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1、1134VOLUME 16 NUMBER 11 NOVEMBER 2009 nature structural & molecular biologyarticlesSevere acute respiratory syndrome (SARS) coronavirus (SCoV), the causative agent of a newly emerged disease, SARS, is an enveloped virus that contains a single-stranded, positive-sense RNA genome of about 29.7 kb1. Up
2、on infection, genome expression begins with the translation of gene 1, which constitutes the 5-end two-thirds of the viral genome, to produce two large precursor polyproteins2,3 that are proteolytically processed by two virally encoded proteinases to generate 16 mature proteins, namely nsp1 to nsp16
3、 (ref. 2). Although most of these gene-1 proteins are essential for viral RNA synthesis, some of them seem to have other biological functions47.The most N-terminal gene-1 protein, nsp1, has interesting biological functions; expressed SCoV nsp1 induces host mRNA degradation and suppresses host transl
4、ation8. The expressed nsp1 suppresses the host antiviral signaling pathways as well9. Furthermore, nsp1 suppresses host gene expression, including type I interferon (IFN) production, by promoting host mRNA degradation and host translation suppres-sion in infected cells10. The nsp1 of a closely relat
5、ed mouse hepatitis virus also suppresses host gene expression and is a viral virulence factor11. These data suggest that the SCoV nsp1-mediated suppression of host genes is important in the pathogenesis of SARS. Accordingly, a delineation of the mechanisms of the nsp1-induced suppression of host gen
6、e expression is important for providing insight into SCoV pathogenesis at the molecular level. We designed the present study to uncover the mechanism of nsp1-induced suppression of host gene expression primarily by using an in vitro system. Our data revealed that nsp1 uses a novel, two-pronged strat
7、egy to inhibit host transla-tion and gene expression.RESULTSNsp1suppressestranslationin vitroWe tested whether nsp1 protein could suppress translation in rabbit reticulocyte lysate (RRL). We expressed the full-length, wild-type nsp1 protein and its mutant form (nsp1-mt) carrying K164A and H165A muta
8、tions as glutathione S-transferase (GST)-tagged fusion proteins in Escherichia coli followed by the removal of the GST tag to gener-ate recombinant nsp1 and nsp1-mt proteins, respectively; nsp1-mt neither suppresses host translation nor promotes host mRNA degra-dation in expressing cells and infecte
9、d cells10. After incubation of the nsp1 protein with RRL at 4 C for 30 min, we added different con-centrations of capped and polyadenylated Renilla luciferase mRNA transcripts (rLuc RNA) to the mixture and incubated the samples in the presence of 35Smethionine for 30 min. In control samples in which
10、 rLuc RNA was incubated with GST and nsp1-mt proteins, rLuc activity and labeled rLuc protein abundance increased with rising mRNA concentrations. In contrast, the rLuc activity and labeled rLuc protein abundance in the nsp1-containing sample were substantially lower than in the control samples; the
11、 rLuc activity and the radio-labeled rLuc protein abundance in the presence of nsp1 were about 6%8% of the levels observed with GST or nsp1-mt (Fig. 1a,b), which clearly demonstrates that nsp1 efficiently inhibited the rLuc protein synthesis from capped rLuc RNA in RRL.Next, we examined the effect o
12、f nsp1 on translation mediated by the internal ribosome entry sites (IRES) using in vitro-synthesized, bicistronic mRNAs, Ren-HCV-FF RNA and Ren-CrPV-FF RNA, in which expression of the upstream rLuc open reading frame (ORF) was mediated by cap-dependent translation and the translation of 1Department
13、 of Microbiology and Immunology, University of Texas Medical Branch at Galveston, Galveston, Texas, USA. 2Present address: Global Centers of Excellence Program, Research Institute for Microbial Diseases, Osaka University, Osaka, Japan. 3These authors contributed equally to this work. Correspondence
14、should be addressed to S.M. (shmakinoutmb.edu).Received 6 January; accepted 21 August; published online 18 October 2009; doi:10.1038/nsmb.1680A two-pronged strategy to suppress host protein synthesis by SARS coronavirus Nsp1 proteinWataru Kamitani13, Cheng Huang1,3, Krishna Narayanan1, Kumari G Loku
15、gamage1 & Shinji Makino1Severeacuterespiratorysyndromecoronavirusnsp1proteinsuppresseshostgeneexpression,includingtypeIinterferonproduction,bypromotinghostmRNAdegradationandinhibitinghosttranslation,ininfectedcells.Wepresentevidencethatnsp1usesanovel,two-prongedstrategytoinhibithosttranslationandgen
16、eexpression.Nsp1boundtothe40Sribosomalsubunitandinactivatedthetranslationalactivityofthe40Ssubunits.Furthermore,thensp140Sribosomecomplexinducedthemodificationofthe5regionofcappedmRNAtemplateandrenderedthetemplateRNAtranslationallyincompetent.Nsp1alsoinducedRNAcleavageintemplatescarryingtheinternalr
17、ibosomeentrysite(IRES)fromencephalomyocarditisvirus,butnotinthosecarryingIRESelementsfromhepatitisCorcricketparalysisviruses,demonstratingthatthensp1-inducedRNAmodificationwastemplate-dependent.WespeculatethatthemRNAsthatunderwentthensp1-mediatedmodificationaremarkedforrapidturnoverbythehostRNAdegra
18、dationmachinery. 2009 Nature America, Inc. All rights reserved.nature structural & molecular biology VOLUME 16 NUMBER 11 NOVEMBER 2009 1135articles downstream firefly luciferase (fLuc) ORF was driven by a hepatitis C virus (HCV) IRES and a cricket paralysis virus (CrPV) IRES, respec-tively (Fig. 1c)
19、. Nsp1 efficiently suppressed the translation of the 7-methylguanosine-capped fLuc RNA, m7G-FF, as well as translation driven by both IRES elements (Fig. 1d,e). According to current models of translation initiation, the CrPV-IRES recruits the 40S ribosomal subunit in the absence of any initiation fa
20、ctors12,13, which suggested to us that nsp1 could suppress the function of the 40S subunit or block translation subsequent to the initiation step.Nsp1proteininteractswiththe40SribosomalsubunitWe tested whether nsp1 associates with the 40S ribosomal subunit and suppresses its function. We transfected
21、 human embryonic kidney (HEK) 293 cells with mRNAs encoding nsp1, nsp1-mt or chloramphenicol acetyltransferase (CAT) proteins; all of these encoded proteins carried a C-terminal myc-His tag. At 8 h after transfection, we prepared the cell extracts and performed polysome profile analysis (Fig. 2ac).
