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1、绝对定量实验简介和绝对定量示例实验美国应用生物系统公司 7300/7500 实时定量 PCR 仪设计绝对定量实验Primer Extended on mRNA5Synthesis of 1st cDNA strand35 cDNAReversePrimerOligo d(T) or random hexamer5 cDNA3执行反转录运行绝对定量反应板分析绝对定量数据 Copyright 2004, Applied Biosystems. All rights reserved.For Research Use Only. Not for use in diagnostic procedure
2、s.Authorized Thermal CyclerThis instrument, Serial No_, is an Authorized Thermal Cycler. Its purchase price includes the up-front feecomponent of a license under United States Patent Nos. 4,683,195, 4,683,202 and 4,965,188, owned by RocheMolecular Systems, Inc., and under corresponding claims in pat
3、ents outside the United States, owned by F. Hoffmann-La Roche Ltd, covering the Polymerase Chain Reaction (PCR) process to practice the PCR process for internalresearch and development using this instrument. The running royalty component of that license may be purchasedfrom Applied Biosystems or obt
4、ained by purchasing Authorized Reagents. This instrument is also an AuthorizedThermal Cycler for use with applications licenses available from Applied Biosystems. Its use with AuthorizedReagents also provides a limited PCR license in accordance with the label rights accompanying such reagents.Purcha
5、se of this product does not itself convey to the purchaser a complete license or right to perform the PCRprocess. Further information on purchasing licenses to practice the PCR process may be obtained by contacting theDirector of Licensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster City
6、, California 94404.DISCLAIMER OF LICENSE: No rights for any application, including any in vitro diagnostic application, areconveyed expressly, by implication or by estoppel under any patent or patent applications claiming homogeneous orreal-time detection methods, including patents covering such met
7、hods used in conjunction with the PCR process orother amplification processes. The 5 nuclease detection assay and certain other homogeneous or real-timeamplification and detection methods are covered by United States Patent Nos. 5,210,015, 5,487,972, 5,804,375 and5,994,056, owned by Roche Molecular
8、Systems, Inc.; by corresponding patents and patent applications outside theUnited States, owned by F. Hoffmann-La Roche Ltd; and by United States Patent Nos. 5,538,848 and 6,030,787, andcorresponding patents and patent applications outside the United States, owned by Applera Corporation. Purchase of
9、this instrument conveys no license or right under the foregoing patents. Use of these and other patented processes inconjunction with the PCR process requires a license. For information on obtaining licenses, contact the Director ofLicensing at Applied Biosystems, 850 Lincoln Centre Drive, Foster Ci
10、ty, California 94404, or The LicensingDepartment, Roche Molecular Systems, Inc., 1145 Atlantic Avenue, Alameda, California, 94501, USA.TrademarksApplied Biosystems, MicroAmp, Primer Express, ROX, and VIC are registered trademarks of Applera Corporation orits subsidiaries in the US and/or certain oth
11、er countries.AB (Design), ABI PRISM, Applera, Assays-by-Design, Assays-on-Demand, Celera Genomics, FAM, iScience,iScience (Design), and MultiScribe are trademarks of Applera Corporation or its subsidiaries in the US and/or certainother countries.AmpErase, AmpliTaq Gold, and TaqMan are registered tra
12、demarks of Roche Molecular Systems, Inc.SYBR Green is a registered trademark of Molecular Probes, Inc.Microsoft and Windows are registered trademarks of Microsoft Corporation.All other trademarks are the sole property of their respective owners.Part Number 4347963 Rev. A3/2004ii7300/7500 实时定量 PCR 仪绝对定量实验入门指南3 30, 2004 10:02 , GSG_FrontCover.fmSAMPLE DOCUMENT t/ 1 1 t/77300/7500PCR t/ t/ t/72 t/-t t/- qG- -(RT-PCR)- 3 Primer Extended on mRNA5Synthesis of 1st cDNA strand35cDNAReversePrimerOligod(T)orrandomhexamer5cDNA3 RNA)RNA RNAcDNA(Primer Extended on mRNA5Synthesis of 1st
