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1、活细胞旋转共聚焦成像1-2%1-2%NipkowNipkow disc with disc with pinholespinholesNipkow Spinning Disk70%70%Collector Collector disc withdisc with microlensesmicrolensesNipkow Nipkow disc with disc with pinholespinholesDual Spinning DiskConfocal Imaging Conjugate focal planesPinhole array scanningPinhole array sca
2、nningSingle point scanningSingle point scanning e.g. galvo scannerse.g. galvo scannersPoint Scanning vs. Dual Spinning Disk 5D Multi-field Time lapse3 fields from a 5D, 24h time lapse, 10 minute intervals, 33Z sections. HeLa cells expressing H2B GFP a nucleosome organizing histone. Field 1Field 1Field 2Field 2Field 3Field 3Total Internal Reflection Fluorescence ( TIRF )8HIV TIRFMicropoint9Tuneable to over 20 wavelengthsAblate/cutDNA damageUncage (e.g. calcium)Bleach/FRAPPhotoactivate
