《通用引物序列》由会员分享,可在线阅读,更多相关《通用引物序列(1页珍藏版)》请在金锄头文库上搜索。
1、通用引物名称 序列(5-3)M13R: CAG GAA ACA GCT ATG ACCM13F: TGT AAA ACG ACG GCC AGTM13F(-47): CGC CAG GGT TTT CCC AGT CAC GAC RV-M GAGCGGATAACAATTTCACACAGG M13R(-48): AGC GGA TAA CAA TTT CAC ACA GGA M13(-96): CCC TCA TAG TTA GCG TAA CG SP6: ATT TAG GTG ACA CTA TAG T7: TAA TAC GAC TCA CTA TAG GGT7 terminator: T
2、GC TAG TTA TTG CTC AGC GGT3: ATT AAC CCT CAC TAA AGG GA pGEX-4T-5: GGG CTG GCA AGC CAC GTT TGG TG pGEX-4T-3: CCG GGA GCT GCA TGT GTC AGA GG GLp1: TGT ATC TTA TGG TAC TGT AAC TG GLp2: CTT TAT GTT TTT GGC GTC TTC CARVp3: CTA GCA AAA TAG GCT GTC CCRVp4: GAC GAT AGT CAT GCC CCG CG pcDNA3.1R: TAG AAG GCA
3、 CAG TCG AGG PinPoint primer: CGT GAC GCG GTG CAG GGC G pCMV-F: TCT AAA AGC TGC GGA ATT GT pCMV-R: TCCAAACTCATCAATGTATC pTRC99C-F: TTG CGC CGA CAT CAT AAC pTRC99C-R: CTGCGTTCTGATTTAATCTG pCEP-F: AGA GCT CGT TTA GTG AAC CG EBV-R : GTG GTT TGT CCA AAC TCA TC plRES2-EGFP.P5: GTA GGC GTG TAC GGT GGG AG
4、plRES2-EGFP.P3: AAC GCA CAC CGG CCT TAT TC 3AD: AGA TGG TGC ACG ATG CAC AGCMV -F: CGC AAA TGG GCG GTA GGC GTGS1: CAA CGT GAA AAA ATT ATT ATT CGCS6: GTA AAT GAA TTT TCT GTA GTA GG5AOX1: GAC TGG TTC CAA TTG ACA AGC 3AOX1: GCA AAT GGC ATT CTG ACA TCC a-Factor: TAC TAT TGC CAG CAT TGC TGC GAL4 AD: TAC C
5、AC TAC AAT GGA TG pACT2-R: GTGCACGATGCACAGTTGAA pB42ADF: CCA GCC TCT TGC TGA GTG GAG ATG pB42ADR: AAG CCG ACA ACC TTG ATT GGA G pEGFP-N-5: TGG GAG GTC TAT ATA AGC AGA G pEGFP-N-3: CGT CGC CGT CCA GCT CGA CCA G pEGFP-C-5: CAT GGT CCT GCT GGA GTT CGT G pEGFP-C-3 : TAT GGC TGA TTA TGA TCA GT PBV220F: A
6、AG AAG GGC AGC ATT CAA AGPBV220R: CTG CGT TCT GAT TTA ATC TG U6: ATG GAC TAT CAT ATG CTT ACC GTA 2.0rev primer: AGG CGA TTA AGT TGG GTA 3.0rev: GAG TTA GCT CAC TCA TTA GGC S.tag: GAA CGC CAG CAC ATG GAC 5TriplEx2: CTC CGA GAT CTG GAC GAG C RVP4: GAC GAT AGT CAT GCC CCG CG RVP3: CTA GCA AAA TAG GCT G
7、TC CC PQE30-R: GTT CTG AGG TCA TTA CTG G PQE30-F: TGA GCG GAT AAC AAT TTC AC pEF-F: TCA AGC CTC AGA CAG TGG TTC pDSRED-N1-R: TGA AGC GCA TGA ACT CCT TG pDsRED-express-C1-F: TCC CAC AAC GAG GAC TAC AC pCMV5R: ATT ATA GAG GAC ACC TAG TC pCMV5F: TTC CAA AAT GTC GTA ATA AC P5: TGC GTA CTG CGG TGA TCA AC
8、 P3: CTG CAA GGC GAT TAA GTT GG BAC2: ACG CAC AGA ATC TAG CGC TT BAC1: AAC CAT CTC GCA AAT AAA TA3BD: TAA GAG TCA CTT TAA AAT TTG TAT AC pTarget.F: CCA GGA TTT TCC CAG TCA C pTarget.R: GGC TTT ACA CTT TAT GCT TC T7(17base): ACA TCC ACT TTG CCT TTC TC pYes2.R: TCG GTT AGA GCG GAT GTG GAL4 BD: TCA TCG
9、 GAA GAG AGT AG pAS2-1.R: AAG AGT TAC TCA AGA ACA AGA A pFlag-CMV-F: GGT AGG CGT GTA CGG TGG pFlag-CMV-R: GCA CTG GAG TGG CAA CTT pIRES-F: CTT ACT GAC ATC CAC TTT GC pIRES.R: CAC TGC ATT CTA GTT GTG GT P1: CCA GGC TTT ACA CTT TAT GC P2: GCG ATT AAG TTG GGT AAC GC pLEXA-F: CGT CAG CAG AGC TTC ACC AT
10、pLEXA-R: TAA AAC CTA AGA GTC ACT TT pLNCX-F: AGC TCG TTT AGT GAA CCG TCA GAT CG pLNCX-R: ACC TAC AGG TGG GGT CTT TCA TTC CC pLXSN-F: CTT GAA CCT CCT CGT TCG AC pLXSN-R: GTT GCT GAC TAA TTG AGA TG pSHUTTLE-CMV-F: GGT CTA TAT AAG CAG AGG TG pSHUTTLE-CMV-R: GTG GTA TGG CTG ATT ATG ATC AG MalE Primer: GGT CGT CAG ACT GTC GAT GAA GCC