22、Expression of CAT and nsp1-mt yielded similar polysome profiles, whereas nsp1 expression resulted in the accumulation of 80S monosomes, along with a substantial reduction in the polysome abundance, in accordance with the observation that the expressed nsp1, but not nsp1-mt, suppresses host translati
23、on10. Western blot analysis of the resolved gradient fractions showed that the majority of nsp1, but not CAT and nsp1-mt, sedimented with the 40S ribosomal subunit (Fig. 2b). Likewise, only nsp1 sedimented with the 40S ribosomal sub-unit in RRL (Fig. 2df). To confirm the interaction of nsp1 with the
24、 40S ribosomal subunit, we transfected 293 cells with in vitro-synthesized mRNAs encoding nsp1-myc-His fusion protein, CAT-myc-His protein or nsp1-mt-myc-His protein and immunoprecipitated the cell extracts with antibody to myc (anti-myc). The immune complexes were washed with 0.5 M KCl, a stringent
25、, high-salt condition in which the 60S ribo-somal subunits and the translation initiation factors dissociate from the 40S subunits14. Consistent with our previous report10, the expression of nsp1 was substantially lower than that of nsp1-mt or CAT. Nevertheless, an efficient immunoprecipitation of 1
26、8S ribosomal RNA and S6 ribosomal protein, core components of the 40S subunit, with nsp1, but not with CAT and nsp1-mt (Fig. 2gi), suggested the tight association of nsp1 with the 40S ribosomal subunit. Similarly, coimmunoprecipitation analysis showed that nsp1 also inter-acted with the 40S ribosoma
27、l subunit in RRL (Supplementary Fig. 1).Nsp1inhibits80SformationThe finding that nsp1 inhibited CrPV-IRES-driven translation suggested that nsp1 could affect an event or events downstream of the 43S com-plex formation that involve the binding of eIF2GTPMet-tRNA ternary complex and other initiation f
28、actors, such as eIF3, to the 40S subunit. To test the effect of nsp1 on 48S complex formation, in which the 43S complex binds to the mRNA, and 80S monosome assembly, we examined, by sucrose gradient fractionation, the accumulation of these complexes on a radiolabeled mRNA template in the presence of
29、 nsp1. To analyze the effect of nsp1 on 80S formation, we incubated 32P-labeled rLuc RNA template with nsp1, GST or nsp1-mt in RRL in the presence of cycloheximide (CHX). CHX treatment inhibits the elongation step, but does not affect 80S monosome assembly15. We used hippuristanol, which blocks the
30、function of initiation factor eIF4A and inhibits 48S complex formation, as a control16. CHX treatment induced 80S complex accumu-lation, and hippuristanol inhibited it (Fig. 3a). Nsp1, but not GST and nsp1-mt, suppressed 80S complex formation (Fig. 3b). We tested the effect of nsp1 on 48S complex fo
31、rmation by incubating the samples with GMP-PNP, a non-hydrolyzable analog of GTP, and subsequently subjecting them to sucrose gradient centrifugation; GMP-PNP does not affect 48S complex formation, whereas it blocks GTP hydrolysis by eIF2 in eIF2-GTP-Met-tRNA complexes and inhibits the release of eI
32、F2 and subsequent joining of the 60S ribosomal subunit17. The 48S complex accumulated in the presence of GMP-PNP, but not in the presence of hippuristanol (Fig. 3c). Nsp1, nsp1-mt and GST did not suppress the 48S complex formation (Fig. 3d). These data show that nsp1 did not inhibit 48S complex form
33、a-tion, but it suppressed 60S subunit joining.Characterizationofthe48ScomplexbytoeprintinganalysisNext, we performed a toeprinting analysis, which defines the positions of stalled ribosomes on the mRNA chain18. After initial incubation of RRL with nsp1, GST or nsp1-mt in the presence of CHX or a mix
34、ture of CHX and GMP-PNP, we added rLuc RNA that had been prean-nealed with a 5-end-labeled primer to the samples. Subsequently, we subjected the samples to primer extension without extracting the 9acdebGSTrLucProteinRNA10 minGSTwtmtGSTGST1.61.41.21.00.80.60.40.20fLucfLucfLucrLucrLuc00.51.02.00.51.02
35、.00.51.02.00.51.02.00.51.02.00.51.02.0wtwtmtmt(g)(g)GSTwtmtGSTwtmt20 min30 minwtmtrLuc activity ( 108)rLuc RNA transcripts (g)Firefly luciferaseFirefly luciferaseFirefly luciferaseRenilla luciferaseRenilla luciferaseHCVCrPVIRESIRES876543210m7G-FFRen-HCV-FFRen-CrPV-FFm7G-FFRen-HCV-FFRen-CrPV-FFfLuc t
36、ranslationm7G-FFRen-HCV-FFRen-CrPV-FF0.010.050.10.250.51.02.0Figure 1 Effects of nsp1 on cap-dependent translation and IRES-mediated translation in RRL. (a) Increasing amounts of rLuc RNA were incubated in RRL in the presence of 1 g of nsp1 (wt), GST or nsp1-mt (mt) at 4 C for 30 min; the molar rati
37、os of rLuc RNA to nsp1 protein ranged from 1:10 to 1:2,000. Then the samples were incubated for 30 min at 30 C, and the rLuc enzymatic activities were measured. y axis, light units. (b) A mixture of 0.25 g of rLuc RNA and 1 g of nsp1, GST or nsp1-mt was incubated in RRL in the presence of 35Smethion
38、ine at 30 C for 10, 20 and 30 min. Translated products were analyzed on SDS-PAGE and detected by autoradiography. (c) Schematic diagram of various RNA transcripts used for d,e. (d) Increasing amounts of GST, nsp1, or nsp1-mt were incubated with RRL for 10 min at 4 C. Then, the samples were incubated
