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1、医学微生物学实验,第三次 外界因素对细菌的影响与细菌的变异,热力灭菌(高压、干烤),紫外线杀菌作用(操作) 碘酒酒精对皮肤的消毒作用,空气细菌培养(每室一套) 药敏试验(操作,抗生素对细菌作用及耐药变异) 观察噬菌体的特异溶菌现象,变形杆菌的迁徙现象及对照 接种脓汁标本接种血平板(每组一个,下次实验用),Methods for control of microorganisms,Physical methods Heat Hot-air sterilizer Autoclaving Radiation Filtration Ultrasound Dryness Low temperature
2、Chemical agents,Hot-air sterilizer 55-60C for 30-60min 160-170C for 2 hours-spores Glass petri dishes and pipettes Autoclaving 15 pounds(1.05kg/cm2), 121C for 15-20min Ultraviolet, UV 250-260nm Quite lethal but does not penetrate glass, dirt films, water, and other substances very effectively. To sterilize the air and any exposed surfaces Can burn the skin and damage eyes,碘酒酒精对皮肤的消毒作用,空气细菌培养(每室一套),