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1、CAPTER 5 COMPLEMENT（补体）DEPARTMENT OF IMMUNOLOGYSICHUAN UNIVERSITYE-MAIL:DEFINITION Bacteria + Antibody + normal serumSoluble（细菌溶解）Bacteria +antibody + 55 prepared serum（55）Insoluble（细菌不溶解）What you learn from this experimentWhat is complement A group of heat-labile normal proteins which distribute in
2、 serum,humour and some cell membrane.They closely relate to the immune function and have enzymatic activation after actived（分布于血清，体液和一些细胞膜表面的一组热不稳定正常蛋白。与免疫功能紧密相关，活化后具有酶活性）What is the property of the complement（补体有和特点）1. heat-labile （热不稳定）2. normal proteins ,is independent of antigen stimulation（正常蛋白
3、，与抗原刺激无关）3.not a singly,there are about 30 kind of protein consist of a group,called complement system（不是一个单一成分，大约30种蛋白组成补体系统）4.have enzymatic activation（具有酶活性）5.play an important rloe in immune function（在免疫功能中发挥重要作用）The consist of complement system（补体系统的组成）1.the immanent compose（固有成分） C1C9,D,B,P fa
4、ctor,MBL,serine protease（苏氨酸蛋白酶）2.the regulative protein（调节蛋白）3.the complement receptors（补体受体）THE PATHWAY OF COMPLEMENT ACTIVATED（补体活化途径）THREEDIFFERENTPATHWAYS（三条途径）1.Classicalpathway（经典途径）2.Alternativepathway（替代途径）3.Lectinpathway（凝集素途径，MBL途径）CLASSICALPATHWAYcommonly initiated by the formation of so
5、luble antigen-antibody complexes or by the binding of antibody to antigen on a suitable target （有可溶性抗原-抗体复合物或者抗体结合到相应靶细胞上启动）The condition of Complement activation via the classical pathway（经典途径活化补体的条件） 1. The formation of antigen-antibody complexes （抗原-抗体复合物的形成）2.only C1 bind with CH3 of IgM or CH2
6、of IgG1,IgG2,IgG3（C1与IgM的CH3或IgG1,IgG2,IgG3的CH2功能区结合）3.one C1 must bind with at least two CH3 or CH2（C1必须与两个CH3或CH2结合）The actived process（活化过程）C1 recognize antigen-antibody complex（C1对抗原-抗体复合物的识别）The structure of C1 and its recognition（C1的结构和识别）The activation of complemant（补体的活化） Ag-AbC1C1 C1C1C4C4a
7、C4bC2C2bC2aC4b2bC4b2b3bC4b2b3bC3C3bC3aC5C5aC5bC6,C7C6,C7C567C567C8C5678C5678nC9nC9C5678(9)nC5678(9)nThe Lysis. of Target（靶细胞的溶解）Ca+C1rC1sC1q C4C4abClassical Pathway Generation of C3-convertase Classical Pathway Generation of C3-convertase C4bMg+C4aCa+C1rC1sC1q C2C2aaC2bC4b2b is C3 convertaseClassica
8、l Pathway Generation of C5-convertaseC4bMg+C4aCa+C1rC1sC1q C2aC2baC3 C3abC4b2a3b is C5 convertase; it leads into the Membrane Attack PathwayLytic pathwayC5-activationC3b C2bC4bC5bC5a Lytic pathwayassembly of the lytic complexC5b C6C7Lytic pathway:insertion of lytic complex into cell membraneC5b C6C7
9、C8 C9C9C9C9C9C9C9C9C9ALTERNATIVEPATHWAY（替代途径）The condition of Complement activation via the alternative pathway（替代途径活化的条件）Bacteria,LPS,poly IgA or IgG4（细菌，LPS，IgA多聚体或IgG4)激活物质 细菌内毒素、酵母多糖、葡聚糖、凝集的IgA和IgG4等 BC3 C3b C3bB C3bBb C3a C3a Ba C3b D C3 C3bnBb C5a C5 C5b nC9 C8 C6,C7 C5678 C567C56789(n)LECTINP
10、ATHWAY(MBL途径）The condition of Complement activation via the lectin pathway（补体MBL途径活化的条件）Bacteria,MBL,serine protease（细菌，MBL，苏氨酸蛋白酶）The structure of MBLMBL+病原体甘露糖残基+丝氨酸蛋白酶C4C2MASPC4a + C4bC2a + C2b+C4b2bC3转化酶The differents between the three pathways（三条途径的区别）classicalalternativelectinInitiated materia
11、lAb-AgLPS,etMBLfirst activated moleculaC1C3C2,C4C composeC1C9C3,C5C9,b,dC2C9,MASPC3 convertase C4b2bC3bBbC4b2bC5 convertase C4b2b3bC3bnBbC4b2b3bfunctionSpecific immuneInnative immuneInnative immuneRegulationoftheactivationofcomplement（补体活化的调节）1.atuo-disintegration of complement（自身衰变的调节）2.Theregulati
12、onofSerum-solubleproteins（血清可溶性蛋白的调节）1) C1 inhibtior (C1INH) （C1抑制因子）抑制因子）2) C4 bingding protein,C4bp（C4结合蛋白）3) factor I4) factor H5)protein SMembrane-boundproteins（膜结合蛋白）2)DAF(decay-accelerating factor，延缓加速因子）3) HRF(homologous restriction factor，同源限制性因子)Lysisofforeignmaterial（溶解细菌）Opsonization（调理作用）Solubilizationandclearanceofimmunecomplexes（溶解和清除免疫复合物）Inflammatoryprocesses（介导炎症反应）Neutralization（中和作用）Immune regulation（免疫调节）
