《骨髓细胞学检查英文课件(第一部分)》由会员分享，可在线阅读，更多相关《骨髓细胞学检查英文课件(第一部分)（53页珍藏版）》请在金锄头文库上搜索。
1、Testing of bone marrow Testing of bone marrow cytology (1)cytology (1)1 1. The generation, . The generation, development and normal development and normal morphology characteristics of morphology characteristics of blood cellblood cell2 21. Generation of blood cells1. Generation of blood cells All b
2、lood cells come from common hemopoietic stem cell(HBSC). HBSC has two characteristics: 1)the high capability of self-renewal to keep the constancy of the quantity of the stem cell pools; 2)the capability of multi-differentiation. HBSC can differentiate into progenitor cells of every series.3 3Stem c
3、ellErythropoietin(EPO)Red progenitor cellGranulocyte- macrophage colony stimulating factor(GM- CSF)Granulocyte- macrophage progenitor cellGiant nuclear progenitor cellMeg-CSF and thrombopoietin (TPO)4 4Mononuclear cellsMove into various tissues Histiocytes, or phagocytesT lymphoid progenitor cellsB
4、lymphoid progenitor cells B-lymphocytesImmune cellsMitogen and antigenT-lymphocytesLymphatic stem cellsPlasma cells5 52. General principles of 2. General principles of morphological evolution during morphological evolution during the development of blood cellsthe development of blood cells6 6 With t
5、he development and maturity of blood cells: (1)Cell volume: from big to small (2)Cytoplasm: 1)volume increased 2)Color turns from deep blue to light blue, even carmoisine 3)Granules in cytoplasm: no granulesazurophil granulesspecific granules(neutral, acidophilic and basophilic strippling)7 7(3)nucl
6、eus: 1)Size: from big to small From regular to irregular, even with sublobes 2)Chromatin: from fine to rough, tight and dense, color changes from light to deep 3)Nucleole: clearblurreddisappeare d 4)Nuclear membrane: unclearclear8 8(4)ratio of cell nucleus and cell-substance From big to small, i.e.
7、from big nucleus with little cytoplasm to small nucleus with abundant cytoplasm(except megakaryocyte)9 91010Evolution of blood cells, 1000x1111Normal morphologic Normal morphologic characteristics of blood cellcharacteristics of blood cell1. Erythrocyte series1212shapeDiameterNucleusNucleus diameter
8、/c ell diameterChromatinNucleoleCytoplasmGranulesNormoblastRound or elliptical15-22mround4/5Fine sand- like of fine grain-like1-5, lasureous, with unclear limitLittle, opacitas, deep blueNoBasophilic erythroblastRound or elliptical11-20mroundOver 2/3Small condensatenoMore, opacitas and deep blueNoPo
9、lychomati c normoblastRound or elliptical8-18mround1/2Bolus or rope- like, ranking like wheels, with clear olistherozonenoMore, shows polychromaticity in different degree with inhomogeneous color Ortho- chromatic normoblastRound7-12mroundBelow 1/2noMuch, steady salmon pink or extremely light gray pu
10、rple Reticulocyteround8-9mnonoContains residual rRNA that can be stained granulated blue erythrocyteBiconcave disc7.2mnonoPink, weakly stained in the center1313 (1)normoblastSometimes on the edge of cell there are hemisphere or tumor-like ecphymas. Sometimes there is olistherozone surrounding the nu
11、cleus with light color.1414 (2)basophilic erythroblastThere are still tumor-like ecphymas and olistherozone surrounding the nucleus.1515 (3) Polychomatic normoblast1616 (4) Ortho-chromatic normoblast1717 (5) ReticulocyteThe reticulocyte is an incompletely mature red blood cell after the orthochromat
12、ic normoblast loses its nucleus. 1818 (6) erythrocyte19192. Granulocyte series2. Granulocyte series(1)myeloblast Shape: round or elliptical Diameter: 11-18m nucleus constitutes over 2/3 of cell volume Nucleus shape: round or elliptical Chromatin: fine-sand-like, light prunosus, smooth like gossamer
13、Nucleole: 2-5, clear, light blue or colorless Cytoplasm: little, clear sky blue around the nucleus Granules: no or a few2020(2)promyelocyte Shape: round or elliptical Diameter: 12-22m Nucleus: big, round or oval Chromatin: thick granular or net -like, distributing unevenly Nucleole: clear or disappe
14、ared Cytoplasm: comparatively much, light blue or blue Sometimes there is olistherozone near the nucleus Granules: non-specific azurophilic granules, distributed unevenly, with different size, shape and quantity2121Comparison of 3 kinds of Comparison of 3 kinds of myelocytemyelocyteShapeDiameter/mCh
15、romatinNucleoleCytoplasmgranulesNeutrophilic myelocyteround10-18Net-like or tablet-likeNoAbundant, salmon pinkSteady, tiny and light purple specific neutral granules Eosinophilic myelocyte15-20Net-like or tablet-likeNoFull of thick, steady, ranking tightly and refractive orange acid specific granule
16、s Basophilic myelocyte10-15vagueA few thick, purple black and scattered specific basophilic granules with different sizes2222Neutrophilic myelocyteThe nucleus constitutes 1/2-2/3 of the cell volume2323Eosinophilic myelocyte2424Because of the basophilic granules in the cytoplasm, the nucleus is unclear and the structure of the chromatin is vag