39、 at 30 C for 30 min with 0.5 g of m7G-FF RNA, Ren-HCV-FF RNA, or Ren-CrPV-FF RNA in the presence of 35Smethionine. Translated products were analyzed on SDS-PAGE and detected by autoradiography. (e) For a given RNA group, the amount of fLuc protein, determined by densitometry, in each experimental gr
40、oup was normalized to the amount of the fLuc protein in a control group, to which the purified protein was not added. For a,d, averages of three independent experiments; error bars, s.d. 2009 Nature America, Inc. All rights reserved.1136VOLUME 16 NUMBER 11 NOVEMBER 2009 nature structural & molecular
41、 biologyarticlesRNAs. In control groups, using GST and nsp1-mt, we detected a cor-rectly positioned toeprint (TP-AUG), a premature primer extension termination signal that was induced by the presence of the stalled 40S subunit. The toeprint was 1517 nt downstream from the translation initiation codo
42、n in the presence of GMP-PNP and CHX, which allow 48S complex formation but not 60S joining (Fig. 4a). In the presence of CHX, the control groups showed an extra band approximately 3 nt downstream from TP-AUG, which was most probably generated by the joining of the 60S subunits to the 40S complex po
43、sitioned at the AUG codon (Fig. 4a). Consistent with the observation that nsp1 suppressed the 80S complex formation (Fig. 3b), nsp1 caused a clear reduction in the amount of the toeprint for the 80S complex (Fig. 4a). Compared to the observations in the control groups, the intensity of the TP-AUG ba
44、nd was also lower in the sample containing nsp1 (Fig. 4a), which suggests that nsp1 impaired start-codon recog-nition. Unexpectedly, we detected several more bands, both upstream and downstream of the TP-AUG, in the sample incubated with nsp1, but not in controls; a longer exposure of the CHX-treate
45、d sample clearly showed the same signals (data not shown). These data suggest that nsp1 induced the modification of rLuc RNA at several discrete sites, which blocked cDNA elongation on these modified RNAs.Nsp1rendersthecappedmRNAtranslationallyinactiveWe performed primer extension analyses to determ
46、ine whether nsp1 indeed induced template RNA modification. After incubation of rLuc RNA with GST, nsp1 or nsp1-mt in RRL, as described above, we extracted the RNA and performed primer extension analysis using the same 5-end labeled primer. In addition to the full-length primer extension product, onl
47、y samples incubated with nsp1 showed sev-eral premature primer-extension termination products, all of which corresponded to those detected in the toeprinting analysis performed in the presence of nsp1 (Fig. 4b). Northern blot analysis of rLuc RNA, extracted after incubation with nsp1 in RRL under th
48、e same conditions, did not show significantly less of the full-length rLuc transcript than the control groups (Supplementary Fig. 2). These data demonstrate that nsp1 induced the modification of capped rLuc RNA at several sites in the 5 region without inducing extensive template RNA degradation.Cent
49、rifugation40SdghiefabcCAT28S18Swt28S18Smt28S18Swt28S18Smt28S18SGSTProbe293MockCATwtmt293MockCATwtmtCATwtmt28S18S28S18SWBS6S6Relative bandintensity of 18S2.01.51.00.50HisCATnsp11 2 3 4 5 6 7 8 9FractionsFractionsIP: anti-mycIP: anti-myc1 2 3 4 5 6 7 8 9FractionsFractions1 2 3 4 5 6 7 8 91 2 3 4 5 6 7
50、 8 91 2 3 4 5 6 7 8 91 2 3 4 5 6 7 8 9FractionsFractionsO.D. 254 nmO.D. 254 nmO.D. 254 nm40S40SPolysomesPolysomesPolysomesCentrifugationCentrifugationCentrifugationCentrifugationCentrifugationFigure 2 Binding of nsp1 to the 40S ribosomal subunits. (ac) CAT (a), nsp1 (b) or nsp1-mt (c) RNAs were tran
51、sfected into 293 cells. Cell extracts were prepared 8 h after transfection and subjected to polysome profile analysis (top panels). CAT, nsp1 (wt) and nsp1-mt (mt) in each fraction were detected by western blot analysis using antibody to myc (middle panels). Bottom panels show rRNAs in each fraction
52、. (df) After incubation of the mixture of 0.25 g of rLuc RNA and 1 g of nsp1, GST or nsp1-mt in RRL for 30 min at 30 C, the samples were separated on 10%50% sucrose gradient. GST, nsp1 (wt) and nsp1-mt (mt) in each fraction were detected by western blot analysis using anti-GST antibody and anti-nsp1
53、 (ref. 8) antibody. The locations of GST (d), nsp1 (e) or nsp1-mt (f) in fractions are shown (all top panels). The bottom panels show rRNAs. (gi) 293 cells were transfected with CAT RNA (CAT), nsp1 RNA (wt), or nsp1-mt RNA (mt). Cell extracts were prepared 8 h after transfection and subjected to imm
54、unoprecipitation using anti-myc antibody. Extracted RNAs from the immunoprecipitates were separated by agarose gel electrophoresis, and rRNAs were detected by northern blot analysis (g). The immunoprecipitated proteins were examined by western blot analysis using anti-S6 antibody (40S subunit specif
