Western Blot凝胶配制分离胶配方表：不同凝胶体积所对应各种组分的量5mL10mL15mL20mL25mL30mL40mL50mL6%H2O2.65.37.910.613.215.921.226.530% Acrylamide1.02.03.04.05.06.08.010.01.5M Tris-Hcl（pH8.8）1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.048%H2O2.34.66.99.311.513.918.523.230% Acrylamide1.32.74.05.36.78.010.713.31.5M Tris-Hcl（pH8.8）1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.0410%H2O1.94.05.97.99.911.915.919.830% Acrylamide1.73.35.06.78.310.013.316.71.5M Tris-Hcl（pH8.8）1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.0412%H2O1.63.34.96.68.29.913.216.530% Acrylamide2.04.06.08.010.012.016.020.01.5M Tris-Hcl（pH8.8）1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.0415%H2O1.12.33.44.65.76.99.211.530% Acrylamide2.55.07.510.012.515.020.025.01.5M Tris-Hcl（pH8.8）1.32.53.85.06.37.510.012.510%SDS0.050.10.150.20.250.30.40.510%过硫酸铵0.050.10.150.20.250.30.40.5TEMED0.0040.0080.0120.0160.020.0240.0320.04浓缩胶配方表（5%）各种凝胶体积所对应各种组分的量1ml2ml3ml4ml5ml6ml8ml10mlH2O0.681.42.12.73.44.15.56.830%Acrylamide0.170.330.50.670.831.01.31.71.0M Tris-Hcl（pH6.8）0.130.250.380.50.630.751.01.2510%SDS0.010.020.030.040.050.060.080.110%过硫酸铵0.010.020.030.040.050060.080.1TEMED0.0010.0020.0030.0040.0050.0060.0080.01常用配方表：分离胶（35mL，四块1.5mm的胶，每块胶7.5-8mL）各组分体积（mL）组分名称8% 分离胶12%分离胶10%分离胶H2O16.211.513.830%Acrylamide9.31411.71.5M Tris-Hcl（pH8.8）8.88.88.810%SDS0.380.380.3510%过硫酸铵0.350.380.35TEMED0.0280.0280.0285%浓缩胶（14mL，四块1.5mm的胶，每块胶约3mL）组分名称体积（mL）H2O9.530%Acrylamide2.371.0M Tris-Hcl（pH6.8）1.7510%SDS0.1410%过硫酸铵0.14TEMED0.01410电泳（转膜）缓冲液 ： Tris 30.3g 甘氨酸 144g SDS 10 g用ddH2O定容到1000mL，常温储存1电泳缓冲液 ： 10电泳缓冲液 100mL ddH2O 990mL 10%SDS 10mL 充分混匀 1转膜缓冲液： 10转膜缓冲液 100mL ddH2O 700mL 无水甲醇 200mL 充分混匀，放置4℃保存。
10TBS缓冲液Tris-Hcl 24.2gNaCl 80g蒸馏水至 1000mL1TBS-T 10TBS缓冲液 100mL ddH2O 900mL Tween-20 0.5mL。