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1、Muscle Tissue 肌组织Ying Ding Email:The contentnVCD: The function of skeletal musclenObservation:nSkeletal muscle 骨骼肌 HE, NO.21 special stain shown LCT in yellow color 荧光桃红 NO.22(2) Cardiac muscle 心肌 HE, NO.19 special stain shown intercalated disc 铁苏木素染色看闰盘 NO.20nSmooth muscle 平滑肌 HE, NO.23nHomework: D
2、raw the longitudinal and cross section of skeletal muscle fiber in high power objective lens 40, and describe its construction features.n作业：在高倍镜下描绘骨骼肌纤维的纵、横切面，并 描述其结构特征。 肌组织的分类、分布：分类分布光镜骨骼肌心 肌平滑肌躯干、四肢心 脏内脏、血管壁横纹肌横纹肌无横纹收缩随意肌 快 不持久不随意肌 有节律 持久不随意肌 慢 持久(1)Skeletal muscle 骨骼肌 HE NO.21nNaked eye: The sect
3、ion of tongue is pink color.n肉眼：舌头切面，染成粉红色nLowest power objective lens 4: The most tissue is stained in pink.n低倍镜4*：表面凹凸不平，深蓝色的为舌 黏膜上皮，深面为骨骼肌，大部分染成粉 红色。Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of skeletal muscle fibers. 低倍镜10*：纵、斜、横切
4、面Skeletal muscle fibers (lower magnification)Cross sectionLongitudinal sectionBlood vessel (oblique section)Lower power objective lens 10: This is longitudinal section of skeletal muscle fibers. Compare the staining of muscle tissue with LCT. What are their differences? 低倍镜10*：骨骼肌的纵切面。 试比较其与疏松结缔组织的不
5、同。Skeletal muscle fibers (longitudinal section, lower magnification)(skeletal muscle fibers)loose connective tissueHigh power objective lens 40: Analyses the structure in different sections of individual skeletal muscle fiber. Multi nuclei are peripheral location just underneath of the sarcolemma, w
6、hich can be seen in various sections. (the nucleus of skeletal muscle fiber)(skeletal muscle fiber)a skeletal muscle fiber(cross section)myofibrils(cross section)myofibrils(longitudinal section)loose connective tissue高倍镜40*：分析不同切 面的骨骼肌特征。多核，位于肌纤维的周边， 紧靠肌膜内面，不同切面均 可见。High power objective lens 40: In
7、the longitudinal of skeletal muscle fiber the myofibrils are very thin parallel with the length of the cell. And identify its cross alternative light and dark band (cross stria) with the diaphragm closed down. 40*镜纵切面：与 长轴平行的细丝为肌原纤维。缩小光圈区分明暗相间的横纹-明暗带 。(the nucleus of skeletal muscle fiber)(skeletal
8、muscle fiber)(loose connective tissue)cross striaHigh power objective lens 40: In the cross section of skeletal muscle fiber the myofibrils are stippled (dot) appearance. The delicate LCT surrounding each skeletal muscle fiber can be seen, which is termed endomysium. 40*横切面：肌原纤维呈细点状结 构，周围包绕的细致疏松结缔组织
9、为肌内膜。a skeletal muscle fiber (cross section)myofibrils (cross section)myofibrils (longitudinal section)绘图格式 图名， 染色方法，放大倍数图题 ？标出骨骼肌 某些典型结 构的名称用简单的话描述其结构特征Special stain: In order to easily define muscle tissue with CT, using this special stain to recognize them. In this staining the muscle tissue is i
10、n red, CT is in yellow. 特殊染色（荧光桃红）： 肌组织红色，结缔组织黄色。Special stain:Skeletal muscle fibers (red)Loose connective tissue (yellow)(2) Cardiac muscle心肌 HE NO.19; special stain, NO.20nNaked eye: The section of the wall of heart is pink color.n肉眼：心脏切面染成粉红色。nLowest power objective lens 4: Move the slide you wi
11、ll find various sections of cardiac muscle fibers. n低倍镜4*：找到不同切面的心肌纤维。通过比较观察心肌的形态结构（光镜 ）n肌纤维整体形态n肌浆（胞浆）n胞核n有无特殊结构n肌纤维间的结缔组织Lower power objective lens 10: To identify various sections of the cardiac muscle fibers. Among the cardiac muscle fibers there is less amount of LCT and rich capillaries. Look
12、for the perfect cross and longitudinal section of cardiac muscle fibers. Then turn to Lens 40 . 10*不同切面。肌纤维之间有少量疏松结缔组织 和毛细血管。找到典型的横、纵切面转到高倍镜下观察。blood vessleblood vessleLoose connective tissuecross sectionCardiac muscle (low magnification)longitudinal sectionHigh power objective lens 40: Compare to t
13、he skeletal muscle fibers. In the longitudinal of cardiac muscle fibers, which are branching and anastomosing with others, the cross-striated banding pattern is visible but not so clear than that of skeletal muscle fibers. The nucleus is located in the center, and surrounding by light cytoplasm due
14、to without myofibrils. Try to find intercalated disc, which is darkly stained straight or step-like line crossing the fibers at interval.Cardiac muscle fibers (longitudinal section-high magnification)intercalated discThe nucleus of cardiac muscle fibercross-striated bandblood vessle40*纵切面：心肌 纤维呈短柱状，
15、 有分叉，互相吻 合。明暗相间的 横纹不如骨骼肌 明显。核呈卵圆 形，位于细胞中 央，两端无肌原 纤维，被浅染的 胞浆包绕。在心 肌纤维连接处染 色较深的结构为 闰盘，呈直线或 者梯形。High power objective lens 40: In the cross section of cardiac muscle fibers, they are different size and appearance, the large and round appearance with or without centrally nucleus, the large and pear-like
16、appearance with or without centrally nucleus, and the small and round shaped only with stippled appearance myofibrils.loose connective tissueblood vessela cardiac muscle fiber (cross section)40*横切面 ：肌纤维呈 大小不一的 块状。细胞 核位于中央 ，核周围淡 染。肌纤维 切面有的比 较大，圆形 或梨形，伴 或不伴细胞 核，有的比 较小，呈圆 形，仅为斑 点状的肌原 纤维。Special stain: In section NO.20, the intercal
