《化学缺氧以及外源性血管内皮生长因子对脐静脉内皮细胞可溶性血管内皮生长因子受体-1mrna表达影响的研究论文》由会员分享，可在线阅读，更多相关《化学缺氧以及外源性血管内皮生长因子对脐静脉内皮细胞可溶性血管内皮生长因子受体-1mrna表达影响的研究论文（36页珍藏版）》请在金锄头文库上搜索。
1、华中科技大学硕士学位论文用呈现剂量依赖关系；3. VEGF在拮抗 CoCl2诱导脐静脉内皮细胞缺氧损伤时，细胞内 sFlt-1mRNA表达强度下降，其作用呈剂量依赖关系。关键词：妊娠高血压疾病；人脐静脉内皮细胞；可溶性血管内皮生长因子受体-1；血管内皮生长因子3华中科技大学硕士学位论文Study on the effects of chemical hypoxia induced by cobaltchloride and extraneous VEGF on cell injury, proliferation andthe expression of soluble vascular en
2、dothelial growth factorreceptor-1 mRNA of human umbilical vein endothelial cells.AbstractPart 1Chemical hypoxia effects of cobalt chloride on cell injury,proliferation and the expression ofsoluble vascular endothelial growthfactor receptor-1 mRNA of human umbilical vein endothelial cells.Objective:
3、investigate the chemical hypoxia effect of cobalt chloride on the cell injury,proliferation, and the expression of mRNA of soluble vascular endothelial growth factorreceptor-1(sFlt-1) of human umbilical vein endothelial cells.Methods: cell injury was evaluated by cell viability (MTT assay), lacticde
4、hydrogenase (LDH) released from all group HUVECs into the medium were measuredby using LDH kits, and the expression of sFlt-1 of all group was detected by using thequantitative reverse transcriptase-polymerase chain reaction (RT-PCR).Results: cobalt chloride could injury HUVECs, induced the expressi
5、on of mRNA ofsoluble vascular endothelial growth factor receptor-1(sFlt-1), and there was a timedependent manner.Conclusion: HUVECs were injured induced by COCl2, proliferative ability waslessened and the expression of sFlt-1 mRNA was up-regulated, the expression of sFlt-1mRNA of HUVECs may play an
6、important role in the development of hypertensivedisorder complicating pregnancy.Key words: Hypertensive disorders complicating pregnancy; Human umbilical veinendothelial cell; soluble fms-like kinas receptor-1; chemical Hypoxia;4华中科技大学硕士学位论文Part 2 The investigation on the agonistic effect of extran
7、eousVEGF on the injured Human umbilical vein endothelial cellsinduced by CoCl2Objective: to investigate the effect of extraneous VEGF on the expression of mRNAof soluble vascular endothelial growth factor receptor-1(sFlt-1) of human umbilical veinendothelial cells induced by chemical hypoxia with Co
8、Cl2Methods：The cells had been cultured under serum starvation condition for 12 hour,which were randomly divided into the following groups:normal control group (cells were incubated under normal condition);hypoxic group (The cells were incubated under condition of the 21 umol concentration of CoCl2fo
9、r 12hour, 24hour and36hour respectively);hypoxic and VEGF group (given the 100ng/L, 200ng/L, 300ng/L concentration ofVEGF to above group and kept the incubational time as those);VEGF control group.( given the 100ng/uL,200ng/uL,300ng/uL concentration ofVEGF to cells and incubated under normal conditi
10、ons for 12hour, 24hour and36hourrespectively);Detect the expression of sFlt-1 of all group by using the quantitative reversetranscriptase-polymerase chain reaction(RT-PCR), lactic dehydrogenase (LDH) releasedfrom all group HUVECs into the medium were measured by using LDH kits，cell injurywas evaluat
11、ed by cell viability (MTT assay).Results：1. Incubation of endothelial cells with CoCl2 for12h, 24h, 36h, the mount of LDH andMTT were increased accompanying with the hypoxia time and had the same up tendencyby the hypoxia time, these conditions elicited the increase in the extent of sFlt-1 mRNA5华中科技
12、大学硕士学位论文and there was a significant difference of its among every groups(P0.01);2. The expression of sFlt-1 mRNA was lessened and had a concentration-dependentmanner, the mount of LDH and MTT were decreased in concomitant with the differentconcentration of VEGF, its tendency was time-and concentrati
13、on-dependent manner.3.sFlt-1 mRNA expression levels, the mount of LDH and the number of death cells hadcorrelations with each other respectively.Conclusion：1. HUVECs induced by CoCl2 had these manifestations: the release of LDH and thenumber of death cell elevated, the expression of sFlt-1 mRNA was
14、up-regulated;2.3.Extraneous VEGF had the protective effects on the injury cells induced by CoCl2.The extraneous VEGF not only had the anti-hypoxic effect but also inhibited theexpression level of sFlt-1 mRNA.Key words： Hypertensive disorders complicating pregnancy; Human umbilical veinendothelial cell; soluble fms-like kinas receptor-1; vascular endothelial growth factor.6独创性声明本人声明所呈交的学位论文是我个人在导师指导下进行的研究工作及取得的研究成果。尽我所知，除文中已经标明引用的内容外，本论文不包含任何其他个人或集体已经发表或撰写过的研究成果。对本文的研究做出贡献的个人
