMFPSCCCTKPTEGNCTCIPIPLSWAFAK YLWEWASVRF
12、SWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYSIVSPFIPLLPI FFCLWVYI“gene 155.835/gene=“sAg“CDS 155.835/gene=“sAg“/function=“envelope protein“/codon_start=1/product=“small surface antigen“/protein_id=“CCC82736.1“/db_xref=“GI:341823529“/translation=“MENITSGFLGPLLVLQAGFFLLTRILTIPQSLDSWWTSLNF LGGSPVCLGQNSQSPTSNHS
13、PTSCPPICPGYRWMCLRRFIIFLFILLLCLIFLLVLLD YQGMLPVCPLIPGSTTTSTGPCRTCTTPAQGNSMFPSCCCTKPTEGNCTCIPIPLSWA FAKYLWEWASVRFSWLSLLVPFVQWFVGLSPTVWLSAIWMMWYWGPSLYSIVSPFIPL LPIFFCLWVYI“gene 1374.1838/gene=“X“CDS 1374.1838/gene=“X“/function=“putative transactivating protein“/codon_start=1/product=“HBx“/protein_id=“C
14、CC86611.1“/db_xref=“GI:342186698“/translation=“MAARLYCQLDPSRDVLCLRPVGAESRGRPLSGPLGTLSSPS PSAVPADHGAHLSLRGLPVCAFSSAGPCALRFTSARCMETTVNAHQILPKVLHKRTLG LPAMSTTDLEAYFKDCVFKDWEELGEEIRLKVFVLGGCRHKLVCAPAPCNFFTSA“gene 1814.2458/gene=“preC/C“CDS 1814.2458/gene=“preC/C“/function=“soluble secreted antigen“/codo
15、n_start=1/product=“HBeAg precursor“/protein_id=“CCC82737.1“/db_xref=“GI:341823530“/translation=“MQLFHLCLIISCTCPTVQASKLCLGWLWGMDIDPYKEFGAT VELLSFLPSDFFPSVRDLLDTASALYREALESPEHCSPHHTALRQAILCWGELMTLAT WVGNNLQDPASRDLVVNYVNTNMGLKIRQLLWFHISCLTFGRETVLEYLVSFGVWIRT PPAYRPPNAPILSTLPETTVVRRRDRGRSPRRRTPSPRRRRSQSPRRRRSQSRESQC“gene 1901.2458/gene=“C“CDS 1901.2458/gene=“C“/function=“capsid protein“/codon_start=1/product=“cAg“/protein_id=“CCC82738.1“/db_xref=“GI:341823531“/translation=“MDIDPYKEFGATVELLSFLPSDFFPSVRDLLDTASALYR
