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1、 微卫星微卫星DNA和和AFLP标记在水稻标记在水稻分子标记连锁图上的分布分子标记连锁图上的分布 1熊立仲熊立仲1王石平王石平1刘克德刘克德1戴先凯戴先凯 2M.A.SaghaiMaroof3胡锦国胡锦国1张启发张启发 1(华中农业大学作物遗传改良国家重点实验室武汉华中农业大学作物遗传改良国家重点实验室武汉430070) 2(Department of Crop and Soil Environmental Science, Virginia Polytechnic Institute and State University, VA24061, USA) 3(Department of ve
2、getable Crops, University of California, Davis, CA91616,USA) 评论员：李宇歌评论员：李宇歌 摘要摘要 以一个栽培稻以一个栽培稻(Oryza sativa L.ssp.indica) 和野生稻和野生稻(O.rufipogon Griff)杂交的杂交的2作图群作图群体以及由该群体构建的体以及由该群体构建的RFLP标记连锁图标记连锁图,分析分析了微卫星了微卫星DNA和和AFLP标记的多态性、遗传行为标记的多态性、遗传行为及其在染色体上的分布。共定位了及其在染色体上的分布。共定位了28个微卫星个微卫星标记和标记和172个个AFLP标记。标记。
3、28个微卫星个微卫星标记中有标记中有6个为华中农业大学作物遗传改良个为华中农业大学作物遗传改良国家重点实验室根据数据库中序列而设计国家重点实验室根据数据库中序列而设计,其余其余22个来自美国个来自美国Cornell大学已发表的结果。大学已发表的结果。172个个AFLP标记出自标记出自25对引物扩增得到的对引物扩增得到的228个多态个多态性带的片段。这些标记分布于水稻的性带的片段。这些标记分布于水稻的12条染色条染色体。将此体。将此200个个PCR标记与华中农业大学作物遗标记与华中农业大学作物遗传改良国家重点实验室构建的传改良国家重点实验室构建的RFLP连锁图整合连锁图整合,得到一张含得到一张含
4、612个分子标记位点的遗传连锁图。个分子标记位点的遗传连锁图。 关键词关键词 SSR,AFLP,RFLP,基因图谱基因图谱 Abstract SSR(simple sequence repeat) and AFLP (amplified fragment length polymorphism) are PCR based molecular markers developed in recent years. In this study, the authors analyzed the polymorphisms, inheritance and distribution of SSR a
5、nd AFLP markers using an F2 population from across between cultivar “Aijiao Nante” (Oryza sativa L. ssp. Indica) and an accession of the common wild rice (O. rufipogon Griff). A total of 200 new markers were obtained including 28 SSR and 172 AFLP markers. Six of the 28 SSR markers were developed by
6、National Key Laboratory of Crop Genetic Improvement(NKLCGI) using DNA sequences from GenBank and the other 22 were from data published previously. The 172 AFLP markers were from a total 228 polymorphic bands amplified using 25 selected primer combination. Mapping of the 200 new markers using NKLCGIS
7、 previously developed RFLP map based on the same F2 population resolved these markers to all 12 rice chromosomes. Integration of the SSR and AFLP markers into the RFLP map resulted in a high density molecular linkage map containing 612 polymorphic loci.标题标题: 微卫星微卫星DNADNA和和AFLPAFLP标记在水稻分子标记在水稻分子标记标记连
8、锁图上连锁图上的分布的分布 DISTRIBUTION OF SIMPLE SEQUENCE REPEAT AND AFLP MARKERS IN MOLECULAR LINKAGE MAP OF RICE 改正：改正：方案一：方案一：SSR和和AFLP标记再水稻分子标记连锁图上标记再水稻分子标记连锁图上的分布的分布方案二：方案二：Distribution of Microsatallite DNA and AFLP Markers in Molecular Marker Linkage Map of Rice不一一对应不一一对应 中文摘要第一句话是中文摘要第一句话是 “以一个栽培稻以一个栽培稻
9、(Oryza sativa L.ssp.indica) 和和野生稻野生稻(O.rufipogon Griff)杂交的杂交的2作图作图群体以及由该群体构建的群体以及由该群体构建的RFLP标记连锁图标记连锁图,分析了微卫星分析了微卫星DNA和和AFLP标记的多态性、标记的多态性、遗传行为及其在染色体上的分布遗传行为及其在染色体上的分布”。 英文摘要第一句是英文摘要第一句是 SSR(simple sequence repeat) and AFLP (amplified fragment length polymorphism) are PCR based molecular markers deve
