16S-rDNA引物资料

《16S-rDNA引物资料》由会员分享，可在线阅读，更多相关《16S-rDNA引物资料（2页珍藏版）》请在金锄头文库上搜索。

1、<p>&lt;p&gt;&amp;lt;p&amp;gt;&amp;amp;lt;p&amp;amp;gt;&amp;amp;amp;lt;p&amp;amp;amp;gt;16S-rDNA引物一般细菌鉴定选择通用引物，最常用的通用引物为27F/1492R。27F：5&amp;amp;amp;amp;#39;-AGAGTTTGATCCTGGCTCAG-3&amp;amp;amp;amp;#39;1492R：5&amp;amp;amp;amp;#39;-GGTTACCTTGTTACGACTT-3&

2、amp;amp;amp;amp;amp;#39;Lane, D. J. (1991). 16S/23S rRNA sequencing. Nucleic acid techniques in bacterial systematics. E. Stackebrandt and M. Goodfellow, eds. New York, NY, John Wiley and Sons: 115-175.&amp;amp;amp;amp;#183; 27f 5&amp;amp;amp;amp;#39;AGAGTTTGATCMTGGCTCAG&amp;amp;amp;amp;

3、#183; 342r 5&amp;amp;amp;amp;#39;CTGCTGCSYCCCGTAG&amp;amp;amp;amp;#183; 357f 5&amp;amp;amp;amp;#39;CTCCTACGGGAGGCAGCAG&amp;amp;amp;amp;#183; 519r 5&amp;amp;amp;amp;#39;GWATTACCGCGGCKGCTG&amp;amp;amp;amp;#183; 530f 5&amp;amp;amp;amp;#39;GTGCCAGCMGCCGCGG&amp;amp;amp;amp

4、;#183; 1100r 5&amp;amp;amp;amp;#39;GGGTTGCGCTCGTTG&amp;amp;amp;amp;#183; 1114f 5&amp;amp;amp;amp;#39;GCAACGAGCGCAACCC&amp;amp;amp;amp;#183; 1392r 5&amp;amp;amp;amp;#39;ACGGGCGGTGTGTRC&amp;amp;amp;amp;#183; 1406f 5&amp;amp;amp;amp;#39;TGYACACACCTCCCGT&amp;amp;amp;amp;#

5、183; 1492r 5&amp;amp;amp;amp;#39;TACGGYTACCTTGTTACGACTT&amp;amp;amp;amp;#183; 1525r 5&amp;amp;amp;amp;#39;AAGGAGGTGWTCCARCCUniversal primers from Leser02&amp;amp;amp;amp;#183; Edited from Lane91, naming from Alm96,&amp;amp;amp;amp;#160;PMID 8837410&amp;amp;amp;amp;#183; S-*-U

6、niv-1492-a-A-19 GGTTACCTTGTTACGACTT-3&amp;amp;amp;amp;#183; S-D-Bact-0008-a-S-20 AGAGTTTGATCMTGGCTCAG-3&amp;amp;amp;amp;#183; S-D-Bact-0907-a-S-20 AAACTCAAAGGAATTGACGG-3&amp;amp;amp;amp;#183; S-*-Univ-0519-a-A-18 GTATTACCGCGGCTGCTG-3&amp;amp;amp;amp;#183; S-D-Bact-0786-a-A-20 GACTACC

7、AGGGTATCTAATC-3&amp;amp;amp;amp;#183; S-D-Bact-1054-a-A-20 ACGAGCTGACGACAGCCATG-3Mycoplasma universal primersJohansson K-E, Characterization of mycoplasmas by PCR and sequence analysis with universal 16S rDNA primers, Chapter 18, Mycoplasma Protocols, 1998.&amp;amp;amp;amp;#183; U1F 12-27 tt

8、g atc ctg gct cag gay daa cg&amp;amp;amp;amp;#183; U2F 327-348 cca rac tcc tac ggr agg c&amp;amp;amp;amp;#183; U3R 512-527 att acc gcg gck gct g&amp;amp;amp;amp;#183; U4F 792-810 gta gtc cac gcc gta aac g&amp;amp;amp;amp;#183; U5R 902-924 ctt gtg cgg gyy ccc gtc aa&amp;amp;amp;am

9、p;#183; U6F 1154-1172 gag gaa ggy grg gay gay g&amp;amp;amp;amp;#183; U7R 1359-1374 aca agr ccc gag aac g&amp;amp;amp;amp;#183; U8R 1502-1524 gaa agg agg trw tcc ayc csc ac&amp;amp;amp;lt;/p&amp;amp;amp;gt;&amp;amp;lt;/p&amp;amp;gt;&amp;lt;/p&amp;gt;&lt;/p&gt;</p>