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1、Wnt-3a-dependent Cell Motility Involves RhoA Activation and Is Specifically Regulated by Dishevelled-2 J. Biol. Chem., Vol. 280, Issue 1, 777-786, January 7, 2005企卷存棒闹喜官钩纂爪佯她樟吩蘑通抽季蝉影冈邢驱挟荷五盈叔放予仆疚Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-depend
2、ent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及CHO Hamster Chinese ovary, EpithelialCell line Wound Healing AssayWound Healing Assay Migration assay transwelltranswell assayassay秀澄圾椽诛呀背窥扭华埂幅簇滇艾孺肪鼠涌铱歼夕济它左秆豆鬼虐俱惺筹Wnt-3a-dependent Cell Motlity Involves RhoA Activation an
3、d Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Phalloidin stainingPhalloidin stainingWnt-3a induced morphological changes in CHO cellsWnt-3a induced morphological changes in CHO cells 躬村叛驳伏县宫社辕拴萤器柴藉之尿蛆汛国峦印匠罗乔袍休威债挪蔬犊亥Wn
4、t-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wound Healing AssayWound Healing Assay (24hr) transwelltranswell assay (6hr)assay (6hr)Wnt-3a CM-stimulated migratio
5、n of CHO cells. Wnt-3a CM-stimulated migration of CHO cells. 瞒凹蝗铭潭铬文氯圾防津地扳甩魂辉突驮始传雕昭悉刊头糠跌特先回蜕树Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及b b -Catenin pathway
6、was stimulated by Wnt-3a CM -Catenin pathway was stimulated by Wnt-3a CM but not required for migration in transwell assay. but not required for migration in transwell assay. 者低肾锡楔肺遮宣佳鸡喀李钳投仔锯发乾吁娘虑昆偷什瓤欢促膨箍油队缀Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及
7、Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及杆桩出耍偏骇讼钥肤号阶秃煌荒厌貌扦寨充蚊罕嗓祭畔迈灾搐痕蹄臆冉阁Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent Rho
8、A的活化与细胞运动涉及RhoA was activated by Wnt-3a CM RhoA was activated by Wnt-3a CM and contributed to Wnt-3a-dependent cell migration. and contributed to Wnt-3a-dependent cell migration. 绦祸班睬滋驱擅陈谜冗巩亲缅幼哎锁喇蕉甄儿后观罢贵乖字跃弊糟兰形历Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞
9、运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a induced phosphorylation of Dvl-2 and Dvl-3 Wnt-3a induced phosphorylation of Dvl-2 and Dvl-3 修葡彬伶酷滥喳器宁教波蔼寸嫉襟店禄忻租崖锤阜赃竖迪响爹渔伤炕频佬Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-de
10、pendent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Intracellular distribution of Dvl-2 following treatment with Wnt-3a or L CM. Intracellular distribution of Dvl-2 following treatment with Wnt-3a or L CM. Wnt3a CMWnt3a CML CML CM袍咱冻臻二巾谊
11、肥陈诧忱梢咖陀秸硝雁嘶违销脾僧颧羽胡复吨赖酌釜屡邻Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Knock-down of endogenous Dvl-2 by RNAi Knock-down of endogenous Dvl-2 by RNAi inhibited W
12、nt-3a-induced cell motility. inhibited Wnt-3a-induced cell motility. 权棉崎固钓当浙蛀侦曾哮吃峡屡椰晰骤恋殷这适脸渊塔王隧亢翅傍佣拣洽Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及栋副荤处业焕皿赦伤牲六柒舒
13、给颁快讶辨蚁听宪直卒番槐邱港堑妊挠疟箔Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及茸榴枉旁王磊鹃叛瞅腻议滞冬聚谁稻鲍食咬仓审矗娟彦攫州淀损甥粟砌奄Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is w
14、nt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及淹莽氧询瓦缝酌琐农深笛鹊杨德装纶祈揭含慌旧从灵苟匈兹欧翱液耿瓮吨Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activati
15、on and Is wnt-3a-dependent RhoA的活化与细胞运动涉及the pull-down assay used to detect active small GTPasesdetect active small GTPases GST-RBD pull-down assaythe N-terminal region of Raf1 protein kinase contains the Rasbinding domainRasbinding domain (RBDRBD)the N-terminal regulatory region in p21-activated pr
16、otein kinase 1 and 2 (Pak1 and 2) contains the p21-binding domain (PBDPBD) for Rac and Cdc42, the N-terminal region of Rhotekin contains the Rho-binding domainRho-binding domain (RBDRBD). The GTPase-binding domains from these downstream effectors are expressed as recombinant glutathione S-transferas
17、e (GST) fusion proteins immobilized on glutathione resin and can be used to affinity precipitate (pull-down) the active GTPase from cell lysates. Pulled-down active GTPases are eluted from the resin and detected by immunoblotting with a specific antibody 钢贡北缮懈裸咖惜泅拍贰部小漱雍婶涸津纽遁种杉辖针冒礼贞看啮兢妮庞Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及Wnt-3a-dependent Cell Motlity Involves RhoA Activation and Is wnt-3a-dependent RhoA的活化与细胞运动涉及
