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1、Chapter6酶EnzymeJ【盅w1enzymesascatalysts;2.factorsaffectingenzymeactivity;:3.enzymekineticsandinhibition;T人a随eecn真E政浩怀E丶1877年,Kuhne首次提出Bnzyme一词。1897年,Buchner兄弟用不含细胞的酵母提取液,实现EE刑人e朋1982年,Cech首次发现RNA也具有酶的催化后性)提出2与EEE标E阮0刑小接酶活性DNA片段,称为脱氧校酶(deoxyribozyme)。酶(enzyme):catalyststhatchangetherateofareactionwith
2、outbeingchangedthemselyves.VirtulallyallenzymesareDLofteins,althoughsomeh标沥JI核酶(ribozyme):具有催化作用的核酸chemicalequilibriaEsJinchutoturconr(Substrate)productSection1MolecularstructureandactivesiteJ1.MolecularstructureEnzymeTsimpleoneconjugatedoneLu+nonprotein=holoenzyme叩岫喵晶nChUtg靓cOn蓼伽咖n)organiccompound买
3、inorganicImetalionsQOOIaBIeSeOwnthesamecoenzyme:1四D动NAD+hbutdifferentapD0EnZyIme.malicdehydrogenaseJEbub一白苹果酸胺氢酶Malicacid十NAD*Eb005英e国志英wpoenzyme:一一determinesthespecificityofthecatalyticsystemt:E坂transferchemicalgroups(electron,atom,chemicalgroups)betweenenzymiesE(酶的活性口心)Essentialgroup(必需基团):Active具墓暮珏墅妻tl蛊重禀黯支暴慧雍I蔓墨lsubstr髓tesbindandundergoachemicalreaction结合基团(Dindinggroup)催化基团(catalyticgroup)
