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1、Primer of VectorPrimer(F)PACTT7IT3pACT2GAL4 AD|pACT2-RpAS2-1GAL4 BD|pAS2-1.RpB42ADpB42ADF|pB42ADRpBACPAK8BAC1|BAC2pBK-CMVT7T3PBS(SK/KS)/M13-M13F/T7|T3/M13RpBV220PBV220F|PBV220RpCAMBIA 1301(1300)P1P2pCAMBIA 2300M13R(-48)|M13F(-47)PCANTB5ES1/M13RS6pCAT3-enhancerRVP3|此载体无反向引物pcDNA3.0CMV-F/T7|SP6/BGHpcD
2、NA3.1T7BGHpcDNA4T7/CMV-F|BGHpcDNA6T7/CMV-F(J21025 在 T7 前面)|BGHsoc m mcc COL spp匸SP6/M13R匸oc coL z0 m匸oc coL scc coLSscopEGFP-N-3，匸c*5g牛d LUOco L s匸P匸cocoL2coL3匸匸pcDNAIIL&111 o111oEoo2OL g oCM CO oa c o aapDSRED-N1(KAN+)pEGFP-N-5PDSRED-N-RpECFP-CpEGFP-C-5pECFP-C-3pECFP-N1pEGFP-N-5pEGFP-N-3pEF/myc/ERp
3、EF-FpCDNA3.1RpEGFP-C(KAN+)pEGFP-C-5pEGFP-C-3pEGFP-N(KAN+)pEGFP-N-5pEGFP-N-3pENTR/D-TOPO(KAN+)M13FM13RpET-*(His)(KAN+)T7T7 TerpET22(a,b,c)(AMP+)T7T7 TerpET28,30,24,49(KAN+)T7T7 TerpET32(a,b,c)(AMP+)T7/S.tagT7 TerpET50(amp+)T7/s.tagT7TERpET44(AMP+)T7/s.tagT7 TerpEYFP-N1pEGFP-N-5pEGFP-N-3pEYFP-C1pEGFP-
4、C-5pEGFP-C-3pFLAG-CMVpFLAG-CMV -FpFLAG-CMV -RpGAD424GAL4 AD3ADpGADGHGAL4 AD3ADpGADT7-RecGAL4 AD/T73ADpGAPZa-Aa-FACTOR3AOXpGBKT7(Kana+)T73BDpGEM-T(-Easy)M13F/T7SP6/M13RpGEX-(*)TpGEX-4T-5pGEX-4T-3pGL2GLP1GLP2pGL3RVP3GLP2(RVP4)(没有特别说明用GLP2)PinpointTM VectorPinpoint PrimerSP6pIRESPIRES-F/T7T3/PIRES-R (J
5、40720)pIRES2-EGFPpIRES2-EGFP.P5pIRES2-EGFP.P3pLXSNpLXSN-FpLXSN-RpMAL-c2EMalE primerM13F(-47)pMAL-C2xP5P3pMAL-p2XMalE primerM13F(-47)PMD18-TM13R(-48)M13F(-47)PMD19-TM13F(-47)M13R(-48)pPIC9K(AMP+、ZEO+)5AOXa-factor3AOXpPICZa5AOX/PPICZa-F3AOXpPROEXHTAM13R(-48)无反向引物pQE30or40pQE30-FpQE-R:pRSETT7T7TERpSile
6、ncer3.1-H1 hygroM13F(-47)3.0REVpSILENCEV2.0-U6T72.0REVpSK01-TM13FM13RpSK-CMV(KAN+)T7T3pSP72T7SP6pSportlSP6/M13F(-47)T7/M13RpSUPERT7T3pTA2M13F/T7(优先使用 M13F)T3/M13RpT-AdvM13F /T7M13RpTARGET TMpTarget.F(在 T7 前面)/T7pTarget.RpTO-T7T7T7TERpTRC99a-cpTRC99C-FpTRC99C-RpTriPLEx25pTriPLEx2T7pTWIN-1T7T7TER（客户确认
