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1、國立屏東教育大學 96 學年度研究所碩士班入學考試生物學 試題（應用化學暨生命科學系碩士班）請注意：答案須寫在答案卷，否則不予計分。一、解釋名詞：(10%)1. Active Transport2. Stem Cell二、何謂細胞週期、有絲分裂及減數分裂？說明在減數分裂各期中染色體變化的情形。(25%)三、請說明光合作用中，光反應與暗反應的反應機制。(25%)四、Please compare the differences between the eukaryotic and the prokaryotic cell in （1）chromosomal DNA （2）cytosol compo
2、sition (10%)五、If you have T2 bacterial phage, isotope P32, S35, host of T2, and everything needed to label isotope on target, please describe how do you prove viral genetic materials are nucleic acid (Hershey and Chase)? (10%)六、Here we have 2 antigens（A and B, 7 epitopes/each）and each shares 3 same
3、epitopes, if you want to diagnose their difference, you will use polyclonal or monoclonal antibody? Why? And how will you “make” your chosen antibody if no one sells it? (15%)七、In eukaryotic cell，pre-mRNA will become mature and transport to the cytosol. Except splicing，what will be added to the mRNA
4、？(5%) Does there have any relationship between these “added components” and RNA PolII？(5%) What is alternative splicing？(5%)八、What are Southern blot, Northern blot, Western blot and immuno-gold localization？What can they tell you from each experimental data？(20%)九、Left figure is pUC19 plasmid vector，please describe why these units（genes or DNA fragment）are put into pUC19。If you want to clone a DNA fragment into pUC19，how do you know your insert (the DNA fragment) is really there? (20%)（另附彩色頁於後）
