《基因工程作业(引物设计)讲解》由会员分享，可在线阅读，更多相关《基因工程作业(引物设计)讲解（12页珍藏版）》请在金锄头文库上搜索。
1、口腔鳞癌组织中肿瘤转移相关基因1（MTA1）一、选择原因及应用口腔鳞癌组织中肿瘤转移相关基因1（MTA1）在蛋白和mRNA的表达水平，揭示其与口腔鳞癌（OSCC）发生、发展的关系。方法采用免疫组织化学法和原位杂交技术检测46例OSCC标本、15例口腔黏膜白斑与20例正常口腔黏膜标本中MTAl基因的表达水平，并分析其与OSCC临床病理学参数的关系。结果MTA1蛋白和MTA1mRNA在OSCC组织中的表达水平显著高于口腔黏膜白斑和正常口腔黏膜（P005），口腔黏膜白斑中MTA1蛋白和MTA1mRNA表达水平显著高于口腔正常黏膜（P001），MTA1蛋白和MTA1mRNA表达与肿瘤浸润深度和淋巴结转
2、移密切相关（P005）。结论MTA1基因在蛋白和mRNA的表达水平在OSCC发生、发展及浸润转移过程中起一定促进作用，有望成为判断OSCC预后及选择治疗方案的一个新肿瘤标志物。2、 2查阅NCBI得到MTA1相关信息并获得目的基因PREDICTED: Gorilla gorilla gorilla metastasis associated 1 (MTA1), mRNANCBI Reference Sequence: XM_004055801.1FASTA Graphics Comment Features SequenceLOCUS XM_004055801 2872 bp mRNA lin
3、ear PRI 03-DEC-2012DEFINITION PREDICTED: Gorilla gorilla gorilla metastasis associated 1 (MTA1), mRNA.ACCESSION XM_004055801VERSION XM_004055801.1 GI:426378238KEYWORDS .SOURCE Gorilla gorilla gorilla (western lowland gorilla) ORGANISM Gorilla gorilla gorilla Eukaryota; Metazoa; Chordata; Craniata; V
4、ertebrata; Euteleostomi; Mammalia; Eutheria; Euarchontoglires; Primates; Haplorrhini; Catarrhini; Hominidae; Gorilla.COMMENT MODEL REFSEQ: This record is predicted by automated computational analysis. This record is derived from a genomic sequence (NW_004005914) annotated using gene prediction metho
5、d: GNOMON, supported by mRNA and EST evidence. Also see: Documentation of NCBIs Annotation Process #Genome-Annotation-Data-START# Annotation Provider : NCBI Annotation Status : Full annotation Annotation Version : Gorilla gorilla Annotation Release 100 Annotation Pipeline : NCBI eukaryotic genome an
6、notation pipeline Annotation Method : Best-placed RefSeq; Gnomon Features Annotated : Gene; mRNA; CDS; ncRNA #Genome-Annotation-Data-END#FEATURES Location/Qualifiers source 1.2872 /organism=Gorilla gorilla gorilla /mol_type=mRNA /sub_species=gorilla /db_xref=taxon:9595 /chromosome=14 gene 1.2872 /ge
7、ne=MTA1 /note=Derived by automated computational analysis using gene prediction method: GNOMON. Supporting evidence根据 includes similarity to: 4 mRNAs, 303 ESTs, 7 Proteins /db_xref=GeneID:101134898 misc_feature 104.106 /gene=MTA1 /note=upstream in-frame stop codon CDS 212.2359 /gene=MTA1 /codon_star
8、t=1 /product=metastasis-associated protein MTA1 /protein_id=XP_004055849.1 /db_xref=GI:426378239 /db_xref=GeneID:101134898 /translation=MAANMYRVGDYVYFENSSSNPYLIRRIEELNKTANGNVEAKVVC FYRRRDISSTLIALADKHATLSVCYKAGPGADNGEEGEIEEEMENPEMVDLPEKLKHQ LRHRELFLSRQLESLPATHIRGKCSVTLLNETESLKSYLEREDFFFYSLVYDPQQKTL L
9、ADKGEIRVGNRYQADITDLLKEGEEDGRDQSKLETKVWEAHNPLTDKQIDQFLVVAR SVGTFARALDCSSSVRQPSLHMSAAAASRDITLFHAMDTLHKNIYDISKAISALVPQG GPVLCRDEMEEWSASEANLFEEALEKYGKDFTDIQQDFLPWKSLTSIIEYYYMWKTTD RYVQQKRLKAAEAESKLKQVYIPNYNKPNPNQISVNNVKAGVVNGTGAPGQSPGAGRA CESCYTTQSYQWYSWGPPNMQCRLCASCWTYWKKYGGLKMPTRLDGERPGPNRSNMSP HGLPAR
10、SSGSPKFAMKTRQAFYLHTTKLTRIARRLCREILRPWHAARHPYLPINSAAI KAECTARLPEASQSPLVLKQAVRKPLEAVLRYLETHPRPPKPDPVKSVSSVLSSLTPA KVAPVINNGSPTILGKRSYEQHNGVDGNMKKRLLMPSRGLANHGQTRHMGPSRNLLLN GKSYPTKVRLIRGGSLPPVKRRRMNWIDAPDDVFYMATEETRKIRKLLSSSETKRAAR RPYKPIALRQSQALPLRPPPPAPVNDEPIVIEDORIGIN 1 tcctcctctt cctctcccgc ccgcgc
11、cgcg gccctcccgt ccctgcgcgg cctcggcggc 61 ctcggcggcg gcggcggcgg cggcggcagc agcgcggccc ctttaaacgc ctgcggcgcc起始密码子 121 ccccgccccc gccatcgcgc ctccattttc ccggccgccc gcgccgagcg ccgcgcccgc 181 cccgggcccc tccgccgccg ccggcccgga catggccgcc aacatgtaca gggtcggaga 241 ctacgtctac tttgagaact cctccagcaa cccatacctg
12、atccggagga tcgaggagct 301 caacaagacg gccaatggga acgtggaggc caaagtggtg tgcttctacc ggaggcggga 361 catctccagc accctcatcg ccctggccga caagcacgca accctgtcag tctgctataa 421 ggccggaccg ggggcggaca acggcgagga aggggaaata gaagaggaaa tggagaatcc 481 ggaaatggtg gacctgcccg agaaactaaa gcaccagctg cggcatcggg agctgttcct 541 ctcccggcag ctggagtctc tgcccgccac gcacatcagg ggcaagtgca gcgtcaccct 601 gctcaacgag accgagtcgc tcaagtccta cctggagcgg gaggatttct tcttctattc 661 tctagtctac gac
