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1、 5 1 J_ Vo l. 5 No. 1 2014M 1 Journal of Food Safety and Quality Jan. , 2014 基金项目: _fS/ (SZ2011001); SES/905 (2012BA08B01-5) Fund: Supported by Shenzhen CIQ Science & Technology Program (SZ2011001) and The national science and Technology Support Program (2012BA08B01-5) *通讯作者: uY , V , = , 1Z_J3#u0=_
2、b E-mail: *Corresponding author: ZHAO Fang, Engineer, Shenzhen Entry-Exit Inspection and Quarantine Bureau, No.1011, Fuqiang Road, Futian Dis-trict, Shenzhen 518045, China. E-mail: zf.f AlphaLISA/J_Z 洪小柳 , 吕敬章 , 张 恒 , 范 放 , 刘慧玲 , 赵 芳*(_f , 518045) 摘 要: ,(MHWsO;fE (AlphaLISA)B,;?;(M_/ , .df (ELISA)
3、(Mab!aa+s%h?31 , a%hb E3 B10S , .d/Y , if/bAlphaLISA E3_?MYW E3 y0 , l?hbZEW07?|4S:r銪0F8 , EWT , _2 5 ng/L, _S 0.01100 g/LbLICLIA EK1Yy4ad1 , L4 Enrofloxacin-OVA-?; 1: 500aF6vXH 1: 2500a3FtF8 1: 200 id1 , Q130 min, ZE2 2 ng/L, _S0.007100 g/L, |f_ZEfsE (ELISA)ZEM11 , TVZEM1z , LICLIA E2K , V_b o (Chl
4、oramphenicol)BF3brTz#N , X$REZaEa#.%hb Zhang 20nQ AlphaLISA Ed/ao (CAP)_H , yCAP Tls0 , Ff , #E , v , CAP-BSA CAP-OVA bZE20.0086 ng/mL, ELISAZE2 0.05 ng/mL, _S 0.009620 ng/mL,Vr 3-5 ) , T 30 min= , V 384a1536a3456 dY_b 4 展 望 M , J1jA , J31v , yyJu03=2y_ZEX$b- , Ju03=_ZEEaAEabs8E (Charm II)fE (ELISA)
5、21bEAE_2a+sRiNL , ZE , TP , k4hvK , ay_/ ; bs8E (Charm II)EVrg/kg(ppb)_2 , _SK , 7O_/ , wsK ; fE (ELISA)TeLa_Y7$W , -KrRP_/ , J_F)?Z , .d ELISA 2X?y_1pb AlphaLISA /_5C_/ , $! ELISA, ya(M(! )a2azTeL+22,23, dLy1b- , Y3Ss1_N1pM , AlphaLISA/|h)a 3F3=y_?ZZ_b 参考文献 1 Ullman EF, Kirakossian H, Singh S, et a
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7、s PG, Leong K, Day TA, et al. Development of homoge-neous 384-well high-throughput screening assays for a1-40 and a1-42 using alphascreen technology J. J Biomol Screen, 2008, 13(2): 101-111. 4 , , a , . AlphaLISA /?Z# J. Ds03 , 2011, 8(3): 261264. Yang Y, Song J, Cui Y, et al. Development and Applic
8、ation of AlphaLISA Technology J. J Med Mol Biol, 2011, 8(3): 261264. 5 Rouleau N, Silva LR, Ryabov I, et al. Development of a Non-Radioactive, No-Wash Biochemical Assay for High-Throughput Screening of Small Molecule Modulators of 202 J_ 5 DNA Methyltransferases C. Proc SBS Conf, 2011. 6 Bouchecarei
9、lh M, Caruso ME, Roby P, et al. Alpha-Screen-Based Characterization of the Bifunctional Ki-nase/RNase IRE1A Novel and Atypical Drug Target J. J Bio-mol Screen, 2010, 15(4): 406-417. 7 Mechaly A, Cohen N, Weiss S, et al. A novel homogeneous im-munoassay for anthrax detection based on the AlphaLISA me
10、-thod: detection of B. anthracis spores and protective antigen (PA) in complex samples J. Anal Bioanal Chem, 2013: 1-8. 8 Simard JR, Plant M, Emkey R, et al. Development and imple-mentation of a high-throughput AlphaLISA assay for identifying inhibitors of EZH2 methyltransferase J. Assay Drug Dev Tech-nol, 2013, 11(3): 152-162. 9 Nutu M, Zetterberg H, Londos E, et al. Evaluation of the Cere-brospinal Fluid Amyloid-1-42
