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1、Chapter 24,(HHV),Human Herpes Virus,人类疱疹病毒,Outline,Classification of herpesviruses Characteristics of herpesviruses Quick diagnosis of herpesviruses,1.Classification,(P310 Table 24-1),-herpesviruses,HHV-1(HSV-1) HHV-2(HSV-2) HHV-3(VZV), -herpesviruses, -herpesviruses,HHV-4(EBV) HHV-8,HHV-5(CMV) HHV-
2、6 HHV-7, Shape and structure,Glycoprotein spike,Linear ds-DNA,Cubic nucleocapsid,Envelope,120-130nm in diameter,162 capsomeres,2.Characteristics,tegument, Cultivation,- replicate in intranuclear of human diploid cells and produce cytopathic effect,typical CPE: intranuclear acidophilic inclusion body
3、 cell fusion form multinuclear giant cells,核内嗜酸性包涵体,(except EBV),细胞融合形成多核巨细胞, Pathogenicity, transmission, infectious mode of virus on host cells,proliferative latent infection infection,activate, common infectious types,(by many different routes),*subclinical infection *primary infection and latent
4、 infection *congenital infection *integrative infection,人普遍易感,感染后大多无明显症状,Finding inclusion body Detection of viral Ag and specific Ab Detection of viral DNA,3.Quick diagnosis,(CMV),(HSV,CMV,EBV),1.Serotype :HSV-1 and HSV-2 2.Pathogenicity, by direct contact by sexual contact,病人和健康带毒者,HSV-1:主要是直接密切接触
5、, 也有性接触传播 HSV-2:性接触传播为主,Herpes Simplex Virus, Source of infection Route of transmission HSV-infection, HSV-infection, Primary infection, Latent and recurrent infection, Congenital and newborn infection, Associated with cancer,primary infection,(anew),recurrent infection,(reactivation of latent virus
6、),(HSV-1, HSV-2),(site of latency),复发性局部疱疹,HSV1-唇癌 HSV2-宫颈癌,大多无明显症状或出现局部疱疹,latent infection,HSV-1-三叉神经节 HSV-2-骶神经节,Coldsore or fever blisters (唇疱疹或热性疱疹), Single serotype, Route of transmission, Primary infection,Recurrent infection, varicella (chidren) pneumonia (adults),- zoster(adults),单侧成簇性水疱群沿神经
7、支配的皮肤排列呈带状，伴有神经痛,孕妇患水痘易致胎儿畸形、流产或死产,通过飞沫和直接接触传播,Varicella-Zoster Virus,(VZV),1.Species-specific,2.Cultivation,3.Pathogenicity,(slow growth, typical CPE),细胞肿胀、核变大,形成巨大细胞,核内有“猫头鹰眼” 的嗜酸性包涵体,HCMV只能感染人,只能 在人成纤维细胞中增殖, Primary infection,（usually asymptomatic infection）,latent infection (site of latent), Rou
8、te of transmission,(transmitted by a variety of route),Cytomegalovirus,(CMV),Vertical transmission,*隐性感染 *巨细胞包涵体病(CID) *流产、早产或死胎 *先天性畸形/出生后异常,*by transfusion post-transfusion mono-nucleosis and hepatitis *by close contact and sexual contact *patients with impaired immune function activation of laten
9、t virus,congenital infection perinatal infection, Horizontal transmission, Cell transformation & oncogenic potential,致癌潜能,(耳聋和智力障碍),肺炎/视网膜炎/食管炎等,经产道感染(围生期感染),1.Specific antigen of EBV,(EBNA and LMP),( VCA, EA and MA), Expressive antigen in lentant infection, Antigen associated with proliferative inf
10、ection,中和抗原,Epstein-Barr Virus,(EBV),2. Infectious mode in B cell,Proliferative infection Non-proliferative infection,proliferative infection,lentant infection malignant transformation,细胞溶解死亡,恶性肿瘤细胞,（嗜B细胞的人疱疹病毒，主要侵犯B细胞）,activate,3. Pathogenicity, Route of transmission, EBV & human disease, primary i
11、nfection,*asymptomatic *infectious mononucleosis (puberty), diseases associated with EBV- infection,*Burkitt lymphoma (BL) *nasopharyngeal carcinoma(鼻咽癌),主要通过唾液传播,infectious mononucleosis,* positive-heterophil antibody (80%90%),* positive EBV-specific antibody,abnormal lymphocyte,（传染性单核细胞增多症）,异嗜性抗体阳性,EB病毒的特异性抗体阳性,* abnormal lymphocytes(10%20%),与CMV感染引起的输血后传染性单核细胞增多症不同(两种抗体均阴性),