55、ic) and anti-His antibody for CAT, nsp1 and nsp1-mt (h). Panel I represents the values, each of which was obtained by dividing the abundance of the immunoprecipitated 18S rRNA with the immunoprecipitated CAT protein, nsp1 protein or nsp1-mt protein in an arbitrary scale.4,000abcd80S80S48S48SCHXCHX +
56、 GSTCHX + wtCHX + mtGMP-PNP + GSTGMP-PNP + wtGMP-PNP + mtGMP-PNPGMP-PNP + hipp.CentrifugationCentrifugationCentrifugationCentrifugationCHX + hipp.3,0002,0001,0003,0002,5002,0001,5001,000500032P c.p.m. mRNA32P c.p.m. mRNA3,0002,5002,0001,5001,000500032P c.p.m. mRNA2,5002,0001,5001,000500032P c.p.m. m
57、RNA0Fractions15101520Fractions15101520Fractions15101520Fractions15101520Figure 3 Ribosome binding assays of radiolabeled rLuc RNA. Representative data from three independent experiments. (a) rLuc RNA was incubated in RRL in the presence of CHX only or a mixture of CHX and hippuristanol (hipp). The p
58、ercentages of the rLuc RNA bound in 80S complexes were, for CHX, 17.4%; CHX + hipp., 1.0%. (b) rLuc RNA was incubated in RRL with GST, nsp1 (wt) or nsp1-mt (mt) in the presence of CHX. The percentages of the rLuc RNA bound in 80S complexes were, for GST, 13.3%; nsp1, 5.4%; nsp1-mt, 14.8%. (c) rLuc R
59、NA was incubated in RRL in the presence of GMP-PNP or a mixture of GMP-PNP and hipp. The percentages of the rLuc RNA bound in 48S complexes were, for GMP-PNP, 11.7%; GMP-PNP + hipp., 2.9%. (d) rLuc RNA was incubated in RRL with GST, nsp1 (wt) or nsp1-mt (mt), in the presence of GMP-PNP. The percenta
60、ges of the rLuc RNA bound in 48S complexes were, for GST, 13.1%; nsp1, 12.1%; nsp1-mt, 12.0%. 2009 Nature America, Inc. All rights reserved.nature structural & molecular biology VOLUME 16 NUMBER 11 NOVEMBER 2009 1137articlesTo further characterize the nsp1-induced template RNA modification, we incub
61、ated cap-labeled rLuc RNA and 3-end-labeled rLuc RNA, inde-pendently, with nsp1 in RRL in the presence of CHX and GMP-PNP; GST and nsp1-mt served as controls. After incubation, we analyzed the extracted RNAs on a 5% sequencing gel. The amount of intact cap-labeled rLuc RNA remaining after incubation
62、 with nsp1 was clearly lower than that in the control incubations (Fig. 5a,b), demonstrating that the 5-end region of the capped rLuc RNA was removed in the presence of nsp1. Incubation of the 3-end-labeled rLuc RNA with nsp1 resulted in the appearance of an extra band that migrated slightly faster
63、than the intact RNA (Fig. 5a). The total radioactivity of the intact 3-end labeled rLuc RNA and the fast-migrating RNA band in nsp1-treated samples was simi-lar to that of the intact 3-end labeled rLuc RNA in the controls (Fig. 5b). To determine the approximate size of the fast-migrating band, we in
64、cu-bated 3-end-labeled, non-polyadenylated rLuc RNA with nsp1 in RRL; we used non-polyadenylated rLuc RNA because some of the polyadenylated rLuc preparations had mRNA species with shorter poly(A) tails, which interfered with the positive identification of the truncated RNA products in the gel. We o
65、bserved two main fast-migrating bands, and their sizes were approximately 20 to 40 nt shorter than the full-length RNA template (Supplementary Fig. 3). These data demonstrate that nsp1 induced the removal of the 5 end, but not the 3 end, from the rLuc RNA.Next, we tested the translational competence
66、 of the rLuc RNA that had undergone the nsp1-induced modification. After incubation of the rLuc RNA in RRL in the presence of nsp1, GST or nsp1-mt, we added the same amount of CAT RNA to each sample as an internal control RNA, extracted the RNAs and performed a second in vitro translation reac-tion
67、without adding nsp1, GST or nsp1-mt. We performed this analysis to examine the translational competence of RNAs that were extracted from the first in vitro translation reaction. As expected, nsp1 suppressed the translation of rLuc RNA in the initial translation (Fig. 5c,d). The rLuc RNA that had und
68、ergone the nsp1-mediated modification was not efficiently translated into rLuc protein (Fig. 5c,d), which demonstrated that the modified rLuc RNA was translationally inactive.Nsp1inducesRNAcleavageinanIRES-drivenmRNAtemplateIn addition to cap-dependent translation, nsp1 also suppressed transla-tion
69、mediated by IRES elements derived from HCV and CrPV (Fig. 1). To address the question whether the nsp1-induced template RNA modifi-cation is specific for cap-dependent RNA template, we tested whether nsp1 induced the modification of IRES in RNA templates carrying HCV, CrPV and encephalomyocarditis v
70、irus (EMCV)-derived IRES elements. We incubated the RNA transcripts Ren-HCV-FF, Ren-CrPV-FF and Ren-EMCV-FFa bicistronic RNA carrying EMCV IRES between the upstream rLuc ORF and the downstream fLuc ORF (Fig. 6a)in RRL in the presence of nsp1, GST or nsp1-mt. After incubation, we extracted the RNAs a
71、nd analyzed them by northern blot (Fig. 6). Nsp1 did not induce any cleavage of Ren-HCV-FF and Ren-CrPV-FF (Fig. 6b and Fig. 6c, GabRNAAUG start site(+1 to +3)GSTwtmtGSTwtmtA TCHX5 endTP-AUGRibosome pausedalong 5 UTRCHX +GMP-PNPC123*4567GAUG start site(+1 to +3)GSTwtmtGSTwtmtRNAA TCHX5 endCHX +GMP-P
