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1、TheVeterinaryRecord,November23,1996 ShortCommunications Felinecoronavirusinthe intestinalcontentsofcatswithfeline infectiousperitonitis D.D.Addie,S.Toth,A. A.P.M.Herrewegh,0.Jarrett VeterinaryRecord(1996)139,522-523 THEquestionofwhetherornotcatswithclinicalfelineinfectious peritonitis(FIP)excretethe
2、causativeagent,felinecoronavirus (FCoV),isimportanttothepractisingveterinarysurgeonfortwo reasons.First,theyhavetoknowwhetheracatwithFIPintheir surgeryposesahazardtoothercats.Secondly,theymayhaveto adviseownersofacatwithFIPwhetheritissafetoobtainanother catwhiletheaffectedcatisstillalive(bearinginmi
3、ndthatcats withdryornon-effusiveFIPcanliveformanyweeksormonths). Inexperimentalinfections,catswithFTPhavebeenshownnotto shedFCoVbythetimetheyareclinicallyill(Stoddartandothers 1988).However,inthiscommunication,theauthorsshowthat FCoVcanbedetectedinthefaecesandintestinalcontentsofcats whicharenatural
4、lyinfectedandsufferingfromFIP. Themostintractableobstacletothecompleteunderstandingof theepidemiologyofFlPhasbeenthenearimpossibilityofdetect- ingFCoVininfectedcats.Mostattemptstoisolatethevirusincell culturefromcasesofFIP,orfromcatssuspectedofcarryingthe virus,havefailedandonlyabout20isolateshavebe
5、enmade worldwidesincetheviruswasidentifiedover30yearsago.The recentdevelopmentofthereversetranscriptase-polymerasechain reaction(RT-PCR)todetectFCoVRNA,presumablyrepresenting wholevirus,infaecesandbodyfluidsofcats(Herreweghandoth- ers1995)maycircumventsomeoftheseproblemsandprovidean opportunitytoans
6、wersomeoftheoutstandingquestionsaboutthe transmissionofFCoV.Inthiscommunicationtheresultsofastudy todeterminewhethercatswithnaturallyoccurringFIPexcrete FCoV,byexaminingtheirgutcontentsforthepresenceofFCoV RNAbyRT-PCR,arepresented. Histopathologicalsamples,blood,asciticfluidandfaecesor intestinalcon
7、tentsfromcatssuspectedofhavingFIPwere obtainedonthedayofdeath.FIPwasconfirmedbyhistopathology aspreviouslydescribed(Addieandothers1995).Samplesofthe contentsofstomach,smallorlargeintestine,orfaeceswereavail- ablefrom23catswitheffusiveFIPandfivecatswithnon-effusive FIP.Thesampleswerestoredat-80Cuntil
8、tested.FCoVwas detectedbyRT-PCR(Herreweghandothers1995).Faecesfroma specificpathogenfree(SPF)catservedasanegative,FCoV-unin- fectedcontrol.Inaddition,materialfromthreeseronegativecats withlymphosarcoma,intestinaladenocarcinomaandfelineparvo- virusinfectionandaseropositivecat(anti-FCoVtitreof320) D.D
9、.Addie,S.Toth,0.Jarrett,DepartmentofVeterinaryPathology, UniversityofGlasgowVeterinarySchool,BearsdenRoad,Glasgow G61tQH A.A.P.M.Herrewegh,DepartmentofVirology,FacultyofVeterinary Medicine,UtrechtUniversity,Utrecht,TheNetherlands TABLE1:ResultsofRT-PCRtestingforFCoVinthegutcontentsofcats withhistopa
10、thologicallyconfirmedFIP Cat 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24 25 26 27 28 29 30 31 32 33 Sample intestinalcontents* stomachcontents Sicontents Sicontents LIcontents LIcontents Sicontents faeces Sicontents intestinalcontents* faeces rectalswab faeces Sicontents faeces LIco
11、ntents faeces Sicontents Sicontents faeces faeces faeces Sicontents 1monthbeforedeath 8monthsbeforedeath Sicontents stomachcontents duodenalcontents jejunalcontents ilealcontents caecalcontents LIcontents LIcontents intestinalcontents* Sicontents stomachcontents duodenalcontents ilealcontents jejuna
12、lcontents caecalcontents coloncontents rectalcontents intestinalcontents* Sicontents faeces faeces faeces IFAtitre 20 40 80 160 160 160 320 320 320 640 640 640 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 1280 Non-FIPs 0 320 0 0 TypeofFIP effusive effusive effusive effu
13、sive effusive effusive non-effusive effusive non-effusive non-effusive effusive effusive effusive effusive effusive non-effusive effusive non-effusive effusive effusive effusive effusive effusive effusive effusive effusive effusive parvovirus lymphosarcoma lymphocyticcholangitis SPF adenocarcinoma P
14、CR SiSmallintestine,LiLargeintestine,*Exactareaofcollectionunknown whichdiedofcholangitisandhadnohistopathologicalevidence ofFIPwastested.Anti-Fcovantibodiesinplasmaorasciticfluid weremeasuredbyimmunofluorescence(AddieandJarrett1992). AsshowninTable1,22ofthe28catswithnaturallyoccurring FIPwerepositi
15、veforFCoVRNAbyRT-PCR.Ofthesixcatswhich werenegative,fourofthesamplestestedwereofsmallintestinal contentscollectedatpostmortemexamination,sothattheques- tionaroseofwhetherFCoVwaseithernotpresentinsmallintesti- nalsamplesorpresentbutintoosmallquantitiestodetect(and possiblybeingconcentratedfurtherdown
16、inthebowel).Samples fromallpartsofthegutwereavailableforonlytwocats,cats24 and28,andviruswasdetectedonlyinthecaecumofcat24andin 522 on June 22, 2015 - Published by from TheVeterinaryRecord,November23,1996 allareasofthegastrointestinaltractofcat28.Overall,atdeath, fiveof12smallintestinalcontentswerenegative,comparedwith onlytwoof15largeintestinalcontents,faecalsamplesorrectal swabs.Theseproportionsarenotsignificantlydifferent(P=0.18, Fishersexacttest). Allofthesamplesfromcatswithnon-effusiveF
