《micrifluidic chips for point 病原菌检测新技术》由会员分享,可在线阅读,更多相关《micrifluidic chips for point 病原菌检测新技术(4页珍藏版)》请在金锄头文库上搜索。
1、Fabrication of Microchips for LAMP:,integrate the LAMP on a microfluidic chip microLAMP (LAMP)This kind of inlet/outlet was necessary for the convenient and accurate addition of the DNA sample and at the same time making the capillary force available to transport the LAMP reaction mixture into micro
2、channel.The final dimension of the microchannel is 1 mm 0.8 mm 0.6 mm with a volume of 5 L.A reaction mixture for LAMP (prepared manually according to the system above) of 4.6 L was drawn slowly into the microchannel by capillary force.,Sensitivity and Specificity of the LAMP:,We ascribe the strong turbid signal in the microchamber to its larger depth-to-width ratio (DWR of the microchamber in our LAMP and a typical PCR tube was 1.33 and 1.00, respectively).,LAMP for Quantitative Analysis:,A single channel optical detection module,