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1、近期发表的RetroNectinTM相关的部分论文 1. Marion G Ott. et al. Correction of X-linked chronic granulomatous disease by gene therapy, augmented by insertional activation of MDS1-EVI1, PRDM16 or SETBP1. Nature Medicine 2006 12, 401 409 Context: .School) and K. Cichutek (Paul-Ehrlich-Institute) for the gift of mate
2、rials and discussions during this work. RetroNectin (CH-296) was provided by Takara Bio Inc. This work was supported by the Swiss National Science Foundation.2. Marina Scheller. et al. Hematopoietic stem cell and multilineage defects generated by constitutive -catenin activation. Nature Immunology 2
3、006 7, 1037 1047 Context: .of 1:1 in the presence of 8 g/ml of polybrene and a cytokine cocktail, were plated onto CH296-coated plates (Retronectin; Takara Shuzo), were inoculated by centrifugation for 90 min at 2,200 r.p.m. in a Heraeus 8074 rotor and were. 3. Ivan Bilic. et al. Negative regulation
4、 of CD8 expression via Cd8 enhancermediated recruitment of the zinc finger protein MAZR. Nature Immunology 2006 7, 392 400 Context: .bone marrow cells were transferred to a 10-cm nontissue-culture-treated plate (Sterilin) precoated with RetroNectin (Takara). Infections were done according to the man
5、ufacturers instructions by incubation for two to three rounds. 4. Alex H Chang. et al. Stem cellderived erythroid cells mediate long-term systemic protein delivery. Nature Biotechnology 2006 24, 1017 1021 Context: .Gy with 4-h interval) on the day of transplantation. Bone marrow cells were transduce
6、d in serum-free medium on RetroNectin-coated 6-well plate (15 g/ml, TAKARA Shuzo) for 8 h. Bone marrow cells (5 105-1 106 per mouse) were then.5. Marjorie A Robbins. et al. Stable expression of shRNAs in human CD34+ progenitor cells can avoid induction of interferon responses to siRNAs in vitro. Nat
7、ure Biotechnology 2006 24, 566 571 Context: .vector stock was adjusted to a multiplicity of infection (MOI) of 40 in 200 l culture medium and loaded onto RetroNectin-coated 24-well plate (Takara Mirus). After incubation at 32 C for 4 h, the vector supernatant was removed and. 6. David C. Dorn. et al
8、. Hematopoiesis Controlled by Distinct TIF1 and Smad4 Branches of the TGF Pathway. Cell 2006 125: 929-941 7. Selda Samakoglu. et al. A genetic strategy to treat sickle cell anemia by coregulating globin transgene expression and RNA interference. Nature Biotechnology 2006 24, 89 94 8. Vladimir Jankov
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