《Acute phase plasma and brain tissue content of insulin-like growth factor-1 on experimental cerebral hemorrhage（急性期血浆和脑组织内容的胰岛素样生长因子- 1在实验性脑出血）》由会员分享，可在线阅读，更多相关《Acute phase plasma and brain tissue content of insulin-like growth factor-1 on experimental cerebral hemorrhage（急性期血浆和脑组织内容的胰岛素样生长因子- 1在实验性脑出血）（13页珍藏版）》请在金锄头文库上搜索。
1、1Acute phase plasma and brain tissue content of insulin-like growth factor-1 on experimental cerebral hemorrhageAuthor: Lvyun Li Fu Xuefeng Road Kui Liu Jun Xu Wenwu Keywords rats Keywords: rat; cerebral hemorrhage; insulin-like growth factor-1; brain tissue; plasma Abstract: Objective To investigat
2、e the insulin-like growth factor -1 (IGF-1 levels in the acute phase of experimental cerebral hemorrhage in rats plasma and brain tissue changes clarify the role in the process of cerebral hemorrhage 80 Methods SD rats were randomly divided into control group, a small amount of cerebral hemorrhage g
3、roup, in the amount of cerebral hemorrhage group and a large number of brain hemorrhage group, n = 20, n = 1,8,24,48 and 72 h after the hemorrhage were divided into five groups, each group of four. using Nath rat cerebral hemorrhage model, the content of plasma and brain tissue IGF-1 was measured by
4、 radioimmunoassay. compared with the control group, large, medium, small amount of cerebral 2hemorrhage in cerebral hemorrhage, the 1h plasma IGF- 1 began to reduce, but no significant difference, 8, 24 and 48h decreased significantly (P 0.01, dropped the most obvious to 8h, 72h has been restored to
5、 1h level, compared with the control group, cerebral hemorrhage, 8, 24 and 48h perihematoma organization IGF-1 were significantly increased (P 0.01,8 h peak, then gradually decreased to close to the control group, 72h., in a small amount of cerebral hemorrhage group in 8,24 and 48 h as compared Ther
6、e are very significant difference (P 0.01. conclusions of IGF-1 may be involved in the pathophysiological process of cerebral hemorrhage, a new factor. Keywords: rat; cerebral hemorrhage; insulin-like growth factor-1; perihemotoma; plasma Abstract: AIM To observe insulin-like growth factor-1 (IGF-1c
7、oncentration change in plasma perihemotoma of rats with acute cerebral hemorrhage in order to explore the effect of IGF-1on acute cerebral hemorrhage.METHODS Eighty SD rats weighted250 300g were randomly assigned to four groups: control group, and little, medium, large quantity hemorrhage 3groups wi
8、th each group divided into1, 8,24,48 and72h post hemorrhage subgroups.The model of cerebral hemorrhage was copied according to Naths and IGF-1contents in rat plasma and perihemotoma were mea-sured with radioimmunoassay.RESULTS Compared to con-trol group, plasma IGF-1content began to decrease after a
9、-cute cerebral hemorrhage, with no significant difference at1and72h, significant difference (P 0.01at8, 24and48h post cerebral hemorrhage, especially at8h ; and with no sig-nificant difference at the same time-points during three exper-iment groups.Compared to control group, IGF-1contents in perihem
10、otoma appeared statistically significantly higher dur-ing48h post acute hemorrhage (P 0.01, at the peak at8h , but72h post hemorrhage not significantly higher.Among three experiment groups, IGF-1contents in large quantity hemorrhage group appeared significantly higher than that in little and medium
11、hemorrhage ones from8to48h post hem-orrhage (P 0.01.CONCLUSION IGF-1might be in-volved in the pathogenesis of acute cerebral hemorrhage. 0 Introduction 4Insulin-like growth factor -1 (insulin-like growth factor-1, IGF-1 to promote growth and development, and to promote material metabolism, lower blo
12、od sugar, and promotes cell proliferation role. Mediated growth hormone because it has also been called growth interleukin its cell biological effects through its receptor With the development of molecular biology and its role in the nervous system, has been widely attention in 1997, Schwabs 1 for t
13、he first time report more than 20 cases of acute ischemic stroke patients plasma IGF-1 level was significantly lower than the control group, and that it may have an important role in the pathophysiological process of acute ischemic brain damage and treatment, but IGF-1 in acute cerebral hemorrhage c
14、ontent changes in plasma and hematoma surrounding brain tissue has not been reported rat model of intracerebral hemorrhage, dynamic observation of acute cerebral hemorrhage in the brain tissue IGF-1 levels, and to explore IGF-1 in cerebral hemorrhage significance in the pathophysiological process. 1
15、 Materials and methods 80 1.1 Materials healthy male SD rats (Experimental 5Animal Center of the Fourth Military Medical University, body weight 250 300g, were randomly divided into a control group, a small amount of cerebral hemorrhage group, in the amount of cerebral hemorrhage group and a large n
16、umber of brain hemorrhage group, each 20, each group was divided into after hemorrhage, 1,8,24,48 and 72h, 5 group, each group of four. 1.2 Methods Nath et al 2 ip anesthesia of the rats with pentobarbital sodium (30mg kg-1, the prone position is fixed in the stereotaxic apparatus to find the anterior fontanelle 0.2mm, 3mm at the the midline right side next to the open drill diameter 1.5mm round hole, the depth of the dural surface, along the hole micro
