《紫外分光光度法测定马齿苋总黄酮的含量》由会员分享，可在线阅读，更多相关《紫外分光光度法测定马齿苋总黄酮的含量（3页珍藏版）》请在金锄头文库上搜索。
1、D2010M623null Researchof Integrated Traditional Chineseand WesternMedicine2010 June Vol.2 No.3cI|:1674null4616(2010) 03null0126null03nullYT, Corresponding author, Enullmail: felu tjhnull tjmunull edunull cn,s;E9c雷红伟1, 2 null null 陆付耳1null null null 徐丽君1 null null 董null 慧1 null null 邹null 欣1 null nul
2、l 黄召谊11 华中科技大学附属同济医院中西结合研究所,武汉null 4300302 湖北中医药大学药学院,武汉null 430065null nullK1nullnull 从马齿苋(portulacaoleracea L.)中分离a制备总黄酮,用紫外分光光度法测定其含量bZEnull 用含3%( g/ ml)氢氧化钠和60%( ml/ml)乙醇碱性醇液煎煮提取总黄酮,聚酰胺柱树脂柱层析分离纯化b以芦丁为对照品,以亚硝酸钠null硝酸铝null氢氧化钠为显色剂,用分光光度计在510 nm 可见光处测定黄酮含量bTnull 芦丁标准品溶液浓度在8 48 mg/L 范围内与吸光度有良好线性关系,回
3、归方程为Y= 0null005X- 0null001, r2= 0null 999 8,精密度实验RSD为0null69%,稳定性实验RSD为2null04%,重复性实验RSD为2null1%,平均回收率为98null 55%,RSD为0null 58%,马齿苋全草中总黄酮的含量为7null 67%bnull 本法测定马齿苋总黄酮的结果准确,可靠,能用于测定马齿苋全草中总黄酮的含量bnull null1oMnull 马齿苋; null 总黄酮; null 紫外分光光度计Determinationof theContentof TotalFlavoniodsfromPortulaca Olera
4、cea L.byUVSpectrophotometryLEI Hongwei1, 2, LU Fuer1null , XU Lijun1, et al1Department of Integrated Traditional Chinese and Western Medicine,Tongji Hospital,Tongji Medical College,Huazhong University of Science and Technology,Wuhan430030,China2Pharmacy College of Hubei University of Traditional Chi
5、nese Medicine,Wuhan430065,Chinanull null Abstractnull Objectivenull To separate and purify total flavonoids from portulaca oleracea L . and determinate itscontent by UV spectrophotometrynullMethodsnull The total flavonoids from portulaca oleracea L . were extracted by60% ethanol and 3% sodium hydrox
6、idenullThen separated and purified by polyamide resin chromatographynullBased onreference substance of rutin, with NaNO2nullAl( NO3 ) 3nullNaOH as chromogenic agent, the determination was pernullformed by UV spectrophotometry at the wave length of 510 nmnullResultsnull The absorbency and concentrati
7、on of runulltin have good linear correlation in the range of 8null48 mg/LnullT he regression equation was Y= 0null005Xnull0null001(r2 =0null999 8)nullAccuracy experiment RSD= 0null69%, stability test RSD= 2null04%, repeatability test RSD= 2null1%, the avnullerage recovery rate was 98null55%, RSD= 0n
8、ull58%nullThe content of total flavonoids in portulaca oleracea L . was7null67%nullConclusionnull The method is simple and fast with good stability, accuracy and repeatabilitynullIt can be appliedto determinate the content of total flavonoids in portulaca oleracea Lnull.null null Keywordsnull portul
9、aca oleracea L.; total flavonoids; UV spectrophotometrynull nulli73,(portulacaoleracea L.)c73B 1bae,baahTbha%a/a02a 2bC0 2null4VF)aaFaaFaea#gT,t0T(1b- 5null7?Canull126nullD2010M623null Researchof Integrated Traditional Chinese and Western Medicine2010 June Vol.2 No.3T,1b3,v7?Nb9c,aT,sUh01ilb#Ls;E9c,
10、OFT4$b1nullZE1null1null0k4(30null60,|: 20071018)1S0vk4K,Rn(|: 20070706)1S30_,b)(|:20070916)abh(|: 20071128)a_)(|: 20080227)(1?0Cu?vk4,Y?(|: 20071015)1?0Ck4K,(6D04)D0v30ik( portulacaoleraceaL. )b1null2nullNUVnull1900,s;9(H ITACHI,), Rnull200?N( BUCHTI,V) ,BT25null1/100?(/K,)b1null3nullZE1null3null 1
11、nullr!nullD 8,|521 kg,Fc3%(g/ml)_)60%( ml/ml)Y?a?A6 L,m4|2 h,l0A,:0?Fa?A6 L,TQ,GQF1 h0null5hbi3Q0A,lY?,iAb|iAYV( 10 cm null 40 cm),?!rAbKY?!,!AH,lNW!Ab|!A?Nic306 kg/ LiA,rb1null3null 2 null_onullD9R#hA200 800 nmW,510 nm)KvlbyN4510 nmo9cob1null3null 3 null9cLA!nullM|1 mlriA100 ml,F100 ml,c3060 g/ LA,|1 ml20 mlF20 ml,c303 g/LA,ii!b1null3null4 nullRSwLL1null|0null2mg/mlSA0null4a0null8a1null 2a1null6a2null02null4 ml,sY10 ml,F2null4 ml,F5%b)0null4 ml,(,6 min,
