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1、 Parasitic Pathogen Examination寄生虫病原检查寄生虫病原检查Etioligic DiagnosisStool, blood, marrow, urine, sputum, humores(体液体液), secretion and tissue. Purpose of examination Examining pathogen, identifying the species of parasite and the degree of infection. Specimen: Blood and marrow examination Examining plasm
2、odium the collection of blood time： blood smear thin smear： thick smear： stainWright stainGiemsa stain Practice 1 Practicing thin smear, stain and observing Plasmodium berghei.薄血膜制片薄血膜制片 厚血制片厚血制片取血与涂片取血与涂片固定与染色固定与染色 固定固定 薄血膜晾干后薄血膜晾干后，甲醇固定甲醇固定 染色染色 将姬氏染液滴于已固定的薄血将姬氏染液滴于已固定的薄血膜上，染色半小时（室温），自膜上，染色半小时（室温）
3、，自来水冲洗。血片晾干后镜检。来水冲洗。血片晾干后镜检。 Blood and marrow examination Examining microfilaria from bloodTime for blood collectingMethods: Examining L.donovani from marrow Aspiration (穿刺检查穿刺检查):Stool Examination Collect the stool in a dry, clean, leakproof container. Make sure no urine, water, soil or other mater
4、ial gets in the container. Label the container with the patients name , date and time of collection. Significance: Caution:Stool Examination Before the patient taking medicine the stool specimen should be collected. Fresh stool should be examined, or preserved immediately. Macrography( (肉眼观察）肉眼观察） W
5、et mount examination ( (Direct smear ) ) Concentration methods Larva hatching methods How to find parasites in the feces?Stool Examination Wet mount method: Direct smear with saline Iodine staining method（碘染色法）碘染色法）Stool Examination Concentration method: modified Kato-Katzs method sedimentation meth
6、ods flotation methods Flotation method:Stool Examination Zinc sulfate centrifugal flotation Saturated brine flotation Sucrose sugar flotation gravitational sedimentation method自然沉淀法自然沉淀法 centrifugal sedimentation method离心沉淀法离心沉淀法Stool Examination Sedimentation method: larva hatching method: miracidi
7、um hatching method culture method for hookworm larva How to confirm the density of parasite or worm burden? Modified Kato-Katzs method Stolls eggs counting Practice 2Self-examining stool yourself! Direct smear with saline Saturated brine flotation Examining parasite from perianal skin Parasite eggs
8、and adults of pinworm eggs of Taenia Methods cellophane tape method（透明胶纸法）透明胶纸法） cotton swab method （棉拭子法）棉拭子法） Sputum, Urine, Vaginal secretion, Duodenal fluid, Humores, CSF-Biopsy and animal inoculationSummary Principles of different methods of parasitic diagnosis. ( you should master them.) The processes of experiments. Preparing for the next class Contents: Principles Experiments Medical terms
