《Affymetrix基因芯片产品》由会员分享，可在线阅读，更多相关《Affymetrix基因芯片产品（30页珍藏版）》请在金锄头文库上搜索。
1、Affymetrix基因芯片产品2021/9/201 Up to Up to 7,007,000,000 0,000 featuresfeatures / / chipchip5-11m5-11mRNA targetRNA targetThousands of copies of a specificThousands of copies of a specificoligonucleotide probe in oligonucleotide probe in each featureeach feature Raw data image with 1- Raw data image wit
2、h 1-6.96.9 million differentmillion differentcomplementary probescomplementary probes OligonucleotideOligonucleotideprobeprobe*1.28 cm1.28 cmHybridized probe cellHybridized probe cell*5”5”5”5”2021/9/202芯片产品分类表达谱芯片-mRNA3IVTWTMicroRNA芯片lncRNA芯片SNP/CNV芯片甲基化&转录因子芯片2021/9/203表达谱芯片3IVTHuman U133 Plus 2.0M
3、ouse U430 2.0Rat U230 2.0PrimeView&Almac XcelWTHuman/Rat/Mouse Gene1.0 STHuman/Rat/Mouse Exon1.0 STHTA2.02021/9/204表达谱芯片探针设计Exon1.0ST基因组DNA初始转录本RNA可变剪切转录本Gene1.0ST3端表达谱2021/9/205表达谱芯片实验步骤样本RNA抽提&质检cDNA合成及片段化生物素标记芯片杂交-洗脱-扫描芯片数据标准化2021/9/206Gene系列芯片TranscriptcoverageofthearrayHumanGene1.0STArrayHumanG
4、ene2.0STArrayTotalRefSeqtranscriptscovered36,07940,716NM?RefSeqcodingtranscript,well-establishedannotation32,02030,654NR?RefSeqnon-codingtranscript,wellestablishedannotation2,9675,638XM?RefSeqcodingtranscript,provisionalannotation579996XR?RefSeqnon-codingtranscript,provisionalannotation5133,428lincR
5、NAtranscripts46611,086RefSeq(Entrez)genecount21,01424,8382021/9/207EXON系列芯片DesignStatisticsSummaryProbeSets1.4millionExonClusters1millionSupportedbyPutativeFull-LengthmRNA289,961probesetsSupportedbyEnsemblTranscripts306,583probesetsSupportedbyEST665,175probesetsSupportedbyMouseorRatmRNA220,262probes
6、etsSupportedbyGenePrediction883,105probesetsProbeSelectionRegionAlongtheentirelengthofthetranscriptsProbes/ProbeSelectionRegion4BackgroundSubtractionStrategyMedianintensityofupto1,000backgroundprobeswiththesameGCcontentTotalFeaturesPerArray5,500,000InterrogatedStrandSense2021/9/208HTA2ArraycontentPr
7、oteincodingcontentNon-proteincodingcontentGenes(transcriptclusters)44,69922,829Transcripts245,34940,914Exons560,472109,930Exonclusters296,05882,444Splicejunctions(probesets)339,14602021/9/209表达谱芯片应用U133经典，相对旧，无价格优势，不做推荐Gene系列基本，仅满足一般表达量检测需求Exon系列全面，精确表达量检测HTA2最佳，最全&最精确表达量EG1.0最佳，唯一能够获得转录本表达量2021/9/2
8、010MicroRNA芯片MicroRNA 4.0microRNAPre-microRNAsnoRNA&scaRNA2021/9/2011microRNA芯片实验步骤样本RNA抽提&质检Poly(A)加尾&FlashTagBiotinHSR连接生物素标记芯片杂交-洗脱-扫描芯片数据标准化2021/9/2012microRNA4.0特点100%覆盖miRBase V20包括所有数据库记录的物种人2578小鼠1908大鼠728重复性0.95技术重复生物重复2021/9/2013lncRNA芯片Human/Mouse/Rat Gene2.0HTA2.0(Human)EG1.0-Human/Mouse
9、2021/9/2014LNCRNA芯片实验流程样本RNA抽提&质检cDNA合成及片段化生物素标记芯片杂交-洗脱-扫描芯片数据标准化2021/9/2015SNP/CNV芯片SNP6.0CytoScan750KCytoScanHDOncoScan订制芯片2021/9/2016Cyto芯片适用样本细胞组织血液唾液骨2021/9/2017CytoHD2021/9/20182021/9/20192021/9/2020纯合合杂合合2021/9/2021父本父本母本母本子代子代2021/9/20222021/9/2023Cyto系列芯片价值2021/9/2024甲基化芯片 Human Promoter1.0 R2021/9/20252021/9/2026研究甲基化意义2021/9/20272021/9/20282021/9/2029Promoter1.0R实验流程2021/9/2030
