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1、 I HMG POF HMG 1. 0.00IU/mL 0.15IU/mL 0.30IU/mL 0.60IU/mL 12h 24h 48h VEGF 2. 0.00IU/mL 0.15IU/mL 0.30IU/mL 0.60IU/mL 3h 24h 36h 72h 1 HMG 1. HMG 406.9061.53 133.382.26 P0.05 CTG Bcl-2 48.0819.84 P0.001 Bax CTG 34.61 17.00 FTG P 0.05 CTG Caspase-3 54.41 44.10 P0.05 Bcl-2 Bax Caspase-3 HMG 0.60 -CTG
2、FTG CTG 6.830.75 P0.05 1 HMG 0.15 -CTG P0.05 HMG 0.60 -CTG 6.912.51 HMG 0.15 -CTG 9.363.02 FTG 8.042.85 P0.05 HMG VEGF HMG HMG 0.15IU/mL 0.30IU/mL 0.60IU/mL III Study the Effect of the Transient Intervention of HMG on the Result of Ovary Transplantation in Mice ABSTRACT The cryopreservation and tran
3、splantation of ovarian tissue, as a way of conservation and recovery the fertility of the female, give hope to those who suffer from endocrine dyscrasia and fertility deprivation as a result of POF or ovriectomy or congenital ovarian dysgenesis. The method of ovarian cortex transplanted after cryopr
4、eserved without vascular anastomosis is easier, more economic than that of ovary organs, and the former is easier to be performed and can avoid the forming of thrombus which much often occurs after the vascular anastomosis. However, the ischemic hypoxia and ischemicreperfu sion injury after the oper
5、ation often cause great loss of primordial follicles, which results in low survival rate and worse function and shorter life span of the transplanted ovary. Some researches believe that the secretion of Gn after the ovary transplanted is helpful to the revascularization of the graft. Purpose This re
6、search is to explor not only the influence of the revascu- larization caused by the intervention of HMG during the time of in vitro and transplanting but also some mechanisms.Methods Observe the VEGF expression in ovarian tissue by immunohistochemical method after cultured the hemi-ovaries of juvene
7、scent mice in the culturing medium that contain HMG 0.00IU/mL, 0.15IU/mL, 0.30IU/mL and 0.60IU/mL for 12h, 24h and 48h in juvenescent mice; The hemi-ovaries are cultured for 3h in vitro before heterotrophic autotransplantation, then to observe the differences of blood vessel density in different transplanting sites,and bloodstream