72、NPC1234567Figure 4 Toeprinting and primer extension analyses. (a) Toeprinting. Samples shown in lanes 24 and 57 were incubated with CHX and a mixture of CHX and GMP-PNP, respectively, and toeprinting analysis was performed in the presence of GST (lanes 2 and 5), nsp1 (lanes 3 and 6) or nsp1-mt (lane
73、s 4 and 7). Lane 1, the primer extension products using the rLuc RNA that was hybridized with a 5-end labeled primer; 5 end, the primer extension product that was extended to the 5 end of rLuc RNA; UTR, untranslated region; TP-AUG, a correctly positioned toeprint. Arrowhead and asterisk represent a
74、signal generated by the joining of the 60S subunits to the 40S complex positioned at the AUG codon. A dideoxynucleotide sequence of the rLuc gene generated with the same primer was run in parallel (left four rows).Translation initiation codon is shown at the left side of the gel. (b) Primer extensio
75、n analysis. RRL was incubated with GST (lanes 1 and 4), nsp1 (lanes 2 and 5), or nsp1-mt (lanes 3 and 6) in the presence of CHX (lanes 13) or a mixture of CHX and GMP-PNP (lanes 46) for 5 min at 30 C. Then rLuc RNA was added to each sample, and the samples were incubated for another 10 min at 30 C.
76、The RNAs were extracted and subjected to primer extension analysis. Lane 7, the primer extension products using the rLuc RNA that was hybridized with a 5-end labeled primer.Cap-labeledGSTrLucabcd28S18S1.2Relative band intensityrLuc RNA1.00.80.60.40.201.21.4rLuc translation1.00.80.60.40.20wtmtGSTwtmt
77、GSTwtmtGSTrLucrLucCAT1st2nd1st2stwtmtGSTwt*mt3-labeledCap-labeled3-labeledFigure 5 Nsp1-induced modification of rLuc RNA in RRL. (a) Cap-labeled rLuc RNA and the 3-end-labeled rLuc RNA were incubated with GST, nsp1 (wt) or nsp1-mt (mt) in RRL for 15 min at 30 C in the presence of CHX and GMP-PNP. RN
78、As were extracted, resolved on a sequencing gel and visualized by autoradiography. Asterisk, a signal that migrated slightly faster than the full-length rLuc in the sample incubated with nsp1. The 28S and 18S rRNAs, detected by ethidium bromide staining, are shown as loading controls. (b) Densitomet
79、ric determination of rLuc RNA band intensities in a, normalized to the value of the GST-incubated sample. For the 3-labeled rLuc RNA in the nsp1 sample, graph shows a sum of the signal intensities of the full-length rLuc RNA band and the band marked by the asterisk in a. (c) RRL was incubated with G
80、ST, nsp1 (wt), or nsp1-mt (mt) for 10 min at 4 C. Then rLuc RNA was added and samples incubated at 30 C for 20 min in the presence of 35Smethionine (1st translation). Aliquots were analyzed by SDS-PAGE. After addition of the same amount of CAT RNA to each sample, RNAs were extracted and translated u
81、sing fresh RRL in the presence of 35Smethionine (2nd translation), and the samples were analyzed on a 12.5% SDS-PAGE gel. (d) Quantitation of rLuc protein abundances in the 1st and 2nd translation reactions in experiment in c. For b,d, averages of three independent experiments; error bars, s.d. 2009
82、 Nature America, Inc. All rights reserved.1138VOLUME 16 NUMBER 11 NOVEMBER 2009 nature structural & molecular biologyarticlesrespectively). In contrast, nsp1 did induce cleavage of Ren-EMCV-FF (Fig. 6d). A cleaved RNA fragment, detected by a probe that binds to the rLuc ORF, migrated similarly to co
83、ntrol RNA1, carrying the rLuc ORF and EMCV IRES (Fig. 6d), and the size of the other RNA frag-ment, detected by a probe that binds to the fLuc ORF, was similar to that of the fLuc ORF in Ren-EMCV-FF. These data suggest that nsp1 induced the cleavage at or near the 3 region of the EMCV IRES in Ren-EM
84、CV-FF (Fig. 6d). These results demonstrate that nsp1 induced a template-dependent RNA cleavage in IRES-driven RNAs and that the nsp1-induced RNA modification was not specific for capped RNA template.Thensp1-40SsubunitcomplexexertstemplateRNAmodificationTo assess the biological significance of nsp1 b
85、inding to 40S sub- units, we examined whether nsp1 could modify the rLuc RNA without ribosomes. After pelleting the ribosomes in RRL by centrifugation, we incubated the ribosome-free supernatant with the cap-labeled rLuc RNA and nsp1 in the presence of CHX and GMP-PNP; GST and nsp1-mt served as cont
86、rols. Gel electrophoresis analysis of the extracted RNAs showed that the amounts of the cap-labeled rLuc RNA were similar among the three samples (Fig. 7a,b). To determine whether the rLuc RNA that had been incubated with nsp1 in the absence of the ribosomes was translationally competent, we incubat
87、ed the capped rLuc RNA with nsp1, GST or nsp1-mt in the ribosome-free RRL. As expected, rLuc protein synthesis did not occur in the ribosome-free lysate (Fig. 7c, top panel). After incubation, we added the same amount of CAT RNA to the samples, extracted the RNAs and subjected them to in vitro trans
88、lation without adding nsp1, GST or nsp1-mt. The rLuc RNA that had been incubated with nsp1 in the ribosome-free RRL was translationally competent in the second in vitro translation assay (Fig. 7c, middle panel). These data suggest that the 40S subunitbound nsp1 modified and inactivated the rLuc RNA.
