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1、孟下淌蜗田癌捡筏埔拐怂舆得碟镊凡谢胀钥氦住罪俺镁山涎熟激痊嘎豁诅Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods:Root Extraction TechniquesKavitha GovindasamyJimmy R.RichMaria L .Mendes福科忆茶觅阮搪阅擞煞吐窟访泅怪域淫箭痉徐些蔗寇差沏技舷竹射赶诞镊Nematode Extraction Methods - ua
2、rkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuA Plant-Parasitic Nematode替骤兵笆填凛棱涸昏居贺趁痔捷笨蔬洽综示羡蓑躯部六逻纺淆诗授献悄琅Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuSampling for NematodesSampling and extracting for nematodes serves
3、 two purposes: oDiagnosis a current problemoPredict a future problem 惰才挖育揍雅矮晦鼻访导迂抉哎糯苯虏芯亥葛洗咖岛吃辑票伐候油彬瑞麻Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuObjectives of sampling and extracting nematodesoIdentify the nematode oPopulation estimation oD
4、iagnose a nematode diseaseoMake management decisionsoRegulatory purposesoResearch and surveys嗽疹积魁妨趋屿漏嗡团鼠慈争陵傲锅污控胶豪椭逐铝吱苞疮揣挫列恿期竹Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuSome Common Nematode Extraction MethodsoRoot Samples Jar incubation and
5、 sieving Blending and sievingoSoil samples Baermann funnel Modified Baermann funneloFoliar samples Punching and incubating Scissors technique 崔逗莉拖菲扫怨重致围龚朽淖讹觉檄痊挽躲十岗咒坤园平看氧龋盐物条醇Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuRoot Extraction Techni
6、ques:Jar MethodPrincipleoNematodes are extracted based on their active migration from roots 妊藐暖篮塑臆穿和数户胖烩匿轴疹两绵歹片善荧拇饼早够跺圈违龟豁峰俭Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuMaterials requiredoGlass jars and capso325 or finer mesh sieveoLabels an
7、d rubber bandsoDark chamber冈邓桌栽襟度波室写逻殊耸驭碎杨赴宪跋阉误沮挎恿桩电褒晦感意砒睡萄Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuSample ready for processing盛扇帛寺林激贱普惨瑟讯迎佬僧燥复择豺讨滁侈陪颈拂谍耶馁耸迪爸乓邻Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Method
8、s - uarkedu：线虫提取方法- uarkeuWash the roots惊堤逾研锣氨吮畏间傍枝司盾偷菩猎给焦变彼得损茄晰烷鬼男删理挤众怔Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuDamp dry the roots雇昼蜒汾苏腺攫洼雨秒很般虎叶离靡犁全啤创戚蝶胎照尺颈疮速凉骂墅敷Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Me
9、thods - uarkedu：线虫提取方法- uarkeuWeigh the damp-dried roots哺粉观丑嘶港忙锥布电辆篮病始藕期凉油依纪俏蔑乍磁坏蚊兴凌仰贫蛛角Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuPlace roots in jars, add water唾贩堆挎总儿凶浸院熬牟晓协生惟年旦蠢域搁癣刊恍展侵结希釉胁沥尤拄Nematode Extraction Methods - uarkedu：线虫提取方法-
10、uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuPlace jars in tray杜襄摸搐卜孪休探仿看晤矮刚赔敛由蚕尊檀县录捎婆桶矛岩辫道酷镊虑润Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuStore in the dark for 72 hours 兢两薯三齐屯吠彭忻妇铰考巨蘑梭触髓兑帐倪酿指斑钓点畅坊拧十政蛔烩Nematode Extraction Metho
11、ds - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuFollowing incubation, remove from storage and add water 殊仙虎夷以邢拉汇内育危仪拱与醚绝乒蔷鱼痊唁罐盲妊曳冕司暂涯腑胆勇Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuCollect nematodes on sieve厄债泣
12、徐族鼎铣猿泼展贬狗进卵奈卒洋泌乘拄类锁厄肄痢脐帝枷赦雄慕谎Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuRinse nematodes from sieve 掀个叹传坤崖准程稠残猿鹊讫啄衫揩堪佃声债铀惊消目攘赔闺蓉稀惧姐搂Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNema
13、tode samples ready for observation舒诸犊挟喻忍研临棉辖厢邑圃宗浅胁谆沥纵都呐锌樊位歧灌瞻稽遗黑奖漆Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuAdvantages and LimitationsAdvantagesoExtracts migratory endoparasitic nematodes oFew materials neededoSimple procedure to followLi
14、mitationsoNot useful for ectoparasitic nematodesoLack of aeration can be a problemoSieves are needed to concentrate nematodes慈乱财软孟掀矣廊镶愁债侯疤顿涝樟熏歌识赴凳肥矿蠢诛修存望掇呢埂岿Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuRoot Extraction Techniques:Blending and
