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1、华中科技大学硕士学位论文本研究的创新之处本文以卵巢切除大鼠为模型，从一般情况，骨组织水平，骨整体水平，血清生化水平对卵巢切除对骨代谢的影响表现进行了较为系统的研究。III华中科技大学硕士学位论文Study on ovariectomy affects the bone metabolism of ratsMaster Candidate: Linyuan ZhouSupervisor: Prof. Nianhong YangAbstractAfter menopause, the function of ovary is decreased, the secretion of estrogen
2、 isdescended. The short of estrogen affects bone metabolism through a variety of ways.It is controversial about the representation that the estrogen affects the bonemetabolism, such as bone turnover, bone calcium, bone mineral density and bonestrength.Futhermore, it lacks a systematic research. Ther
3、efore, ovariectomized ratwas the object of our experiment, used it to study the representation of the estrogenaffects the bone metabolism.Objective: To study the mechanism and the influence of ovariectomy on the bodyweight, visceral fat, bone mineral density and bone strength.Methods: Forty five fem
4、ale Sprague Dawley rats, 2558.6g, were randomly dividedinto 3 groups: sham operation group(Sham,n=15), ovariectomized group (Ovx,n=15),ovariectomized plus estrogen group (Ovx+ E2, n=15). Ovariectomized (Ovx) andovariectomized plus estrogen group (Ovx + E2) were removed bilateral ovaries,thesham oper
5、ation group underwent open surgery, not removed bilateral ovaries. Twoweeks after the operation,ovariectomized plus estrogen group (Ovx + E2)was givengavage complement estradiol valerate suspension with amount of 1ml/100g*d, thesuspension concentration was 0.08 mg/ml, ovariectomized group and sham o
6、perationgroup was given daily gavage complement saline with amount of 1ml/100g. weighedthe rats every week. Feeding three months after surgery, the rats were sacrificed bydecapitation, collected the serum and bilateral fermur, measured the weight of visceralfat， the left femurs bone calcium, bone mi
7、neral content, area, bone mineral density ,weight, length, the right femurs bone strength, the activity of serous alkalinephosphatase, tartrate resistant acid phosphatase and osteocalcin.IV华中科技大学硕士学位论文Results: After operated one week, ovariectomized groups body weight was higherthan the sham operati
8、on group, and kept this trend until the feeding end; the secondweek after operation, ovariectomized plus estrogen groups weight started to descend,and the weight was lower than the ovariectomized group at the week 13 after theoperation. The weight of visceral fat in ovariectomized group was higher t
9、han thesham operation group and the ovariectomized plus estrogen group; the ratio of thevisceral fat and body weight in the ovariectomized plus estrogen group was lowerthan the ovariectomized group. The left femurs bone calcium in the ovariectomizedgroup was lower than the sham operation group and t
10、he ovariectomized plus estrogengroup; there was no difference between three groups about the left femurs bonemineral content; the ovariectomized group and the ovariectomized plus estrogengroups distal of the left femurs bone mineral density were lower than the shamoperation group, the whole and midd
11、le of left femur bone mineral density betweenthree groups were no differences. The whole area of the left femur in theovariectomized group was higher than the sham operation group and theovariectomized plus estrogen group. There were no differences between three groupsabout the left femurs weight, l
12、ength and right femurs bone strength. Both of theactivity of the alkaline phosphatase andtartrate resistant acid phosphatase inovariectomized group were higher than the sham operation group; the level of serumosteocalcin in the ovariectomized group and ovariectomized plus estrogen group werehigher t
13、han the sham operation group.Conlusions: In our experimental conditions, after ovariectomy:increased the ratsbody weight and accumulated the visceral fat, the estrogen suppressed the rise of thebody weight and the visceral fats accumulation;decreased the femurs bonecalcium, the estrogen suppressed t
14、he loss of bone calcium;decreased the distal offemurs bone mineral density, the cancellous bone was more sensitive to theovariectomy;not decreased the femurs bone strength;caused a high level ofturnover, bone absorption superior to bone formation, the estrogen could suppress theV华中科技大学硕士学位论文bone loss.Keywords: ovariectomy; estrogen; female Sprague Dawley rat; bone metabolism;bone mineral densityVI华中科技大学硕士学位论文前言大部分女性开始快速的骨丢失是和绝经期有关的，这被认为是由于卵巢功能的