89、We further tested whether the association of rLuc RNA with the 43S complex was required for nsp1-mediated template RNA modification by incubating the cap-labeled rLuc RNA with nsp1, GST or nsp1-mt in RRL in the presence of hippuristanol. Nsp1 did not affect the amount of the labeled RNA in the prese
90、nce of hippuristanol (Fig. 7d), leading us to suggest that the modification of the capped RNA requires the association of template RNA with the nsp140S complex.Thensp1-40SsubunitcomplexistranslationallyinactiveNsp1 suppressed translation mediated by the CrPV IRES (Fig. 1) but did not induce cleavage
91、 of the Ren-CrPV-FF RNA (Fig. 6b). These data suggest that, in addition to modifying template RNA, the associa-tion of nsp1 with 40S ribosomal subunit also inactivated the transla-tional function of 40S ribosomes. To further examine this possibility, we subjected Ren-CrPV-FF RNA to in vitro translat
92、ion in RRL in the presence of nsp1, GST or nsp1-mt. After incubation, we analyzed aliquots of the samples by SDS-PAGE. As expected, nsp1, but not GST or nsp1-mt, suppressed both cap-dependent and IRES-dependent translation (Fig. 8a). We added the same amount of the CAT RNA to the remaining samples,
93、extracted RNAs and performed a second in vitro translation analysis without adding nsp1, GST or nsp1-mt (Fig. 8a,b). The cap-dependent rLuc gene translation, from Ren-CrPV-FF RNA that was exposed to nsp1 in the first in vitro translation reaction, was inhibited, but the IRES-mediated fLuc gene trans
94、la-tion was not inhibited. Thus, nsp1 suppressed CrPV IRES-mediated translation in the first in vitro translation reaction but did not induce the modification that inactivated the translational activity of CrPV IRES in the second in vitro translation assay. As with cap-labeled rLuc RNA (Fig. 5), inc
95、ubation of cap-labeled Ren-CrPV-FF RNA with nsp1 resulted in the removal of the 5 region from this RNA (Fig. 8c). We interpreted these results to mean that binding of nsp1 to 40S ribosomal subunits inactivated the translational functions of 40S ribosomes, leading to the inhibition of CrPV IRESdriven
96、 translation of Ren-CrPV-FF. Collectively, these data show that nsp1 suppressed translation by inactivating the translational functions of 40S ribos-omal subunits and modifying the template RNA.Ren-EMCV-FFRenilla luciferaseFirefly luciferaseEMCVIRESRNA1abdcGSTwtmtRNARNARNA1RNA2MockGSTwtmtMockGSTwtmt
97、GSTfLucfLucrLuc probefLuc probewtmtRNARNA2FullFullFullFigure 6 Nsp1 induces cleavage at the IRES in Ren-EMCV-FF RNA. (a) Schematic diagram of Ren-EMCV-FF RNA and control RNAs RNA1 and RNA2. (b,c) After incubation of RRL with GST, nsp1 (wt), or nsp1-mt (mt) for 10 min at 4 C, Ren-HCV-FF RNA (b) or Re
98、n-CrPV-FF RNA (c) was added to each sample and incubated at 30 C for 25 min. Then RNAs were extracted and analyzed by northern blot using a digoxigenin-labeled probe that binds to the fLuc ORF. RNA lane in b, the untreated Ren-HCV-FF RNA; fLuc lane in c, the RNA encoding fLuc ORF; RNA, the untreated
99、 Ren-CrPV-FF RNA. (d) RRL was incubated with GST, nsp1 (wt), or nsp1-mt (mt) for 10 min at 4 C. Ren-EMCV-FF RNA was added to each sample and incubated at 30 C for 10 min. RNAs were extracted and analyzed by northern blot using a probe that binds to the rLuc ORF (rLuc probe) or fLuc ORF (fLuc probe).
