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1、Muscle Tissue 肌组织肌组织Ying Ding Email:The contentn nVCD: VCD: The function of skeletal The function of skeletal musclemusclen nObservation:Observation:(1)(1)Skeletal muscle Skeletal muscle 骨骼肌骨骼肌 HE, NO.21HE, NO.21special stain shown LCT in yellow color special stain shown LCT in yellow color 荧光桃红荧光桃红
2、 NO.22NO.22(2)(2) Cardiac muscle Cardiac muscle 心肌心肌 HE, NO.19HE, NO.19special stain shown intercalated disc special stain shown intercalated disc 铁苏木素染色看闰盘铁苏木素染色看闰盘 NO.20NO.20(3)(3)Smooth muscle Smooth muscle 平滑肌平滑肌 HE, HE, NO.23NO.23n nHomework:Homework: Draw the Draw the longitudinallongitudinal
3、and cross section of and cross section of skeletal muscle fiber in high power objective lens 40, skeletal muscle fiber in high power objective lens 40, and describe its construction features.and describe its construction features.n n作业：作业：在高倍镜下描绘骨骼肌纤维的纵、横切面，并在高倍镜下描绘骨骼肌纤维的纵、横切面，并描述其结构特征。描述其结构特征。 肌组织的
4、分类、分布：肌组织的分类、分布：分类分类分布分布光镜光镜骨骼肌骨骼肌心心 肌肌平滑肌平滑肌躯干、四肢躯干、四肢心心 脏脏内脏、血管壁内脏、血管壁横纹肌横纹肌横纹肌横纹肌无横纹无横纹收缩收缩随意肌随意肌快快不持久不持久不随意肌不随意肌有节律有节律持久持久不随意肌不随意肌慢慢持久持久(1)Skeletal muscle 骨骼肌 HE NO.21n nNaked eye: The section of tongue is pink color.n n肉眼：舌头切面，染成粉红色n nLowest power objective lens 4: The most tissue is stained in
5、 pink.n n低倍镜4*：表面凹凸不平，深蓝色的为舌黏膜上皮，深面为骨骼肌，大部分染成粉红色。Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of skeletal muscle fibers. 低倍镜10*：纵、斜、横切面Skeletal muscle fibers (lower magnification)Cross sectionLongitudinal sectionBlood vessel (oblique sec
6、tion)Lower power objective lens 10: This is longitudinal section of skeletal muscle fibers. Compare the staining of muscle tissue with LCT. What are their differences? 低倍镜低倍镜10*：骨骼肌的纵切面。：骨骼肌的纵切面。 试比较其与疏松结缔组织的不同。试比较其与疏松结缔组织的不同。Skeletal muscle fibers (longitudinal section, lower magnification)(skeleta
7、l muscle fibers)loose connective tissueHigh power objective lens 40: Analyses the structure in different sections of individual skeletal muscle fiber. Multi nuclei are peripheral location just underneath of the sarcolemma, which can be seen in various sections. (the nucleus of skeletal muscle fiber)
8、(skeletal muscle fiber)a skeletal muscle fiber(cross section)myofibrils(cross section)myofibrils(longitudinal section)loose connective tissue高倍镜40*：分析不同切面的骨骼肌特征。多核，位于肌纤维的周边，紧靠肌膜内面，不同切面均可见。High power objective lens 40: In the longitudinal of skeletal muscle fiber the myofibrils are very thin parallel
9、 with the length of the cell. And identify its cross alternative light and dark band (cross stria) with the diaphragm closed down. 40*镜纵切面：与长轴平行的细丝为肌原纤维。缩小光圈区分明暗相间的横纹-明暗带。(the nucleus of skeletal muscle fiber)(skeletal muscle fiber)(loose connective tissue)cross striaHigh power objective lens 40: In
10、 the cross section of skeletal muscle fiber the myofibrils are stippled (dot) appearance. The delicate LCT surrounding each skeletal muscle fiber can be seen, which is termed endomysium. 40*横切面：肌原纤维呈细点状结构，周围包绕的细致疏松结缔组织为肌内膜。a skeletal muscle fiber (cross section)myofibrils (cross section)myofibrils (
11、longitudinal section)绘图格式绘图格式 图名，图名， 染色方法，放大倍数染色方法，放大倍数图题图题 ？标出骨骼肌标出骨骼肌某些典型结某些典型结构的名称构的名称用简单的话描述其结构特征用简单的话描述其结构特征Special stain: In order to easily define muscle tissue with CT, using this special stain to recognize them. In this staining the muscle tissue is in red, CT is in yellow. 特殊染色（荧光桃红）： 肌组织红
