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1、 .限制性酶的识别序列Enzyme Name Sequence AatI AGGCCT AatII GACGTC AccI GTMKAC AccII CGCG AccIII TCCGGA AcyI GRCGYC AflI GGWCC AflII CTTAAG AflIII ACRYGT AhaI CCSGG AhaII GRCGYC AhaIII TTTAAA AluI AGCT AlwI GGATCNNNN AlwI NNNNNGATCC AlwNI CAGNNNCTG AocI CCTNAGG AocII GDGCHC AosI TGCGCA AosII GRCGYC ApaI GGGCC
2、C ApaLI GTGCAC ApyI CCWGG AquI CYCGRG AseI ATTAAT AspI GAANNNNTTC AspI GGTACC AspI GACNNNGTC AsuI GGNCC AsuII TTCGAA AvaI CYCGRG AvaII GGWCC AvaIII ATGCAT AvrI CYCGRG AvrII CCTAGG AxyI CCTNAGG BalI TGGCCA BamHI GGATCC BanI GGYRCC BanII GRGCYC BanIII ATCGAT BbeI GGCGCC BbiII/AcyI GRCGYC BbvI GCAGCNNN
3、NNNNN BbvI NNNNNNNNNNNNGCTGC BbvII GAAGACNN BbvII NNNNNNGTCTTC BcefI ACGGCNNNNNNNNNNNN BcefI NNNNNNNNNNNNNGCCGT BclI TGATCA BcnI CCSGG BglI GCCNNNNNGGC BglII AGATCT BinI NNNNNGATCC BinI GGATCNNNN BsePI GCGCGC BsmAI GTCTC BsmAI GAGAC BsmI GAATGCN BsmI GCATTC BspI GDGCHC BspHI TCATGA BspMI ACCTGCNNNN
4、BspMI NNNNNNNNGCAGGT BspMII TCCGGA BsrI ACTGGN BsrI CCAGT BssHII GCGCGC BstBI TTCGAA BstEII GGTNACC BstI GGATCC BstNI CCWGG BstPI GGTNACC BstUI CGCG BstXI CCANNNNNNTGG BstYI RGATCY BsuI CCTNAGG CcrI CTCGAG CfoI GCGC CfrI RCCGGY CfrI GGNCC CfrI YGGCCR ClaI ATCGAT CviJI RGCY CvnI CCTNAGG DdeI CTNAG Dp
5、nI GATC DraI TTTAAA DraII RGGNCCY DraIII CACNNNGTG DsaI CCRYGG EaeI YGGCCR EagI CGGCCG EarI CTCTTC EarI GAAGAG EclXI CGGCCG EcoI TACGTA EcoI GGTCTCN EcoI NNNNNGAGACC EcoI GGWCC EcoIII AGCGCT EcoI CGGCCG EcoI CTGAAG EcoI CTTCAG EcoI CCTNAGG EcoNI CCTNNNNNAGG EcoOI RGGNCCY EcoRI GAATTC EcoRII CCWGG EcoRV GATATC EcoTI CCWWGG EcoTI ATGCAT EcoTI GRGCYC EheI GGCGCC EspI GCTNAGC FinI GTCCC FinI GGGAC FnuHI GCNGC FokI GGATGNNNNNNNNN FokI NNNNNNNNNNNNNCATCC FspI TGCGCA GdiII NNNNNYGGCCG GdiII CGGCCRN GsuI CTCCAG GsuI CTGGAG HaeI WGGCCW HaeII RGCGCY HaeIII GGCC