7、再用）pUC18(19)/118(119)M13F(-47)M13R(-48)pUCm-TM13F/T7M13RpVAX1T7BGHpWSK29T7/M13F/M13F(-47)T3/M13R/M13R(-48)pXT7T7SP6pYES2T7pYes2.RpLEXApLEXA-FpLEXA-RpLNCXpLNCX-FpLNCX-RpRECEIVERCMV-F此载体无反向引物pSHUTTLE-CMVPSHUTTLE-CMV-FPSHUTTLE-CMV-RpSP64/65SP6此载体无反向引物pSTBlue-1T7SP6pT7Blue(R)T7M13F(-47)通用引物序列表引物名称序列(5-3
8、)M13RCAG GAA ACA GCT ATG ACCM13FTGT AAA ACG ACG GCC AGTM13F(-47)CGC CAG GGT TTT CCC AGT CAC GACM13R(-48)AGC GGA TAA CAA TTT CAC ACA GGAM13(-96)CCC TCA TAG TTA GCG TAA CGSP6ATT TAG GTG ACA CTA TAGT7TAA TAC GAC TCA CTA TAG GGT7 terminatorTGC TAG TTA TTG CTC AGC GGT3ATT AAC CCT CAC TAA AGG GApGEX-4T-5G
9、GG CTG GCA AGC CAC GTT TGG TGPGEX-4T-3CCG GGA GCT GCA TGT GTC AGA GGGLp1TGT ATC TTA TGG TAC TGT AAC TGGLp2CTT TAT GTT TTT GGC GTC TTC CARVp3CTA GCA AAA TAG GCT GTC CCRVp4GAC GAT AGT CAT GCC CCG CGpcDNA3.1RTAG AAG GCA CAG TCG AGGPinPoint primerCGT GAC GCG GTG CAG GGC GpCMV-FTCT AAA AGC TGC GGA ATT GT
10、pCMV-RTCCAAACTCATCAATGTATCPTRC99C-F:TTG CGC CGA CAT CAT AACPTRC99C-R:CTGCGTTCTGATTTAATCTGpCEP-F:AGA GCT CGT TTA GTG AAC CGEBV-R :GTG GTT TGT CCA AAC TCA TCpIRES2-EGFP.P5:GTA GGC GTG TAC GGT GGG AGpIRES2-EGFP.P3:AAC GCA CAC CGG CCT TAT TC3AD:AGA TGG TGC ACG ATG CAC AGCMV -FCGC AAA TGG GCG GTA GGC GTG
11、S1CAA CGT GAA AAA ATT ATT ATT CGCS6GTA AAT GAA TTT TCT GTA GTA GG5、A0X1GAC TGG TTC CAA TTG ACA AGC3、A0X1GCA AAT GGC ATT CTG ACA TCCa-FactorTAC TAT TGC CAG CAT TGC TGCGAL4 ADTAC CAC TAC AAT GGA TGpACT2-RGTGCACGATGCACAGTTGAApB42ADF:CCA GCC TCT TGC TGA GTG GAG ATGpB42ADR:AAG CCG ACA ACC TTG ATT GGA GpE
12、GFP-N-5:TGG GAG GTC TAT ATA AGC AGA GpEGFP-N-3:CGT CGC CGT CCA GCT CGA CCA GpEGFP-C-5:CAT GGT CCT GCT GGA GTT CGT GpEGFP-C-3:TAT GGC TGA TTA TGA TCA GTPBV220F:AAG AAG GGC AGC ATT CAA AGPBV220R:CTG CGT TCT GAT TTA ATC TGU6ATG GAC TAT CAT ATG CTT ACC GTA2.0rev primer:AGG CGA TTA AGT TGG GTA3.0rev:GAG
13、TTA GCT CAC TCA TTA GGCS.tagGAA CGC CAG CAC ATG GAC5TriplEx2CTC CGA GAT CTG GAC GAG CRVP4GAC GAT AGT CAT GCC CCG CGRVP3CTA GCA AAA TAG GCT GTC CCPQE30-RGTT CTG AGG TCA TTA CTG GPQE30-FTGA GCG GAT AAC AAT TTC ACpEF-FTCA AGC CTC AGA CAG TGG TTCpDSRED-N1-RTGA AGC GCA TGA ACT CCT TGpDsREDexpress-C1-FTCC CAC AAC GAG GAC TAC ACpCMV5RATT ATA GAG GA
