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1、Commonly used strainsThis page describes some of the most commonly used yeast lab strains. Much of the information is taken from F. Sherman (2002) Getting started with yeast, Methods Enzymol. 350, 3-41. Other useful papers for strain background information include: Mortimer and Johnston (1986) Genet
2、ics 113:35-43 - thoroughly describes the genealogy of strain S288C van Dijken et al. (2000) Enzyme Microb Technol 26:706-714 - compares various characteristics of commonly used lab strains Winzeler et al. (2003) Genetics 163:79-89 - uses SFP (single-feature polymorphisms) analysis to study genetic i
3、dentity between common lab strainsS288CGenotype: MAT SUC2 gal2 mal mel flo1 flo8-1 hap1 ho bio1 bio6Notes: Strain used in the systematic sequencing project, the sequence stored in SGD. S288C does not form pseudohyphae. In addition, since it has a mutated copy of HAP1, it is not a good strain for mit
4、ochondrial studies. It has an allelic variant of MIP1 which increases petite frequency. S288C strains are gal2- and they do not use galactose anaerobically.The S288C genome was recently resequenced at the Sanger Institute.References: Mortimer and Johnston (1986) Genetics 113:35-43.Sources: ATCC:2045
5、08BY4743Genotype: MATa/ his31/his31 leu20/leu20 LYS2/lys20 met150/MET15 ura30/ura30Notes: Strain used in the systematic deletion project, generated from a cross between BY4741 and BY4742, which are derived from S288C. As S288c, these strains have an allelic variant of MIP1 which increases petite fre
6、quency. See Brachmann et al. reference for details.References: Brachmann et al. (1998) Yeast 14:115-32.Sources: Biosystems:YSC1050FY4Genotype: MATaNotes: Derived from S288C.References: Winston et al. (1995) Yeast 11:53-55.Brachmann et al. (1998) Yeast 14:115-32.FY1679Genotype: MATa/ ura3-52/ura3-52
7、trp163/TRP1 leu21/LEU2 his3200/HIS3 GAL2/GALNotes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: Winston et al. (1995) Yeast 11:53-55.Sources: EUROSCARF:10000DAB972Genotype: MAT X2180-1B trp10 rho 0Notes: Isogenic to S288C; used in the systemati
8、c sequencing project, the sequence stored in SGD. AB972 is an ethidium bromide-induced rho- derivative of the strain X2180-1B-trp1.References: Olson MV et al. (1986) Proc. Natl. Acad. Sci. USA 83:7826-7830.Sources: ATCC:204511A364AGenotype: MATa ade1 ade2 ura1 his7 lys2 tyr1 gal1 SUC mal cup BIONote
9、s: Used in the systematic sequencing project, the sequence stored in SGD.References: Hartwell (1967) J. Bacteriol. 93:1662-1670.Sources: ATCC:208526XJ24-24aGenotype: MATa ho HMa HM ade6 arg4-17 trp1-1 tyr7-1 MAL2Notes: Derived from, but not isogenic to, S288CReferences: Strathern et al. (1979) Cell
10、18:309-319DC5Genotype: MATa leu2-3,112 his3-11,15 can1-11Notes: Isogenic to S288C; used in the systematic sequencing project, the sequence stored in SGD.References: Broach et al. (1979) Gene 8:121-133X2180-1AGenotype: MATa SUC2 mal mel gal2 CUP1Notes:S288c spontaneously diploidized to give rise to X
11、2180. The haploid segregants X2180-1a and X2180-1b were obtained from sporulated X2180References: Mortimer and JohnstonSources: ATCC:204504YNN216Genotype: MATa/ ura3-52/ura3-52 lys2-801amber/lys2-801amber ade2-101ochre/ade2-101ochreNotes: Congenic to S288C (see Sikorski and Hieter). Used to derive Y
12、SS and CY strains (see Sobel and Wolin).References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.YPH499Genotype: MATa ura3-52 lys2-801_amber ade2-101_ochre trp1-63 his3-200 leu2-1Notes: Contains nonrevertible (deletion) auxotrophic mutations
13、that can be used for selection of vectors. Note thattrp1-63, unlike trp1-1, does not delete adjacent GAL3 UAS sequence and retains homology to TRP1 selectable marker.gal2-, does not use galactose anaerobically. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carl
14、son, Columbia University), which is congenic with S288C.References: Sikorski RS and Hieter P (1989) Genetics 122:19-27.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources: ATCC:204679YPH500Genotype: MAT ura3-52 lys2-801_amber ade2-101_
15、ochre trp1-63 his3-200 leu2-1Notes:MAT strain isogenic to YPH499 except at mating type locus. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which is congenic with S288C.References: Sikorski RS and Hieter P (1989) Genetics 122:19-2
16、7.Sobel and Wolin (1999) Mol. Biol. Cell 10:3849-3862.Johnston M and Davis RW (1984) Mol Cell Biol 4(8):1440-8.Sources: ATCC:204680YPH501Genotype: MATa/MAT ura3-52/ura3-52 lys2-801_amber/lys2-801_amber ade2-101_ochre/ade2-101_ochre trp1-63/trp1-63 his3-200/his3-200 leu2-1/leu2-1Notes: a/ diploid isogenic to YPH499 and YPH500. Derived from the diploid strain YNN216 (Johnston and Davis 1984; original source: M. Carlson, Columbia University), which