12、色，结缔组织黄色。Special stain:Skeletal muscle fibers (red)Loose connective tissue (yellow)(2) Cardiac muscle心肌心肌 HE NO.19; special stain, NO.20n nNaked eye: The section of the wall of heart is pink color.n n肉眼：心脏切面染成粉红色。n nLowest power objective lens 4: Move the slide you will find various sections of card
13、iac muscle fibers. n n低倍镜4*：找到不同切面的心肌纤维。通过比较观察心肌的形态结构（光镜）通过比较观察心肌的形态结构（光镜）n n肌纤维整体形态肌纤维整体形态n n肌浆（胞浆）肌浆（胞浆）n n胞核胞核n n有无特殊结构有无特殊结构n n肌纤维间的结缔组织肌纤维间的结缔组织Lower power objective lens 10: To identify various sections of the cardiac muscle fibers. Among the cardiac muscle fibers there is less amount of LCT a
14、nd rich capillaries. Look for the perfect cross and longitudinal section of cardiac muscle fibers. Then turn to Lens 40 . 10*不同切面。肌纤维之间有少量疏松结缔组织和毛细血管。找到典型的横、纵切面转到高倍镜下观察。blood vessleblood vessleLoose connective tissuecross sectionCardiac muscle (low magnification)longitudinal sectionHigh power object
15、ive lens 40: Compare to the skeletal muscle fibers. In the longitudinal of cardiac muscle fibers, which are branching and anastomosing with others, the cross-striated banding pattern is visible but not so clear than that of skeletal muscle fibers. The nucleus is located in the center, and surroundin
16、g by light cytoplasm due to without myofibrils. Try to find intercalated disc, which is darkly stained straight or step-like line crossing the fibers at interval.Cardiac muscle fibers (longitudinal section-high magnification)intercalated discThe nucleus of cardiac muscle fibercross-striated bandbloo
17、d vessle40*纵切面：心肌纤维呈短柱状，有分叉，互相吻合。明暗相间的横纹不如骨骼肌明显。核呈卵圆形，位于细胞中央，两端无肌原纤维，被浅染的胞浆包绕。在心肌纤维连接处染色较深的结构为闰盘，呈直线或者梯形。High power objective lens 40: In the cross section of cardiac muscle fibers, they are different size and appearance, the large and round appearance with or without centrally nucleus, the large an
18、d pear-like appearance with or without centrally nucleus, and the small and round shaped only with stippled appearance myofibrils.loose connective tissueblood vessela cardiac muscle fiber (cross section)40*横切面：肌纤维呈大小不一的块状。细胞核位于中央，核周围淡染。肌纤维切面有的比较大，圆形或梨形，伴或不伴细胞核，有的比较小，呈圆形，仅为斑点状的肌原纤维。Special stain: In
19、section NO.20, the intercalated disc is shown clearly. 特殊染色能清楚地显示闰盘。Intercalated discfibroblastthe nucleus of cardiac muscle fiber(3) Smooth muscle平滑肌平滑肌 HE NO.23n nNaked eye: The section of the intestine is pink color.n n肉眼：小肠切片，染成粉红色。n nLowest power objective lens 4: Look for the smooth muscle in
20、pink color.n n低倍镜4*：找到染成粉红色的平滑肌。Lower power objective lens 10:Move slide to find various sections (longitudinal, oblique and cross section) of smooth muscle fibers. 低倍镜10*：不同切面的平滑肌（纵横斜切面）。Longitudinal sectionloose connective tissueoblique sectionSmooth muscle (low magnification)High power objective
21、lens 40: In longitudinal section, The smooth muscle cell is spindle-shaped with an elongated centrally located nucleus. The narrow part of one cell lied adjacent to the broad parts of neighboring cells. 40*纵切面：平滑肌细胞长梭形，核居中，杆状、椭圆或扭曲型。一个细胞的窄部与另一细胞的宽部比邻。Smooth muscle fibers (longitudinal section-high m
22、agnification)loose connective tissuethe nucleus of smooth muscle fiberHigh power objective lens 40: The muscle fibers cut in cross-section present a mosaic of rounded or irregularly polygonal profiles with variations in diameter. Only the largest profiles contain a centrally placed cross section of the nucleus. No nucleus is found in the smaller profiles. 40*横切面：由大小不同的圆形或不规则多边形的嵌合体组成。只有大的切面才能看到位于中央的胞核，小的切面则没有。the nucleus of smooth muscle fiberSmooth muscle fibers (cross section-high magnification)