100、 Samples in the first three lanes, including untreated Ren-EMCV-FF (RNA), RNA1 and RNA2, served as size markers.Cap-labeledGSTrLuc1.2Relative band intensityof rLuc RNA1.00.80.60.40.20GSTrLuc1st2ndrLucCATGSTcdbaCap-labeledwtwtmtmtGSTrLucwtmtwtmtFigure 7 Importance of ribosomes for the function of nsp
101、1. (a) Cap-labeled rLuc RNA was incubated with GST, nsp1 (wt), or nsp1-mt (mt) in ribosome-free RRL for 15 min at 30 C in the presence of CHX and GMP-PNP. RNAs were extracted, resolved on a sequencing gel and visualized by autoradiography. (b) The band intensity of rLuc RNA shown in a was determined
102、 by densitometric analysis. Mean of three independent experiments. (c) Experiments performed as described in Figure 5c, except that ribosome-free RRL was used for the initial translation reaction. (d) Cap-labeled rLuc RNA was incubated with GST, nsp1 (wt), or nsp1-mt (mt) in RRL in the presence of h
103、ippuristanol. RNAs were extracted, resolved on a sequencing gel and visualized by autoradiography. 2009 Nature America, Inc. All rights reserved.nature structural & molecular biology VOLUME 16 NUMBER 11 NOVEMBER 2009 1139articlesDISCUSSIONHost translational suppression, often detected in virus-infec
104、ted cells, is an effective viral defensive strategy for suppressing host innate immune responses and freeing host translational machinery for viral-specific gene expression. Although many viral proteins that suppress host translation have been identified1922, SCoV nsp1 (ref. 8) and several herpesvir
105、us proteins2326, including herpes simplex virus-1 vhs protein27, are known to promote destabilization of host mRNAs and suppress host transla-tion. Vhs protein, which has a sequence similarity to known RNases, is indeed an RNase28, induces RNA cleavage in RRL29 and degrades both host and viral mRNAs
106、30. Unlike vhs, SCoV nsp1 has similarity in sequence and structure with no known proteins. The biological activi-ties of SCoV nsp1 also substantially differed from those of vhs, as nsp1 inactivated the translational functions of the 40S ribosomal subunit and modified RNA transcripts only in the cont
107、ext of its association with the 40S subunit. The strategy that SCoV nsp1 has evolved to bind to 40S subunits to exert its function seems to be an excellent one for blocking translation of both capped mRNAs and IRES-containing mRNAs, and for efficiently accessing host mRNAs, including those involved
108、in host innate immune functions, and inactivating them.By binding to the 40S ribosomal subunits and inactivating them, SCoV nsp1 efficiently suppressed cap-dependent and IRES-mediated translation, including translation mediated by CrPV IRES. In contrast to SCoV nsp1, CrPV IRES-mediated translation i
109、s relatively insen-sitive to Drosophila Reaper, a potent apoptosis inducer, which also inhibits translation by binding directly to the 40S subunit31.Further studies are needed to determine how the binding of nsp1 to 40S ribosomal subunits inactivates their translational function. Using rLuc RNA as a
110、 template, we showed that the formation of the 80S com-plex, but not the 48S complex, was inhibited in the presence of nsp1. Because the nsp140S complex also modified the 5 region of the rLuc RNA, it is possible that the nsp1-mediated RNA modification affected the formation of 48S and/or 80S complex
111、es on these modified RNA templates (Fig. 3). We obtained somewhat conflicting results, which showed that nsp1 did not suppress the 48S assembly on rLuc RNA (Fig. 3d) but that the 48S TP-AUG signal was reduced in the presence of nsp1 (Fig. 4a). These data may imply that some of the multiple toeprints
112、 upstream of the TP-AUG, observed in nsp1-treated sample, represent arrested 48S translation intermediates as a result of nsp1 inactivating the function of 40S ribosomal subunit, leading to ineffi-cient start site recognition; these arrested 48S translation intermediates could contribute to the sign
113、al observed for 48S complex formation (Fig. 3d). Furthermore, the nsp1-induced RNA modification alone cannot fully account for the substantially reduced luciferase protein synthesis from capped rLuc RNA (Fig. 1), highlighting the contribu-tion of the translation inhibition function of nsp1 to the su
114、ppression of reporter protein expression in RRL. To clarify the exact step in the translation of capped mRNAs that nsp1 inhibits, it would be useful to isolate an nsp1 mutant that retains the 40S subunit inactivating func-tion but lacks the RNA modification function and test the effect of this mutan
115、t on formation of 48S and 80S complexes.In addition to the modification of the 5 region of capped rLuc RNA (Fig. 5), Nsp1 also inactivated the translational competence of capped CAT or fLuc RNAs (data not shown), suggesting that nsp1 could modify capped RNAs in general. Nsp1 induced a substantial re
116、duction in the amount of full-length cap-labeled rLuc RNA (Fig. 5a), which suggested that the very 5 end of the rLuc RNA, including the 7-methylguanosine cap and the radiolabeled phosphate group, may have been removed. Primer extension analysis of the rLuc RNA that had been incubated with nsp1 (Fig.
117、 4b) revealed that nsp1 caused the modification of several different regions at the 5 end of rLuc RNA. An RNA signal that migrated slightly faster than the full-length rLuc RNA was detected after incubation of the 3-end labeled rLuc in the presence of nsp1 (Fig. 5a). Analysis of the nsp1-treated non
118、-polyadenylated rLuc RNA further showed that the fast-migrating species of rLuc RNA shown in Fig. 5a consisted of at least two bands that were roughly 20 to 40 nt shorter than the full-length rLuc RNA template (Supplementary Fig. 3). These data point to a possibility that the several premature prime
119、r extension termination products, which were obtained using nsp1-treated rLuc RNA (Fig. 4b), were derived from rLuc RNA species lacking different regions from their 5 ends. Because nsp1 induced an internal RNA cleavage in Ren-EMCV-FF (Fig. 6), we suspect that nsp1 also triggered a similar endonucleo
120、lytic RNA cleavage in the 5 region of rLuc RNA. We also noted that the amounts of the full-length primer extension product obtained from rLuc RNA that had been incu-bated with nsp1 were not substantially different from the corresponding signal obtained from the control group (Fig. 4b). These data im
