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1、The effect of ApoE4 on Fear Conditioning,Michael A. Manoharan Valerio Dr. Summer Acevedos lab Ponce School of Medicine,ApoE,Gene located on chromosome 19q13.2 299 amino acid residue (34 kDa protein) Produced by astrocyte cells and is expressed in the brain Assists in the regulation of lipid metaboli
2、sm Major alleles: apoE2, apoE3, apoE4 ”Exon 4” Risk factor: apoE2 Cardiovascular disease Alzheimer disease,apoE4 Diabetes/Hypertension Alzheimer disease,http:/en.wikipedia.org/wiki/File:Gfapastr5.jpg,http:/en.wikipedia.org/wiki/File:PBB_Protein_APOE.jpg,http:/ghr.nlm.nih.gov/dynamicImages/chromomap/
3、apoe.jpeg,Methods,3,2,1,4,II. PCR,I. DNA extraction,CfoI cut sites,30 16 19 72 18 48 35,5-F6,3-F4,C112R,R158C,IV. RFLP Analysis,III. Digest,PCR,95 C,65C,72C,ApoE Genotyping,3,2,4,30 16 91 18 83,30 16 19 72 18 48 35,30 16 91 18 48 35,C112R,R158C,Alleles,ApoE RFLPs,M1 2/2 3/3 4/4 2/3 2/4 3/4 M2,100bp,
4、75,50,100bp,4 % agarose gel,50,Photos,Fear Conditioning,This technique is used to study the acquisition and extinction of fear. The most common technique of Fear conditioning is the Pavlovian conditioning. The technique used was Associative Learning which is pairing an unconditional stimulus and a c
5、ondition stimulus so the organism can learn fear. The Unconditional Stimulus is something that naturally provokes a response. The Conditioned Stimulus is something that paired with the unconditional stimulus will get a response because naturally it will not create a response.,Fear Conditioning,Milad
6、 2006 at all,ApoE4 effect Fear Recall,*,* F = 6.70 p0.02,N=20,N=14,Acknowledgements,Collaborators: Summer F. Acevedo, PhD Tirtsa Porrata-Doria Jaime Matta, PhD Julie Dutil, PhD Project Funded by: Moffit Cancer Center Grant (60-14599-01-01-54) through Dr. Acevedo, R01 HD050559 NIH-MBRS Program grant S06 GM008239-20 to PSM through Dr. J. Matta RCMI High School Supplement Special thanks to the PSM Molecular Biology Core Lab (NCRR-G12RR003050-24) for their assistance and support on the development of this investigation.,
