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1、SICENCE 新闻发布 作者：杨俊峰 稿号：NRR_260_14脊髓损伤后神经再生障碍：脊髓损伤后神经再生障碍：Nogo-A 随时间变化的动态表达随时间变化的动态表达目前认为脊髓损伤后神经再生困难与中枢神经系统内髓磷脂关联抑制蛋白表达增加相关，其中Nogo-A 被认为是最主要的神经生长抑制因子。来自中国南京中医药大学的杨俊峰所在课题组，希望应用免疫组织化学染色、Western Blot、实时荧光定量 PCR 的方法，进一步检测损伤脊髓组织Nogo-A 的动态表达规律。发现脊髓损伤后 24 h，Nogo-A mRNA、蛋白表达和免疫阳性反应下降，3 d 后下降至最低，7 d 后迅速上升达到高峰
2、，至 14 d 再逐渐下降。结果显示，大鼠脊髓损伤后，Nogo-A 在早期一过性下降后可持续保持高水平表达。作者认为，这一结论可进一步揭示 Nogo-A 是造成脊髓损伤后中枢神经系统神经再生困难的重要原因之一。文章发表在中国神经再生研究（英文版） 杂志 2015 年 2 月第 2 期。Article:Article: “ Nogo-ANogo-A expressionexpression dynamicallydynamically variesvaries afterafter spinalspinal cordcord injuryinjury,“ by Jianwei Wang1, Ju
3、nfeng Yang1, Yong Ma2, Zhen Hua2, Yang Guo1, Xiaolin Gu2, Yafeng Zhang1 (1 Wuxi Hospital of Traditional Chinese Medicine, Institute of Orthopaedics and Traumatology of Nanjing University of Chinese Medicine, Wuxi, Jiangsu Province, China; 2 Nanjing University of Chinese Medicine, Nanjing, Jiangsu Pr
4、ovince, China)Wang JW, Yang JF, Ma Y, Hua Z, Guo Y, Gu XL, Zhang YF (2015) Nogo-A expression dynamically varies after spinal cord injury. Neural Regen Res 10(2):225-229.欲获更多资讯：Neural Regen Res Time-dependent Nogo-A expression is an obstacle for neuroregeneration after SCIIt has been considered that
5、neuroregeneration obstacle after spinal cord injury (SCI) correlates with the increase in myelin-associated growth inhibitory protein expression. Nogo-A is considered a major nerve growth inhibiting factor. Jun-feng Yang, Nanjing University of Chinese Medicine, China used real-time quantitative PCR,
6、 immunohistochemistry and western blot analysis to further investigate the dynamic expression law of Nogo-A in the injured spinal cord. Twenty-four hours after spinal cord injury, Nogo-A protein and mRNA expression was low in the injured group compared with control and sham-operated groups. The leve
7、ls then continued to drop further and were at their lowest at 3 days, rapidly rose to a peak after 7 days, and then gradually declined again after 14 days. These changes were observed at both the mRNA and protein level. The transient decrease observed early after injury followed by high levels for a
8、 few days indicates Nogo-A expression is time dependent. This may contribute to the lack of regeneration in the SICENCE 新闻发布 作者：杨俊峰 稿号：NRR_260_14central nervous system after spinal cord injury. The relevant article was published in the Neural Regeneration Research (Vol. 10, No. 2, 2015).Article: “ N
9、ogo-A expression dynamically varies after spinal cord injury,“ by Jian-wei Wang1, Jun-feng Yang1, Yong Ma2, Zhen Hua2, Yang Guo1, Xiao-lin Gu2, Ya-feng Zhang1 (1 Wuxi Hospital of Traditional Chinese Medicine, Institute of Orthopedics and Traumatology of Nanjing University of Chinese Medicine, Wuxi, Jiangsu Province, China; 2 Nanjing University of Chinese Medicine, Nanjing, Jiangsu Province, China)Wang JW, Yang JF, Ma Y, Hua Z, Guo Y, Gu XL, Zhang YF (2015) Nogo-A expression dynamically varies after spinal cord injury. Neural Regen Res 10(2):225-229.
