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1、基因BLAST及发育树构建操作步骤整理-周志勤1、输入“ http:/blast.nebi.nlm.nih.gov ”网址。得到：图2、单击 nucleotide blastBLAST Assembled GenomesFind Genomic BLAST pagEnlr igrga-riism name 申 iid-c口 mpletiGO i口Hejitiehio Raibibil口忑色brafkhMouse口 Chioipaawed 和iRato Guinea 旳口aZlraibkfoiMCcw口 Fruit ilyHi匸芒13业1 Hongv b盹B.fassL口Dog口 Chicken
2、MicmbeE单击土 nucleotide blastChiaase a BLAST program toi lic Bali uta bEOtE油 bls就Baste BLAST誓刖dh 吕 nwcleeride daftgtas? using n nuctegti-de qwery AenWms- M 自飢m mag自 bh聲t dittntigucus mblasESearch pol皂in daEabae u荀ng a protein query4修onThm占.blastp. psi-blast, phi-blast, delta-blast图13、得到新页面：图 24、 复制粘贴测
3、序公司提供的DNA序列到blastn窗口5、 勾选“结果显示在新窗口”，单击“blast”Edii and ResubfiutSearch Slral&qie Formattinci ontians DGWTiEDad鈕帕和 Hcisv := r&adNucleotkle Sequence (1466 letters)KIDAMZW5Mnjai4 rtxDirefln 12-13 12:39 de、Query ID Oecrlptton Mailecule type Query LengthId 126615None nucleic 军 fid1466得到此新页面Database NameDe
4、scriptlojaPrcgrajnnrNudeotide coflection (nt)BLASTN 2-2.50+ fr 亡nationOther reports:自5总耳由 勺uiEimrvt冷xcnoE” FFCJcirt弓 1 |卩冷1：曰c匚p bfp 口于J BLAST*Hera RtowntteutuSihmd SUaleiM Hrtpi MCOV OLASTr Hrln wMtM 刑 n f.iilp wenwtnStandMd Nucleotide BLASTBUlSTN programi MafcH mdcEeobiM(laLabausir|j a HuclotKle O
5、Mnr.mixe.Enter Qu$fyEier acceMjon numbef(i). gi), w FASIA 9equenL&(9.,5Y詞由rn俨UFromToOr. itpMffil 皆怜Job Tiilo整載DN/Vk基理空可 咕貼列此.处Errter a deBOW&ve Wt. tor ur SLjST seafdi yAlign two or me矗 MqwanttlChoose Search setDatJibnseHumrk gnOirYC + trrlSChpt胡如号或常/)如甘C * EfSnSC n pl 。Others nr 憾瞪 Program SBtedJon
6、Opflmlzv Iem*BLASI芒 /J j?_h| 叩pr-i r. ：i-f| HflWy umdirimtgabOsi)O Morv dfHnilir guiencri (*stmiflM0V9 nwiblotlSww*Mt simrt*fibiBtin)ChM9B i blast ngcHBim #单击BLASTfth du Cabins Hlii: Ivolid* d4 lection i|nr.rnl) ulMiy M4*gablMI (CplPini* Ibf highly 事irnilbi Mqutiricci reimlUi M 4 Aew wwMtew勾选6、得到新窗口
7、：图 3BLASTHcmtwRcccnl RejuhsSaved StrategksHelp WCEH.! BLAST/Masln suilei FormaUing 阳石ult& 8MZWHG 汕討E)Graphic SununnaryDistributKin erf 200 日曲 Hite on the Query Sequence .0Ibu苦噸lo 删and 駅cira$. click toJilignm廿rrt$Color kev for align merit scr&s|=200 |111I1250500750100012507、下拉页面，如：图 4&选择 Accession下的基