10、loped in recent years。介绍介绍SSR和和AFLP这两个分子标记技术。这两个分子标记技术。第二句第二句以一个栽培稻以一个栽培稻(Oryza sativa L.ssp.indica) 和野生稻和野生稻(O.rufipogon Griff)杂交的杂交的F2 作图群体以及由该作图群体以及由该群体构建的群体构建的RFLP标记连锁图标记连锁图,分析了分析了微卫星微卫星DNA和和AFLP标记的多态性、遗传行为及其在染标记的多态性、遗传行为及其在染色体上的分布。色体上的分布。In this study, the authors analyzed the polymorphisms, in
11、heritance and distribution of SSR(替换为替换为Microsatallite DNA) and AFLP markers using an (加上加上RFLP marker linkage map based on the)F2 population from across between cultivar “Aijiao Nante” (Oryza sativa L. ssp. Indica) and an accession of the common wild rice (O. rufipogon Griff).用词不当用词不当 28个微卫星标记中有个微卫
12、星标记中有6个为个为华中农业大学华中农业大学作物遗传作物遗传改良国家重点实验室根据数据库中序列而改良国家重点实验室根据数据库中序列而设计设计,其余其余22个个来自来自美国美国Cornell大学大学已发表的结果。已发表的结果。 Six of the 28 SSR markers were developed(替换为替换为designed) by National Key Laboratory of Crop Genetic Improvement(NKLCGI) (加上加上 of Huazhong Agriculture University) using DNA sequences from
13、GenBank and the other 22 were taking from data previously published.(加加上上 by Cornell University.) 设计引物不是构建引物，应将设计引物不是构建引物，应将develop 改为改为design 在作物遗传国家重点实验室后加上所属华中农大，其余在作物遗传国家重点实验室后加上所属华中农大，其余22个来自康奈尔大学也应该注明，因为中文摘要中有。个来自康奈尔大学也应该注明，因为中文摘要中有。这些标记分布于水稻的这些标记分布于水稻的12条染色体。将此条染色体。将此200个个PCR标记与华中农业大学作物遗传改良国家
14、重点实验室构标记与华中农业大学作物遗传改良国家重点实验室构建的建的RFLP连锁图整合连锁图整合,得到一张含得到一张含612个分子标记位个分子标记位点的遗传连锁图。点的遗传连锁图。 Mapping of the 200 new markers using NKLCGIS previously developed RFLP map based on the same F2 population resolved these markers to all 12 rice chromosomes. Integration of the SSR and AFLP markers into the RFL
15、P map（加加上上that was developed by the NKLCGI of Huazhong Agriculture University )resulted in a high density molecular linkage map containing 612 polymorphic loci.改改为为： The 200 new markers distribute to all 12 rice chromosomes.Distribution of Microsatallite DNA and AFLP Markers in Molecular Marker Link
16、age Map of Rice Abstract SSR(simple sequence repeat) and AFLP (amplified fragment length polymorphism) are PCR based molecular markers developed in recent years. In this study, the authors analyzed the polymorphisms, inheritance and distribution of Microsatallite DNA and AFLP markers using an RFLP m
17、ap based on the F2 population from across between cultivar “Aijiao Nante” (Oryza sativa L. ssp. Indica) and an accession of the common wild rice (O. rufipogon Griff).A total of 200 new markers were obtained including 28 SSR and 172 AFLP markers. Six of the 28 SSR markers were designed by National Ke
18、y Laboratory of Crop Genetic Improvement(NKLCGI) of Huazhong Agriculture University using DNA sequences from GenBank and the other 22 were taking from data previously published by Cornell University. The 172 AFLP markers were from a total 228 polymorphic bands amplified using 25 selected primer combination. The 200 new markers distribute to all 12 rice chromosomes. Integration of the SSR and AFLP markers into the RFLP map that was developed by the NKLCGI of Huazhong Agriculture University resulted in a high density molecular linkage map containing 612 polymorphic loci.
