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1、1anthocyanidin configuration and biosynthetic pathways hypothesisTIAN Xiao-Yan(Shenyang Agriculture University Food Institute, Liaoning Province Shenyang,110161, in China)MAbstract This study was carried out on the mass spectrometry analysis of Nanguo pear fruit peel anthocyanidin, after detailed ca
2、lculation of MS and +MS2, hypothesized that the anthocyanidin configuration in nature exist is in the form of lantern body or globule body, and its compositions either only anthocyanidin unit, or associating with glucoses. The lantern body or globule body is connected between the cell membrane or cy
3、toplasm membrane or garna membrane and photosynthesis system. These anthocyanidins have specific molecular weight, shape and specific spatial configuration. Six kinds of anthocyanidins, as usually proposed, called pelargonidin, cyanidin, delphinidin, peonidin, petunidin, malvidin, perhaps only very
4、little exist in nature at monomers, almost all anthocyanidins exist in nature at lantern body or globule body. Anthocyanidin is a reticular macromolecules consisting of phenyl benzopyrane connected through oxygen bridge similar as a lantern body or globule body. A layer 4B rings link a layer 4phenyl
5、 benzopyranes through 4 C of B ring and 5 C of A ring of phenyl benzopyrane by oxygen bridge tied together longitudinally, while 2C, 7C of A ring of phenyl benzopyrane by oxygen bridge hand in hand tied together horizontally, repeating this combination units until its lenghth meeting the needs. Some
6、 4phenyl benzopyranes of lantern body anthocyanidin link with Mg2+, its function resembles some chlorophyll a, can absorb and convert light energy. If 3C or 5C of B ring has no oxygen bridge linking each other, after acidulated, get pelargonidin; if 3 and 5 of B ring only link one or two oxygen brid
7、ges, get cyanidin and pelargonidin; if 3 and 5 of 4B rings has three or four oxygen bridges, get delphinidin and cyanidin; if position 3 of B ring only links one methoxy, get peonidin; if 3 of B ring links one methoxy, while position 5 of B ring links one oxygen bridge, get petunidin; if 3 and 5 of
8、B ring link two methoxy, get malvidin. In general, pelargonidin, cyanidin, delphinidin exist in inner of anthocyanidin; peonidin, petunidin, malvidin exist in top of anthocyanidin for connecting with the cell membrane or the photosynthesis system, so the content of peonidin, petunidin, malvidin is r
9、elatively rare. Color differences of flowers and fruits, lie on the contents of anthocyanidin and abundance of oxygen bridges. While the contents of anthocyanidin and abundance of oxygen bridges are higher, spectral blue shift. The major function of anthocyanidin is absorbing ultraviolet rays, trans
10、forming high light energy to low energy for the photosynthesis, so prevent the ultraviolet rays injure on flowers and fruits. The study considers that flowers and fruits possess the features of photosynthesis. Anthocyanidin macromolecules just like Sky-net covering on the breakage of chloroplast mem
11、branes or granum membranes damaged by Uv, Uv high-energy photons activate high-energy electrons, when high-energy electrons pass through p- conjugation rings of anthocyanidin macromolecules, rotating with high speed in p- conjugation rings, producing heat and released out, at the same time, high-ene
12、rgy electrons turned into low-energy electrons transporting to the carbon reaction system, so as to ensuring the carbon reaction system can effectively carry out photosynthesis. This hypothesis considers anthocyan biosynthesis main substrates is glucose and acetyl coA. introduction:2A large number o
13、f experiments have been carried out in orchard and laboratory aiming at raising the contents of anthocyanidin of Nanguo pear peel. Nanguo pear is special local product of Anahan city and Haicheng city in Liaoning Province of China, Nanguo pear is a variant of Pyrus ussuriensis Maxim, its aroma is wo
14、nderful and rich tasting, can be the king of pear. But, it has a fatal defect, that is fruit depigmentation when meeting autumn rains in maturation period, accompanying with fruit quality sharp declining, and again colouring is hard. For solveing the difficult problem, carried out on the mass spectr
15、ometry analysis of Nanguo pear fruit peel anthocyanidin, accidently, discovered the reasonable framework of anthocyanidin in nature. It is a complete net macromolecule or globule, not fragment. 1 Materials and Methods1.1 materialsTen Nanguo pear fruits were picked up from 10-12 years Nanguo pear, in
16、 the south direction, middle part, and equal height, 3 repeats, On august 25, 2010, in Song village of Jiewen town of Haicheng city in Liaoning province.Chemical reagents: Hydrochloric acid, 95% ethanol, muriate, anhydrous sodium acetate is analytical reagent of the shenyang chemical reagent factory production; D101 macroporous resin coming from the Chinese medicine group chemical reagent co., LTD; ethyl alcohol, methane acid is chromatographicall pur
