变温层积过程中华重楼种胚后熟生理生化的变化

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1、变温层积过程中华重楼种胚后熟生理生化的变化摘要通过观察和测定变温层积处理过程中华重楼种 子胚形态发育进程和体内SOD，POD，CAT，MDH，G-6-PDH 5种酶活性的变化，探究华重楼种子从休眠状态到解除休眠 状态过程的酶调控机制。结果显示，华重楼种胚在经过40 d 左右的暖温层积（181） C之后，进入快速发育阶段，同 期种子内在代谢活动显著加强，开始进入生理后熟阶段。在 层积第6080天种子基本完成形态后熟，尔后转入低温层 积（41） C后进一步促进了种子生理后熟。MDH和 G-6-PDH活性在种子层积第40天后分别发生显著的下降和 上升，通过调控种子呼吸代谢途径由三羧酸途径为主转为磷

2、酸戊糖途径为主，促进种子解除休眠。SOD，CAT活性在整 个层积过程中没有明显波动，但始终维持一定水平，以保证 种子活力。POD活性在进入低温层积阶段后显著上升，可能 参与调控了种子休眠解除的过程。关键词华重楼;种子休眠;胚后熟;SOD;CAT;POD;MDH;G-6-PDHPhysiological and biochemical change of Paris seed in after-ripeningduring variable temperature stratificationLI Zhao-ling1， TONG Kai1， YAN Shen1， YANGHuai, WANG

3、Qiao2, TANG Yong-binl,DENG Meng-shengl, TIAN Meng-liangl*(l.College of Agronomy, Sichuan Agricultural University, Chengdu 611130, China;2.Yaan Sanjiu Chinese Herbal Medicine Technology Industry Company, Yaan 625014, China )AbstractIn order to explore the dormancy physiological and biochemical mechan

4、ism of Paris seeds, the seed embryo growth courses, and the dynamic change of 5 enzymes, include SOD, POD, CAT, MDH, G-6-PDH were measured during variable temperature stratification. The results indicated that Paris seeds embryo grew quickly after 40 d in warm-stratification(18 1) C, at the meantime

5、 the metabolic activity was significantly strengthened. These facts showed that Paris seeds turned into physiological after-ripening process. After 60-80 d, the morphological embryo after-ripping process basically completed, and the following cold-stratification (4 1)C furthered Paris seed to finish

6、 physiological after-ripening. After 40 d, the activity of MDH decreased while G-6-PDH increased significantly. This showed that the main respiratory pathway of seed changed from TCA to PPP， which benifited breaking seed dormancy. In the whole period of stratification process, the activity variation

7、 of SOD and CAT was insignificantly and the activity of POD was enhanced significantly after shifting the seed in cold stratification process. This showed that SOD, CAT had no direct effects on breaking Paris seed dormancy but keeping the seed vigor, while the POD might involve in the process of Par

8、is seed dormancy breaking.Key wordsParis; seed dormancy; embryonic development;SOD; CAT; POD; MDH; G-6-PDHdoi： 10.4268/cjcmm20150411华重楼是百合科植物重楼属植物Paris polyphylla Smith var. chinensis (Franch.) Hara 的干燥根茎与滇重楼 P. polyphylla yunnanensis Smith var. (Franch.) Hand.-Mazz 并 列为中药“重楼”的正品药源，有清热解毒、消肿止痛、 凉肝定惊之效¹，是云南白药、季德 胜蛇药片、宫血宁、抗病毒颗粒等著名中成药的主要配方原 料。华重楼种子具有形态-生理休眠特性。自然情况下种子休 眠期长，发芽率低，导致华重楼资源再生相当困难。目前， 华重楼野生资源面临枯竭，市场供需矛盾非常突出。因此， 探究华重楼种子休眠机制，采取有效的处理措施，促使种子 尽早解除休眠，提高发芽率，是实现华重楼资源可持续发展 的有效途径之一。