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1、第三军医大学硕士学位论文兔骨髓基质细胞移植在心肌内存活分化及对心功能的影响姓名：蒋世忠申请学位级别：硕士专业：内科学（心血管病）指导教师：黄岚20030501第三军医大学硕士学位论文英文缩写AMIDMEMFBSDABDAPIPBSNBTMVDISECMHEDEPCTrisTEMEDIBMXPILVEFLVEDVLVESVHRFSMSCsIESDLVEDD英文缩写一览表英文全称acutemyocardialinfarctiondulbeccomodifiedeglesmediumfetalbronesemmdiaminobenzidine4，6diamidino一2一phenylindoleph
2、osphatebufferedsalinenitrobluetetrazoliummicrovasculardensityinfarctionsizeextracellularmatrixhematoxylinandeosindiethylprocarbenatetrihydroxymethylaminomethaneN，N，N，N-tetramethylethylenediamine3-Isobutyl一1一methylxanthineIodizedleftventricularejectfractionleftventficularenddiastolicvolumeleftventric
3、ularendsystolicvolumeheartratefractionalshorteningmarrowstromalcellsleftventricularendsystolicdiameterleftventricularenddiastolicdiameter中文全称急性心肌梗死DMEM培养基胎牛血清3，3一二氨基苯联胺4，6-氨基2苯茚二酮磷酸盐缓冲生理盐水四唑氮蓝毛细血管密度梗死面积细胞外基质苏木精和伊红焦碳酸二乙酯三羟甲基氨基甲烷N，N，N，N四甲基乙二胺3一异丁基一1一甲基黄嘌呤碘化丙啶左室射血分数左室舒张末期体积左室收缩末期体积心率短轴缩短率骨髓基质细胞左室收缩末期内径
4、左室舒张末期内径兰三兰堕盔兰堡主堂垡笙塞一RabbitbonemarrowstromalcellstransplantedinmyocardiumsurvivalanditsaffectionontheheartfunctionAbstractBackgroundandobjectiveMyocardialinfarctionisoneofthediseasethatcanharmoneSheathandcauselethaleventsCoronaryflowsuppliedbycollateralvesselsandcompensationofnecrosismyocardiummaypr
5、otecctthemyocardiumfromischemia，reduceinfarctionsize，savethemyocardiumOntheriskofdeathTheexperimentalstudiesaim：1TotestthehypothesisthatbonemalTowstromalcells(MSCs)，whenimplantedintomyocardium，canundergomiliumdepcnendentdifferentiationandsurvivalinthemyocardialmuscle2TouattheeffectoftheimplantedMSCs
6、onthefunctionalrestorationofdamagedheart，promoteangiogenesisofischimiccardiacmuscle，improvedthesupplyofbloodflowforischimiccardiacmusclesThefirstpartTheseparationofbonemarl-owstromalcells，amplificationinvitro，anditsbiologicalcharacterSternalbonemarrowstromalcellswasdrewfromdonerrabbitlegbones，andMSC
7、swereculture-expanded，amplification，tracedMSCslabledwith46一diamidino一2一phenylindole(DAPI)WeobservedbiologicalcharacterofMSCs，andidentifiedmulti-potentialdifferentiationofMSCsintolipoblastinvitrounderappropfiteenvironment。ThesecondpartMSCsmicroenvironmentdependentdifferentiationincardiacmuscleMSCsiso
8、latedfromdonerrabbitlegboneswerecultureexpanded，labeledwithDAPI，andtheninjectedintothemyocardiumofthesamerabbitTheheartswereharvestedfrom1dayto6weeksafterimplantatedTheimplantedsiteswereexaminedtoindentifythemigrationandsurvivalofimplantedcells，observethechangesofthelabledimplantedmarrowstmmaleellsT
9、hethirdpartMSCseffectiononreconstitutionandrepeatedpouratonofinfractedcardiacmuscle，andrecoveryofcardiacfunctionEstablishthemodelofrabbitacutemyocardialinfarctionMSCsdrawfromrabbitswerecultureincellcuturemedium，labeledwithDAPIEmployingarabbitischemicheartmodeling，MSCswereinjecteddirectlyintotheische
10、micareaandthenumberofvesselswasexaminedimmunohistochemicallyusingtheantiCD31monoclonalantibodyCardiacfunctionwasmeasuredbyechocardiography2第三军医大学硕士学位论文_-_一一beforethecellimplantationand1week，4weeksafterMSCstransplantedThepathologicalchangesofischemiccardiacmusclewereobservedResultsThefirstpartMostofc
11、ulturedMSCswereadherenttothecultureplatewithin24hours，theadherentcellswereseenascoloniesonlyafewcellson57days，bytheendof1214daysthecoloniesofadherentcellshadexpandedin8090percentsizeofthecultureplateAdhenenttwice-passedMSCshadsimilarmorphologiccharacteristics，mostbeingfibroblasticinappearance88perce
12、ntculturedMSCsinstaticstagecellcycle，about96percentwerealiveUnderelectronmicroscope，TheculturedMSCswereseenasprimaryunmaturephenotype7daysdyingwithoilred，mostdifferentiatedceilswerepositiveThesecondpartViablecellslabeledwith4，6-diamidino-2-phenylindolecanbeindentifiedintheimplantedsitesatalltimepoin
13、tsafterimplantatedTheimplantedmarrowstromalcellsshowthegrowthpotentialinamyocardialmicroenvironmemtAfter6weeks，donercellscontainedsarcomeresandwereconnectedbyjunctionscomposedofdesmosomesandfasviaadherensThethirdpartLabledMSCssurvivedinischemJcmyocardium；labledMSCswerefoundwithinthewallofsmallvessel
14、sinareaadjacenttOmyocardialinfarction，thecardiacfunctionoftheMSCsgroupwashigherthanthatinmediuminjected(EFofMSCspromptedO46+003VS0504-003P005)：FSwashigherthanthatinmediuminjected(2385169VS27024-127PO05)：reducedtheinfarctsize(4431OVS1372O92)：lightmicroscpicanalysisofthevesselcountpositivelystainedbya
15、ntiCD31intheischemicareashowedthatangiogenesishadbeeninducedtOasignificantlygreaterdegreeinthegroupimplantedwithMSCsthanthatinthegroupinjectedwithcellculturemedium4weeksafterMSCsimplantedtransplanted(16300+2585VS96004-1661P005)：MSCsinducedangiogenesisintheischemicmyocardiumareaPathologicalmorphologi
16、cinMSCstransplantedheartsbeUerthanthatinthecontrolheartsConclusionThefirstpartMSCswereeasyisolatedfromdonerlegbonesbyaspirationMSCsmaybeculturedeexpansedinvitro，andexpressunmaturephenotypeMSCsdifferentatiouintointerstitialtissueunderappropriatecondition，demonstratedmultipotentialdifferentationalcapacityThesecondpartMarrowstromalcellsasdo
