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1、Cleavage Sites Table for Fusion Proteins：PROTEASECOMPANYProtease CaptureThrombinLeu-Val-Pro-ArgGly-SerAmersham-Biosciences, Novagen,SIGMA, RocheBenzamidine-AgaroseFactor XaIle-Glu/Asp-Gly-ArgAmersham-Biosciences, New England Biolabs,RocheBenzamidine-AgaroseEnterokinaseAsp-Asp-Asp-Asp-LysNew England
2、Biolabs,Novagen, RocheTrypsin Inhibitor-AgaroseTEV proteaseGlu-Asn-Leu-Tyr-Phe-GlnGlyInvitrogen Life TechnologiesNi-NTA (6His recomb. TEV)PreScissionLeu-Glu-Val-Leu-Phe-GlnGly-ProAmersham-BiosciencesGSTrap for GST fusion enzymeTAGZymeHis-tag removal by Exoproteolytic DigestionQiagenNi-NTA (6His reco
3、mb. enzyme)Intein Sitedithiothreitol cleavageNew England BiolabsDTT elimination by dialysisHRV 3C ProteaseLeu-Glu-Val-Leu-Phe-GlnGly-ProNovagen Ni-NTA (6His recomb. enzyme)SUMO Proteaserecognize the tertiary structure of the ubiquitin-like (UBL) protein, SUMOInvitrogen Life Technologies, LifeSensorsNi-NTA (6His recomb. enzyme)
