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1、（7.72%0.53%）的饲料，分别是 0%，30%，50%，70%和 90%鱼粉蛋白水平替代组（下文分别用 D0、D30、D50、D70和 D90来表示）。实验结果表明，各处理组的成活率和特定生长率都没有显著性差异（P0.05），增重最多的是 D70处理组，特定生长率是 6.540.02%/d；饲料系数，蛋白质效率各组别之间均无显著差异（P0.05），其中 D90处理组有最低的饲料系数和最高的蛋白质效率，分别是 1.010.01和 2.670.01；对虾的体成分组成并没有随着替代水平的上升而显著变化（P0.05）；随着复合动物蛋白源替代水平的升高，对虾的超氧化物歧化酶SOD和过氧化氢酶 CA
2、T活性呈现先增强后减弱的趋势，在 D50处理组都达到最高水平；溶菌酶活性则是随着替代水平的升高呈现先减弱后增强的趋势，与 SOD和 CAT的变化趋势相反。研究表明：复合动物蛋白源可以替代饲料中 90%的鱼粉蛋白而不影响凡纳滨对虾的生长性能和饲料利用情况，并且可以提升凡纳滨对虾超氧化物歧化酶和过氧化氢酶活力。3.以初重为（0.300.01）g的凡纳滨对虾为实验对象，在室内流水养殖系统（养殖桶规格：250L）中进行为期 8周的摄食生长实验，研究在饲料中添加黄粉虫（Tenebrio molitor）虫粉对凡纳滨对虾生长性能、体成分组成和非特异性免疫能力的影响。制作四组等蛋白质（37.81%0.14%
3、）、等脂肪（8.81%0.23%）的饲料，分别是对照组、1.5%、3.0%和 4.5%添加组。实验结果表明：3.0%添加组和4.5%添加组的成活率显著性低于对照组（ P0.05）；特定生长率最大的是对照组，为 6.480.02%/d，4.5%添加组显著低于对照组（P0.05）；4.5%替代组的饲料系数和蛋白质效率显著低于对照组（P0.05）；不同处理组凡纳滨对虾的血清的超氧化物歧化酶 SOD活性没有显著差异（P0.05）；过氧化氢酶活性 4.5%添加组显著高于其余各组（P0.05). Feed converse rate FCR and protein efficiencyrate PER w
4、ere slightly better in D20 than other diets, FCR was not affected by any ofthe test diets. D100 and D40 showed the highest superoxide dismutas SOD activity inserum, 43.622.53U/mg、 40.411.67U/mg respectively, there is no significantdifference among other diets, the catalase CAT showed the max activit
5、y in D20% andD80%, while D60% have the lowest activity, activity of the phenoloxidase PO werehigher in D40%, D60% and D80%, no significant differences were observed betweenthe rest.2. In present study, it evaluated the growth and nonspecific immune characteristicsof the Pacific white shrimp in respo
6、nse to the replacement of fish meal in shrimp dietwith increasing levels of mixed animal protein(Poultry by-product meal, blood meal,further meal, with ratio 6.5 : 2 : 1.5), shrimp with initial weight 0.300.01g werestocked into 250L indoor experimental system and reared in a 8-week period. Fiveisoni
7、trogenous (37.81%0.14%) and isolipdic (8.81%0.23%)IIIexperimental dietswere formulated with 0%, 30%, 50 %, 70 % , 90% MAP relacement of fish mealrespectively. There is no significant difference between the groups in survival rate, SGR,FCR and PER(P0.05), the shrimp fed on the 70% MAP inclusion exhib
8、ited the best ingrowth, while the D90 showed the lowest growth. Carcass composition differed slightlyamong groups. Activity of superoxide dismutase SOD and the catalase CAT showed thedown then up trend with the increasing levels of MAP inclusion, D50 showed thehighest activity; lysozyme activity had
9、 a up then down trend with MAP replacement,while D50 had the lowest activity, contrary to the SOD and CAT.3. The study of nutritional value of Yellow mealworm (Tenebrio molitor) meal(YMM) as an addictive in diet was conducted, Pacific white shrimp (Litopenaeusvannamei) initial weight (0.300.01g) wer
10、e stocked into 250L indoor experimentalsystem and reared in a 8-week period to evaluate the growth response, carcasscomposition and nonspecific immune characteristics of the shrimp. Four isonitrogenous(37.81%0.14%) and isolipdic (8.81%0.23%) experimental diets were formulated with0, 1.5%, 3.0%, 4.5%
11、 YMM respectively. The survival rate of shrimp fed the dietscontained 3.0% and 4.5% YMM were significantly lower than that fed on the controldiet (P0.05); the control diet group exhibited the best in growth, food converse rateand the protein efficiency rate, significantly higher than that of 4.5% inclusion of YMM(P0.05); no significant difference was observed in c