8、因编码。入选第一个“ HQ71佃83.1单击Accession下血的漏W扇 enpii&nMrcECr$To1?l sccir口卿叩 ccwrMineXBaoaus sutUliB sffalnBaiV5l 16S nbosarnai Rf-w nene. DaitlaissauencB26912&91m00100%HQ71130 3.1Batdiua 赴恤偌I阳iu芒诵亡泄rig strain ESR9 165Rl1. aefie.代帀刮世俪SfiSK9SK0.0Kug阳黑B 1uius a.T?iiQiqu*dacien5 slmin *ii口iSritsc-sciijlRHii. 口审n
9、#.nmrh;il sequEnca2礙2迹0093%FJ43&iO6.1nBackus so.165 ribosomal RNA nene, eartiai2692220.0曲Eiiu客 祖1虑morti%lrain QMADjs i6 ribosomal f4A aer?*. ca/lial aauene#2战2期0.09%時诈122 1吕jiHuw wuDhliw strain atCC iJJlT s佃山2 口qiktmq2&90诩阳200CPIKMI74B.1EJBacJus nMoliouladenB SCR9 coTOlete- oerKine269017030 0GEflOfl
10、aMLl；AJignrnwnlsSequences prQTgn羽 significant aljgranents: 思自I就I EJJ伽口冒SdtLed D9、得到新窗口如：图 510、单击 FASTASfDi雪pla* StlinqiS： G&nBankBacillus subtilis strain Bawsl 16S ribosomal RNA gene, partial sequenceLOCUSDEFINITIONHQ7119S31479 bp DMABCT 08-FEB-20IIBacillus subtilis st ram Bawsl 16S ribosonal KNA ge
11、nej. parti alsequenceFinds SLutksequences or patterns in the sequence aiACCESSION HQ711983VERSTON HO71 1 GT： 321 ?7?Fi7l=i11、得到如：图612、复制菌株种名跟16SrDNA序列，并粘贴到“ .txt”文件 中R&soLirce-s How ToNucleotideNucleotideAj/ancedRjsp 怙曽 SeitTiruiT： wFASTA导制鬲吨名字和基因編玛 记事本吗 partialBacillus subtilis strain BawsT 16S rib
12、osomal RNA gene，partial sequenceGrBankj-IQ71l9aa.lGEriEiHrik GNDhiz 呂gi|3212725T61gb|HQ711983. 1| Bacillus subtilis strain Bavsl IBS ribosanalsequenceTCGGCGTGCTATACATCCAACTCGACCGCXACATCCGACCTTGCTCCCTGATGTTACCGGCGGACGCGI GAG TAACACG TGGG TAACC TGCCTG TAAGACTGGGATAACTCCGWAAACCGGGGC T AATjLCCGG JLTGG L
13、 TGT T TGAACCGCATGG T TCAGACATAAAAGG TGGCTTCGCCTACCACTTACAGArGGACCCGCSGCGCAT TAGCTAG T TGG TGAGG TAACGGCTCACCAAMCGACGATGCGTAGCCUACCTGAGAGGG TGATCGGCCAC MTGGGACTGAGACMGGCOSAGACTcXTAg；嬴CCACC 鵰 T 岡茶 AATCTTHGCAATGGACQAAAG TCTGiCGGASCAlCGCCGCG TG AG TGATG 3GTTTTOGGATOGTAMfiCTCTGTTGTTlGGG 血血* AGTGCCG T TC
14、AAATAGGGCGGCACCTTGACGGTACCTAACCAGAAA&CCACGGCTAAC ?ACG TGCCAGCA GCCGCGG TAATACG TAGG TGGCAAGCGTTGTCCGGAATTATTGWCGTAAAGGGCTCGCAGGCGG TTTC7 TAAC TC TGATG TG 山lAC(XCCO：GCTC：AACt：gXAGG；Ti：ATTC AAMTGGGG AAC： TTGAC TCCACAA GAGGAG AG TGG AAT TCCACG TG TAGCGG TGAAATGCG TMATGTGCA&GAACACCAG TGGCGAAGGCG ACTCTC TGG TC TG TAKTGAOGCTGAGGAGCGAAGTGGWAKGAACAGGATTAGATACCC TGG TAG TCCACGCCGTAAACGATGAGTGCTAAGTGTTJJGGGGGTTTCCGCCCCTTAGTGCTGCK&CTAACGCA
