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1、浓缩胶(5% Acrylamide)各种组分名称1ml2ml3ml4ml5ml6ml8ml10ml双蒸水0.681.42.12.73.44.15.56.830%Acrylamide0.170.380.50.670.831.01.31.71.0M Tris-HCl(PH 6.8)0.130.250.380.50.630.751.01.2510%过硫酸铉(AP)0.010.020.030.040.050.060.080.110% SDS0.010.020.030.040.050.060.080.1TEMED0.0010.0020.0030.004 0.0050.0060.0080.01分离胶(3)
2、 10%Gel各种组分名称5ml10ml15ml20ml25ml30ml40ml50ml双蒸水1.84.05.97.99.911.915.919.830%Acrylamide1.73.37.96.78.310.013.316.71.5M Tris-HCl(PH 8.8)1.32.53.85.06.37.510.012.510%过硫酸铉(AP)0.050.10.150.20.250.30.40.510% SDS0.050.10.150.20.250.30.40.5TEMED0.002 0.0040.0060.0080.010.0120.0160.02(4) 12%Gel各种组分名称5ml10ml
3、15ml20ml25ml30ml40ml50ml双蒸水1.63.84.96,68.29.913.216.530%Acrylamide2.04.06.08.010.012.016.020.01.5M Tris-HCl(PH 8.8)1.32.5.3.85.06.37.510.012.510%过硫酸铉(AP)0.050.10.150.20.250.30.40.510% SDS0.050.10.150.20.250.30.40.5TEMED0.002 0.0040.0060.0080.010.0120.0160.02(5) 15%Gel各种组分名称5 ml10ml15ml20ml25ml30ml40ml50ml双蒸水1.12.33,44.65.76.99.211.530%Acrylamide2.55.07.510.012.515.020.025.01.5M Tris-HCl(PH 8.8)1.32.53.85.06.37.510.012.510%过硫酸铉(AP)0.050.10.150.20.250.30.40.510% SDS0.050.10.150.20.250.30.40.5TEMED0.002 0.004 0.006 0.0080.01 0.012 0.016 0.02注意：浓缩胶用5ml, 15%的分离胶用10ml如有侵权请联系告知删除，感谢你们的配合!
