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1、Immuno-Seq: exploring the diversity of immune systemJiankui He South University of Science and Technology of China(SUSTC), Shenzhen, ChinaThe diversity of Invaders . . . Bacteria Viruses parasites such as fungi, protista, 5(171):171接受流感疫苗后Immuno-Seq Immuno-Seq技术可以观察和分析T细胞和 B细胞，达到前所未有的深度和特异性。 在疾病监控，抗
2、体生产，疫苗研究，健康 体检等领域有广泛的应用。现在临床上有许多种检测可以监控你的健康状况， 但是却没有一种被广泛接受的方法可以检测你免疫系统的各个方面。 我们怎么可以得知一个人的免疫系统是很强健还是很虚弱？ 在人们受哮喘，过敏，关节炎折磨时，如何量化免疫系统的错误？ 斯坦福大学的一个研究团队在开发这样的检测方面迈出了第一步， 他们的方法是：给免疫系统“拍照”。There are numerous tests to gauge the health of your heart. But no such widely accepted test exists for the many parts
3、 of the immune system. How can we tell if the immune system is strong or weak? Or quantify how badly its malfunctioning when we suffer from asthma, allergies, or arthritis?-Francis Collins, NIH Director2013年2月13日Immune response to flu vaccineDay 0Day 7Day 28Flu Vaccine: TIV & LAIVPlasma blasts Day 7
4、Collect 5ml bloodExperiment flowBlood samplePatient TissuesSingle cell SuspensionPBMCTCD8Genomic DNARNAAmplify the library by Multiplex PCRIllumina Hiseq 2000Alignment and ClusteringBroad VDJ gene segment usage patternVDJA snapshot of immune systemDay 7, after vaccination. Elderly subjectWe amplifie
5、d antibody sequences from single sorted plasma B cell . We then expressed monoclonal antibodies according to these sequences and verified their binding to each of the three virus strains in the vaccineImmune reperotire is a dynamics systemDay 0Day 7Day 28Vaccine specific antibody lineage 13% of tota
6、l sequencesVaccine specific antibody lineage 0.01% of total sequencesElderly subjectPlasma blasts Day 7Reduced antibody diversity in elderlyAge 8-17Age 18-32Age 70-100MutationEach data point represent a lineageComparison of 3 subjectsSequences in a lineageReduced antibody diversity in elderly70100 y
7、ear old volunteers had a significantly less diverse collection of antibodies than the younger volunteers. That is, as people aged, their immune system had a more limited repertoire of antibodies to offerLab MembersPostdoc: Zhoufang LiTechnician: Luwen Ning Meng ZhangStudents: Jiajie Guo Yin Tong Yin
8、g Xu Peng Li Yao Chen Ronghai ChengCollaborators: Pinghua Liu (Boston U), Hengye Man (Boston U) Zhigang Liu (Shenzhen U), Zhanhui Wang (SMU), Zhiming Cai (2nd Hospital of Shenzhen), Xiao Liu (BGI) Xiao PingChen (CAS), Hua Zhang (SMU)Lab web: www.sustc-免疫组库测序服务提供商深圳市瀚海基因生物科技有限公司 专注于提供人，小鼠，猴子等的TCR/BCR免疫组库测序 网址：
