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1、GaoyanImageJFeaturesn nRuns Everywhere:Runs Everywhere: n nImageJImageJiswritteninJava,whichallowsittorunonLinux,MacOSXandiswritteninJava,whichallowsittorunonLinux,MacOSXandWindows,inboth32-bitand64-bitmodes.Windows,inboth32-bitand64-bitmodes.n nOpen Source:Open Source: n nImageJImageJanditsanditsJa
2、vasourcecodeJavasourcecodearefreelyavailableandinthearefreelyavailableandinthepublicdomainpublicdomain. .Nolicenseisrequired.Nolicenseisrequired.n nPluginsPlugins: : n nExtendExtendImageJImageJbydevelopingbydevelopingpluginspluginsusingusingImageJsImageJsbuiltintexteditorandbuiltintexteditorandJavac
3、ompiler.Morethan500Javacompiler.Morethan500pluginsplugins areavailableareavailable. .n nAnalysis:Analysis: n nMeasurearea,mean,standarddeviation,minandmaxofselectionorentireMeasurearea,mean,standarddeviation,minandmaxofselectionorentireimage.Measurelengthsandangles.Userealworldmeasurementunitssuchas
4、image.Measurelengthsandangles.Userealworldmeasurementunitssuchasmillimeters.Calibrateusingdensitystandards.Generatehistogramsandprofilemillimeters.Calibrateusingdensitystandards.Generatehistogramsandprofileplots.plots.Installationn nLinuxn nMacOSXn nMacOS9n nWindowsWindowshttp:/imagej.nih.gov/ij/dow
5、nload.htmlDownload ImageJ 1.44 bundled with 32-bit Java 1.6.0_20 (28MB), with 64-bit Java 1.6.0_20 (24MB; requires 64-bit Windows) or without Java (3MB). OpenReads an image and displays it in a separate window. Files must be in TIFF, GIF, JPEG, DICOM, BMP, PGM or FITS format. Also opens ImageJ and N
6、IH Image lookup tables (with .lut extension). Additional file formats are supported via plugins installed in the Import submenu. Subtract backgroundMerge channelScalebarn nScalebarsshouldbepresentonallpublication/presentationimages/movies.Itsworthputtingtheminsoonerratherthanlater.Chooseastandardsca
7、lebarsizeforallyourimagesifpossibletoavoidconfusion.n nIfyouknowthesizeofafeature(previouslyappliedscalebarforinstance)youcanusethiscommandtoapplyacalibration.n nUsingthelineselectiontool,drawalingalongthelengthofthefeature/scalebar.n nRunthemenucommand“Plugins/Spatial calibration/Set scale”1.n nEnt
8、erthedimensionsoftheobject/scalebarinthe“known distance”boxandsettheunitsintheUnit of lengthbox.n nDonotcheckGlobal unlessyouwishallyourimagestohavethiscalibration!ClickOK.n nAnalyze Particles and count celln nThiscommandcountsandmeasuresobjectsinbinaryorthresholdedimages.Itworksbyscanningtheimageor
9、selectionuntilitfindstheedgeofanobject.Itthenoutlinestheobjectusingthewandtool,measuresitusingtheMeasurecommand,fillsittomakeitinvisible,thenresumesscanninguntilitreachestheendoftheimageorselection.Presstheesckeytoabortthisprocess.UseImageAdjustThresholdtothresholdanimage.Selection and cropThankyou!2011-4-192011-4-19
