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1、HAPSITE ER/Smart Plus TrainingHAPSITE ER/Smart Plus 培 训,Advanced ER IQ/Plus IQ 高级的 ER IQ/Plus IQ,Course Outline 课程大纲,Overview 综述 Instrument Setup 仪器设置 Tune 调谐 Troubleshooting 故障查找 Advanced Data Review 高级数据处理 Creating AMDIS Libraries 创建 AMDIS 库 Recognizing Co-Elution 识别共淘析 Chromatogram Overlay 色谱图迭加
2、Accessing Quantitation Reports 查访定量报告 Basic Method Writing 基本方法编写 Method Editor Overview 方法编写器概述 SIM Methods SIM 方法 Method Sequencing 方法序列 Blank Subtraction 空样扣除 Communications 通讯,Instrument Setup 仪器设置,Insert Gas Canisters 插入气瓶 Turn on the Analytical Module or take the Analytical Module out of Exten
3、ded Standby 将分析模件电源 on 或将分析模件离开延伸的待机状态 System will heat, AutoTune and run a Concentrator cleanout 系统将加热, 自动调谐和运行浓缩器清理 Turn on the Laptop 将计算机电源 on Double click the ER IQ/Plus IQ icon to open the software 双击 ER IQ/Plus IQ 图标, 打开软件,Tune Overview 调谐概述,Tune optimizes the performance of the MS 调谐使 MS 的性能
4、最佳化 An improperly tuned MS can result in: 未正确调谐的 MS 可导致: Low sensitivity 灵敏度低 Undetected compounds 未能检测到化合物 Mass misalignment 质量数未对准 Incorrect identifications 不正确的 ID 鉴别 Incorrect Mass Ratios 不正确的质量比 Low Net Fit or SI numbers 低纯拟合或 SI 数,Tune Overview 调谐概述,Ten masses are used when tuning the MS 调谐 MS
5、 使用 10 个质量数 Masses are between 50-282 to encompass the full 41-300 range of mass fragments 质量数在 50-282 之间包含整个 41-300 质量分裂物范围 The Internal Standard gas contains the chemicals that provide these masses used for tuning 内部标准气体包含提供这些用于调谐质量数的化合物,AutoTune 自动调谐,The HAPSITE will automatically AutoTune: HAPSI
6、TE 将自动调谐: Upon Startup 当启动时 Upon exiting Extended Standby 当退出延伸的待机状态时 After 12 hours of operation运行 12 个小时后,AutoTune 自动调谐,AutoTune can be initialized by: 自动调谐可由下列动作起始 : Clicking Tune AutoTune on the laptop 在计算机上单击 Tune AutoTune Touching RUN AUTOTUNE on the front panel 触摸面板上的 RUN AUTOTUNE 按钮 Rebootin
7、g the HAPSITE 重新启动 HAPSITE,Troubleshooting a Failed AutoTune 不成功自动调谐的故障查找,Internal Standard Canister 内部标准气瓶 Verify that the pressure is adequate 核实压强是否适当 Verify the expiration date 核实是否已到使用期限 Insert new IS canister if necessary 必要时插入新的 IS 气瓶 Carrier Gas (Smart Plus Only) 载气 (仅 Smart Plus) Verify pre
8、ssure that the pressure is adequate 核实压强是否适当 Reservoir pressure ( 400 kPa) 贮存器压强 ( 400 仟帕) ER does not have a reservoir ER 无贮存器 Insert new Carrier Gas canister if necessary 必要时插入新的载气瓶 Repeat AutoTune 重新执行自动调谐,Tune Requires Advanced Mode 调谐要求高级模式,The Access Level must be set to Advanced to manually t
9、une the instrument 必须将 Access Level 设定于高级, 手动调谐仪器 Set Access Level is used to change the ER IQ/Plus IQ user mode Set Access Level 用于更改 ER IQ / Plus IQ 的用户模式 Normal Mode: Allows access to default methods and data analysis 常规模式: 允许进入默认方法和数据分析 Advanced Mode: Allows access to method editing, manual tuni
10、ng, file transfer and file deletion 高级模式:允许进入方法编写, 手动调谐, 文件传送和文件删除,ER IQ/Plus IQ Set Access LevelER IQ/Plus IQ 设定查访层次,Select Set Access Level 选 Set Access Level Select Advanced from the drop down menu 从下拉菜单上选 Advanced Neither access level is password protected under the default settings 默认设定查访层次无口令保
11、护 Advanced mode can be password protected if desired 必要时可用口令保护高级模式 Click OK 单击 OK,Set Access Level 设定查访层次,Manual Tune 手动调谐,Double click the Tune icon 双击调谐图标 Select 选 default.tun Click OK 单击 OK Note: The NEG is being consumed while Manual Tune is open 注: 在打开手动调谐的同时, 消耗 NEG,Base Peak Gain (BPG) 基峰增益 (
12、BPG),BPG is related to the intensity of mass 117 BPG 与 M 117 的强度有关 BPG influences sensitivity BPG 影响灵敏度 ER ideal setting is 0.5 ER 理想的设定值为 0.5 Range is 0.4 to 0.6 范围为 0.4 至 0.6 Smart Plus ideal setting is 1.0 Smart Plus理想的设定值为 0.1 Range is 0.8 to 2.0 范围为 0.8 至 2.0,Adjusting Base Peak Gain调整基峰增益,The
13、BPG is adjusted using EM (Electron Multiplier) voltage 用 EM (电子倍增器) 电压调整 BPG Adjust using increments of 25 V 用增量 25 伏调整 The normal operating range is 1000 to 2000 volts 常规工作范围为 1000 至 2000 伏,Adjusting Base Peak Gain (BPG)调整基峰增益 (BPG),Adjusted Base Peak Gain for an ER 为 ER 调整基峰增益,Real Time Mass Calib
14、ration Table实时质量校准表,Red boxes may be present in the Real Time Mass Calibration Area 在实时质量校准区可出现红色框 This indicates that resolution and/or intensity of the mass is out of range 这表明质量数的分辨率和/或强度已超出范围,Status Column 状态栏,Status Column will read OK, OK Low, OK High, Low or High 状态栏将出现 OK, OK 低, OK 高, 低 或 高等
15、读值 High and Low are not acceptable 高和低是不可接受的,Mass Alignment 质量对准,Perform a Mass Alignment by pressing F5 按 F5 执行质量对准 Updates the Status column readings 更新状态栏读值 Perform a Mass Alignment prior to and after making adjustments to the tune parameters 调整调谐参数前和后执行质量对准,Target Resolution 靶分辨率,Resolution refers to the width of the mass 分辨率与质量数的宽度有关 The mass width should be 1 AMU 质量数宽度应为 1 AMU The range for the Target Resolution is 0.85 and 1.10 靶分辨率的范围在 0.85 至 1.10 之间,Adjusting Target Resolution 调整靶分辨率,Adjust in increments of 0.05 调整的增量为 0.05 The number in the Actual Percentage column is affected b
