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1、非标记纳米金光谱法检测雌激素余玲 1,2，张彦峥3，王亚丹3，张银堂1，徐茂田1,3*1 商丘师范学院化学化工学院，河南 商丘， 4760002 中南大学化学化工学院，湖南 长沙，4100123 郑州大学化学与分子工程学院，河南 郑州，450001*Email: 本文利用可见光度法研究了纳米金粒子与雌酮的相互作用。首先利用经典的柠檬酸钠还原氯金酸法制备 得到了均一稳定的纳米金粒子，平均粒径为35 nm。在pH=5.72的BR缓冲体系中，纳米金与雌酮发生相互作 用，吸收光谱明显红移。吸光度与雌酮浓度的对数值在0.0110 xlO-6 molL-i范围内呈良好的线性关系，检 测限为4.7x10-9
2、molL-1。本研究建立了一种快速、方便检测雌激素的新方法，为奶粉、食品中雌激素的检测 提供了一条新的途径。Fig. 1 Structure of estroneFig. 2 Visible absorption spectra of gold nanoparticles in the presence of difference concentrations of estrone关键词：可见光度法；纳米金；雌酮参考文献1 Frens G. Nat. Phys. Sci. 1973, 241: 20-222 Wang Z, Ma L. Coord. Chem. Rev. 2009, 253(1
3、1-12): 1607-16183 Kushnir MM, Rockwood AL, Yue B, Meikle AWM. ethods Mol. Biol. 2010, 603: 219-28Spectroscopy detection of estrogen using non-labeled gold nanoparticlesLing Yu1,2, Yanzheng Zhang3, Yadan Wang3, Yintang Zhang1, Maotian Xu1,3*1College of Chemistry and Chemical Engineering, Shangqiu Nor
4、mal University, Shangqiu 476000 2College of Chemistry and Chemical Engineering, Central South University, Changsha 410012 3College of Chemistry and Molecular Engineering, Zhengzhou University, Zhengzhou 450001The interaction of gold nanoparticles (AuNPs) with estrogen was studied by visible spectros
5、copy. Firstly, AuNPs were prepared through reduction of chloroauric acid by sodium citrate. The resultant nanoparticles were with good dispersity, and the mean particle diameter was about 35 nm. The absorption spectra had obvious red shift when AuNPs encountered estrone in Britton-Robinson pH 5.72 buffer. A good linear relation between absorption and logarithm concentration of estrone was found in the range of 0.0110x10-6molL-1 and with detection limit of 4.67x10-9molL-1. This assay was fast, low-cost and readily used to detect estrogen in milk powder and food.