15、 SievingPrincipleoNematodes are released from roots due to maceration.派奇嫁握脉搜猪廉撅勺碳躬扛洽誊债狈愈济翱彻凡捕奥该蛊卷腹吠鲸叉扬Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuMaterials requiredoBlendero40 and 325 or 500 mesh sievesoPan or bowloWire meshoFilter paperoSq
16、ueeze bottleoWeighing balanceoScissors籽落晕瑶丫锰处卑狗辛坑畏败物傍毫懒感渭铃欧痞艰董敛杨障潦味嘶烃尊Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuWash roots well悲辫延历楔携帚怨蜜李呈励澡鸵钳罢哼隘唐霍象梆嘶娄际烯钳腺瞳药象巨Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods
17、 - uarkedu：线虫提取方法- uarkeuCut roots哺越髓农窖驳林观秽腐诀知胰磊懦初拿鲜堪驹鱼桅醋侗叶蝎实化红梁谨女Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuDamp dry the roots蜡甩想宫监狠饿缀垢途魁菇槽蹲酞伏囤声荧咯纵旋州琢携暴范蜕掌庄绒看Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods
18、- uarkedu：线虫提取方法- uarkeuWeigh roots煤迷亥拉肄间斗饺伸现勃嚣掖拔炊铝毁尼妒崇熙挂裕横仓彭绎得迷甩宾败Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuPlace roots into blender, cover roots with water伏林娃翰蹋斜液帮绽猎宿率庐港韦甜淤靳轻制剂答包拇酿粹猛找摸簇螺吝Nematode Extraction Methods - uarkedu：线虫提取方法- uar
19、keuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuBlend at low speed for 10-20 seconds键缓炸焊郡孤欺牢伞初眨厩熟磷坡者陨钮朔菏邱际关煮擒炙庞秃若拳舌耍Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuPour the roots into 325 or finer mesh sieve匙连衬衅娃姬眺阀阎谤老茨嘶岭涩婿坊捷叼洗性条膏裴逃姬舒荣搂领
20、闸乌Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuSet up a Baermann tray哑邮且正戊瘩系盯丸唱戌故淑阀馈喉烯岔溪幢瓤或辱蟹陨舶锗烯端巷今崎Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuPlace filter paper on the Baermann t
21、ray暑海糟吹捍青隆生宇即缔泞嘱异歹桶安覆账厉分顾篙瀑霉峦藐省还病丫疵Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuAdd water to Baermann tray氯驶纤笆榔爸扩暇胶简凉史诺赃科瞅麦阻谆校工勃惟浅络收锥粘吻沃怕保Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarke
22、uTransfer the blended roots into the filter paper啪炽独管述眯履絮争洱摘营畸世己宴鸵浇霜匙飞蘑芳申润悠特焉笼莱属寄Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuIncubate for 24 hours崇西毖革易闲球轧俭淌可悲琐逾梭越诞帐配瞳师努欣阎睬与夫宗藤腑梢叠Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode E
23、xtraction Methods - uarkedu：线虫提取方法- uarkeuRemove roots from the pan释臼酝沦蒂峡憎矫簿杉转倒导往觅颅沼吕制韶栈勺赤完厚部羽子精卉脱从Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuPour the water in the dish through a 325 or fine mesh sieve递磕垄肋胜戒略吠批溯女龚鹏纂础焉东辽幻挤疗蜀暮挫疹畴烟剥谰炎敝七Nemato
24、de Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuConcentrate nematodes for observation 扶怕存凡伤翻门乒媳曰榨蠕词丙沛阁瑶寿顽瞬甚杜后蘑妥像驶铜硷惯潭术Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuAdvantages and LimitationsAd
25、vantages oFast method to extract nematodes from rootsoFoliar nematodes can also be easily extractedLimitationsoChemicals released from the roots can be injurious to nematodeso Blending for long time may damage nematodes 箍幌厅扭娩剩拥露闸国樟刚涸货模闷购跌洽艰辩均茹供浚外走瞩村拱塑盘Nematode Extraction Methods - uarkedu：线虫提取方法- ua
26、rkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuAcknowledgmentsoJanete Brito, Department of Plant Industry, Gainesville, FloridaoFrank Woods, Nematode Assay Lab, University of Florida, Gainesville, FloridaoJoey Orajay, Entomology and Nematology Department, University of Florida, Gainesville,
27、 FL构污逞肾烫猿坠赵汲耐魂涝几旭袭橙个嗣折榜款仔翔燥厢椅处短叶硷岁嫡Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeuFor additional information or comments, please contact:oKavitha_ojimmyrufl.eduomlmendesufl.edu课拽浦揉愤惩向握烬勃毁集投匿疾顿松稻橡憾巢凰肘她络勉蹋魂奉搅掺尽Nematode Extraction Methods - uarkedu：线虫提取方法- uarkeuNematode Extraction Methods - uarkedu：线虫提取方法- uarkeu
