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1、Wednesday & Thursday Lab Sections results (combined 47 students),ALL but four repeated gram stains were done successfully. Scores for Lab 1 have been updated.ALL but one aseptic transfers (broths and slants) were done successfully (2 points).XX/47 streak plates were successful (3 points). X had one
2、of the two colonies separated (2 points) can repeat in an upcoming lab; X had no colonies separated (1 point) can repeat in an upcoming lab.Agar plate cultures with “unique” characteristics - Be sure to record these unique characteristics in your lab notebook (will be scored in lab next week 2 point
3、s possible).Selective & differential media - Blood agar, EMB, MacConkey, Mannitol Salt not done this week (media not available). Will be done in next weeks (Week 3) lab (plenty of time to add this in next week). So be sure to bring your Week 2 lab procedure with you.,A NEW SUPERBACTERIA, IMMUNE TO M
4、OST ANTIBIOTICS, FOUND SPREADING FAST,What is an antibiotic? http:/ antibacterial substances produced by microorganisms Semi-synthetic derivatives of natural antibiotics What are some sources of natural antibiotics? Molds e.g., penicillin G from Penicillium notatum Bacteria e.g. tetracycline from St
5、reptomyces speciesSome examples of semi-synthetic antibiotics Penicillin “family” - methicillin, ampicillin, oxycillin Doxycycline is a semi-synthetic tetracycline,TWO Clicker question will be asked at the start of class 30 seconds to answer each question,1. Bacteria have many characteristics that c
6、ontribute to the ability to initiate or cause infectious diseases. For example, certain bacteria have cell components that enable those cells to attach to human body cells, which can be a necessary pre-requisite to an eventual pathology. Which ONE LETTER gives examples of TWO bacterial cell componen
7、ts that can be involved in attachment to human cells? (Select ONE letter as your answer)2. Autoclaving and its consumer equivalent, pressure cooking, are methods designed to destroy which one of the following components of bacterial cells? Hint: these components are most often found in members of th
8、e two genera, Clostridium and Bacillus.HANDOUT of modified ppt slide at ends of each classroom bench,Bacteria have many characteristics that contribute to the ability to initiate or cause infectious diseases. For example, certain bacteria have cell components that enable those cells to attach to hum
9、an body cells, which can be a necessary pre-requisite to an eventual pathology. Which ONE LETTER gives examples of TWO bacterial cell components that can be involved in attachment to human cells? (Select ONE letter as your answer),A. capsule and Lac permeaseB. capsule and fimbriaC. peptidoglycan and
10、 periplasmic spaceD. capsule and nutrient-binding casette,Autoclaving and its consumer equivalent, pressure cooking, are methods designed to destroy which one of the following components of bacterial cells? Hint: these components are most often found in members of the two genera, Clostridium and Bac
11、illus.,A. Endotoxins (lipid portions of bacterial LPS)B. capsules (either carbohydrate or protein)C. endosporesD. MRSA,Start of September 16 Discussion,A NEW SUPERBACTERIA, IMMUNE TO MOST ANTIBIOTICS, FOUND SPREADING FAST,What is an antibiotic? http:/ antibacterial substances produced by microorgani
12、sms Semi-synthetic derivatives of natural antibiotics What are some sources of natural antibiotics? Molds e.g., penicillin G from Penicillium notatum Bacteria e.g. tetracycline from Streptomyces speciesSome examples of semi-synthetic antibiotics Penicillin “family” - methicillin, ampicillin, oxycill
13、in Doxycycline is a semi-synthetic tetracycline,What are some typical “targets” of some common antibiotics?,Cell walls peptidoglycans - penicillin Ribosomes - tetracycline DNA replication enzymes (e.g., DNA gyrase) ciprofloxacin (“cipro”) What is DNA gyrase? Unwinds dsDNA to allow access to single-s
14、tranded template for DNA replication DNA gyrase is not found in humans (analog is DNA helicase in humans)How do bacteria resist antibiotics?,Bonus Clicker question “have I seen this one before?” Penicillin, methicillin, and vancomycin are antibiotics all of which interfere with or destroy bacterial
15、peptidoglycans. Therefore the “target” of these antibiotics is which one of the following structures of bacterial cells?,A. lipopolysaccharides (LPS)B. cell wallC. cell membraneD. teichoic acids,Cell wall and antibiotics,Cell wall is an important antibiotic target Todors Online Textbook of Bacteriol
16、ogy: Antimicrobial Agents in the Treatment of Infectious Disease (page 3) http:/ (scroll down to “Cell wall synthesis inhibitors”),Structure and Function,Cell Wall Todors Online Textbook of Bacteriology: Structure and Function of Bacterial cells (page 5 only) Http:/ - imparts some rigidity Sugar backbone - NAG & NAMA (Human lysozyme cleaves (1,4) bond) Amino acid cross links adds rigidity Peptidoglycan layer thickness,