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1、人(5-HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒人(5-HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒产品规格：96T/48T。供应商：上海乔羽生物有限公司上海乔羽有限公司，有elisa试剂盒，抗体，培养基人(5-HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒其主要特点如下专一性强：抗原与抗体的免疫反应是专一反应，而免疫酶技术以免疫反应为基础，所检测的对象是抗原 (或抗体)，使用的抗体除标记了酶以外，与普通抗体的免疫反应特性并无多大差别。灵敏度高：由于抗体联结上了酶，因此，借助于酶与底物的显色反应，显示抗原与抗体的结合，大
2、大提 高了检测的灵敏性，使检测水平接近放射免疫测定法。人(5-HIAA)ELISA试剂盒说明书,5羟基吲哚乙酸ELISA检测试剂盒从冷藏环境中取出应在室温平衡后方可使 用。1标准品复溶：试剂盒提供6管标准品，每管已标定浓度，并且冻干。实验前在每个标准品管中加入0.5mL 样本稀释液，盖好后静置10分钟以上，然后反复颠倒/搓动助其溶解，使其恢复为每个标准品管身标注的 浓度。2.20X洗涤缓冲液的稀释：蒸馏水按1： 20稀释，即1份20X洗涤缓冲液加19份蒸馏水。人(5-HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒试剂的准备：按试剂盒说明书的要求准备实验中需用的试剂。ELI
3、SA中用的蒸馏水或去离子水，包括用于洗涤的，应为 新鲜的和高质量的。自配的缓冲液应用pH计测量较正。从冰箱中取出的试验用试剂应待温度与室温平 衡后使用。试剂盒中本次试验不需用的部分应及时放回冰箱保存。ELISA试剂盒的优势：全面一一混合8种不同的常见抗原，对自身免疫性疾病进行全面筛查。快速检测时间短(2小时)，确诊时间更早准确ELISA检测方法，灵敏度高，特异性强，适用性好。质优一一美国技术开发，高品质及高可靠性的分析性能。价低一一国内自生产，符合国内实情，降低生产成本。人(5HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒ELISA technology principl
4、e:Based on immunological reaction, the spec ific reaction of antigen and antibody was combined with the high efficiency of enzyme to substrate.1 solid phase of antigen or antibody and enzyme marker of antigen or antibody.2 binding to the antigen or antibody on the surface of the solid supports to ma
5、intain its immunological activity.3 the enzyme labeled antigen or antibody not only retains its immunological activity, but also retains the activity of the enzyme.4 the antigen or antibody on the surface of the specimen and the solid carrier. Enzyme labeled antigen or antibody is then added to the
6、solid support.5 at this time, the amount of enzyme in the solid phase and the amount of the substance in the sample were in a certain proportion.6 after adding the substrate of the enzyme reaction, the substrate is catalyzed by the enzyme to be a colored product, and the amount of the product is dir
7、ectly related to the amount of the substance in the sample, so the qualitative or quantitative analysis can be carried out according to the color of the color. Determination of the method has a high sensitivity (pg-ng/ml level), and good reproducibility.人(5HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒产品特色优势：1.
8、 国内对照品行业领导者，长达14年对照品的专业研发及标准制定2. 高效稳定的专利纯化技术-高速逆流色谱技术的运用3. 特有的高速逆流色谱对照品研发中心及生产中心，保证对照品的质量可控性及批量生产的稳定性4.严格的质量控制，通过全面的检测(1HNMR 13CNMR MS HPLC) 人(5-HIAA)ELISA 试剂盒说明书，5 羟基吲哚乙酸 ELISA 检测试剂盒 Prospective application: ELISA method for quantitative determination of related substances in human serum, plasma, c
9、ell culture supernatant or other related biological fluids.1 Kit preservation: -20 C (a longer time to use); 2-8 C (frequent use).2 washing liquid preserved at low temperature there will be precipitation, heating the water to help dissolve when diluted.3 Chinese and English instructions may be incon
10、sistent, please refer to the specification in english.4 just open the enzyme linked plate hole may contain a little water samples, this is a normal phenomenon, will not have any effect on the results of the experiment.7 could not detect the NaN3 containing samples, because NaN3 inhibited the activit
11、y of horseradish peroxidase (HRP).人(5-HIAA)ELISA试剂盒说明书，5羟基吲哚乙酸ELISA检测试剂盒试剂盒性能1. 灵敏度：最小的检测浓度小于1号标准品。稀释度的线性。样品线性回归与预期浓度相关系数R值为0.990。2. 特异性：不与人其它细胞因子反应。3. 重复性：板内、板间变异系数均小于10%。结果判断与分析1、仪器值：于波长450nm的酶标仪上读取各孔的OD值2、 以吸光度OD值为纵坐标(Y)，相应的HLA-B27标准品浓度为横坐标(X)，做得相应的曲线，样品的 HLA-B27含量可根据其OD值由标准曲线换算出相应的浓度。3、检测值范围:0-8.0IU/ml4、敏感度：0.01 IU/mlQl 、1友情提醒：公司经营的产品均为科研实验用，不可用于临床应用。