121、ply that nsp1 induced the removal of the entire or a portion of the cap structure without affecting the first 5-end nucleotide from the full-length rLuc RNA. Probably the putative nsp1-induced endonucleolytic cleavage and decapping activities both contributed to the translational inactivation of nsp
122、1-treated rLuc RNA.Nsp1 induced RNA cleavage in the EMCV IRES of Ren-EMCV-FF, but it did not induce the RNA cleavage in Ren-CrPV-FF and Ren-HCV-FF RNAs, revealing the template-dependent nature of nsp1-induced RNA cleavage. Furthermore, these data demonstrated that nsp1-induced RNA modification was n
123、ot limited to cap-dependent RNA templates. Because the translation initiation factors eIF4G and eIF4A are required for cap-dependent and EMCV IRES-mediated translation but not for translation driven by the IRES elements of HCV and CrPV32, these factors may be involved in the induc-tion of nsp1-media
124、ted endonucleolytic cleavage of template RNAs. Alternatively, nsp1 may recognize putative unique RNA element(s), present in EMCV IRES and absent in HCV and CrPV IRES elements, to induce the RNA cleavage in the EMCV IRES. Further studies GSTfLucabcfLucfLucrLuc1st2ndrLucrLuc1.81.61.41.21.00.80.60.40.2
125、Full0CATwtmtGSTwtmtGSTLuc translationCap-labeledwtmtFigure 8 Nsp1 inactivates the translational function of 40S ribosomal subunit. RRL was incubated with GST, nsp1 (wt), or nsp1-mt (mt) for 10 min at 4 C. Ren-CrPV-FF RNA was added to each sample and incubated at 30 C for 25 min in the presence of 35
126、Smethionine. Aliquots of the samples were used for SDS-PAGE (a, 1st). RNAs were extracted from the remaining samples after addition of the same amount of CAT RNA to each sample. Experiments were performed as described in Figure 5c, except that Ren-CrPV-FF was used as the template RNA. rLuc and fLuc
127、are expressed by cap-dependent and CrPV IRES-mediated mechanisms, respectively (a, 2nd). (b) Experiments described in a were repeated three times, and the amounts of fLuc and rLuc proteins in the 2nd translation reaction are shown. Error bars, s.d. (c) Cap-labeled Ren-CrPV-FF RNA was incubated with
128、GST, nsp1, or nsp1-mt in RRL for 15 min at 30 C in the presence of CHX and GMP-PNP. RNAs were extracted, resolved on a sequencing gel and visualized by autoradiography. 2009 Nature America, Inc. All rights reserved.1140VOLUME 16 NUMBER 11 NOVEMBER 2009 nature structural & molecular biologyusing vari
129、ous RNAs carrying different types of IRES elements will be necessary to distinguish between these two possibilities.Two different mechanisms are conceivable for how the nsp1-40S ribos-ome complex induces the RNA modification at the 5-end region of capped rLuc RNA and at the EMCV IRES of Ren-EMCV-FF
130、RNA. One is that the activated nsp1, induced by the binding of nsp1 to the 40S subunits, modifies these RNAs. Another possibility is that binding of nsp1 to the 40S subunits induces the activation or recruitment of a host factor(s) that modifies the template RNAs. It is also possible that some ribos
131、ome- associated factors are involved in nsp1-induced RNA modification. Indeed, host endonucleases can destabilize host mRNAs during translational elonga-tion in polysomes33,34. Because nsp1 modified the rLuc RNA during transla-tion initiation (Figs. 35), the nsp1-mediated modification of the 5 regio
132、n of the capped RNA presents a novel strategy for modifying capped RNAs.The expression of nsp1 in mammalian cells caused both host mRNA degradation and host translation inhibition8. Because host mRNAs that are not involved in active translation are delivered to host mRNA degradation pathways35, we h
133、ypothesize that host capped mRNAs that underwent the nsp1-mediated translation inhibition and modifica-tion in expressing cells and SCoV-infected cells are delivered to the host mRNA degradation machinery36 and efficiently degraded.Efficient SCoV-specific gene expression occurs in infected cells, in
134、 which nsp1 suppresses host gene expression10, which implies that SCoV gene expression is somehow immune to the nsp1-induced sup-pression of gene expression in infected cells. Like many host mRNAs, coronavirus mRNAs are capped and polyadenylated37. Several mecha-nisms are conceivable for an apparent
135、 selective immunity of SCoV gene expression to the nsp1-induced translational suppression. One is that the SCoV-specific translation occurs in selected subcellular com-partments, where the localization and the access of nsp1 is limited. Another possibility is that viral or host-derived protein(s) se
136、lectively bind to SCoV mRNAs and facilitate SCoV-specific translation in the presence of nsp1. Alternatively, the efficient accumulation of SCoV mRNAs outcompetes the nsp1-induced translational suppression.METhODSMethods and any associated references are available in the online version of the paper
137、at http:/ Supplementary information is available on the Nature Structural & Molecular Biology website.ACKNoWLedGMeNtSWe thank P. Sarnow (Stanford University) for the CrPV1.1 plasmid, S. Lemon (University of Texas Medical Branch at Galveston) for the pRC22F plasmid and J. Pelletier (McGill University
138、) and J. Tanaka (University of the Ryukyus) for hippuristanol. This work was supported by US National Institutes of Health Public Health Service grant AI72493. W.K. and C.H. were supported by the James W. McLaughlin Fellowship Fund.AUtHoR CoNtRIBUtIoNSW.K., C.H. and K.G.L. generated the reagents. W.
139、K. and C.H. performed the experiments. W.K., C.H., K.N. and S.M. designed the study. W.K., K.N. and S.M. wrote the paper.Published online at http:/ Reprints and permissions information is available online at http:/ Poon, L.L., Guan, Y., Nicholls, J.M., Yuen, K.Y. & Peiris, J.S. The aetiology, origin
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160、 Res. 117, 1737 (2006).articles 2009 Nature America, Inc. All rights reserved.nature structural & molecular biologydoi:10.1038/nsmb.1680ONLINEMEThODSPlasmid construction. We cloned pST-fLuc by inserting the cDNA encoding the entire fLuc ORF region from the pGL3 control vector (Promega) into pSPT18 (
161、Roche). We cloned the PCR-amplified full-length nsp1 and nsp1-mt genes into the pGEX vector (GE Healthcare), yielding pGEX-wt and pGEX-mt, respec-tively. We constructed pRL-EMCV-FL by replacing the fragment containing HCV IRES, fLuc gene and the 3 noncoding region of pRC22F (ref. 38) with that conta
162、ining EMCV IRES, fLuc gene and the 3-noncoding region of pT7- IRES-fLuc (ref. 39).In vitro RNA synthesis and RNA transfection. We generated capped and polyadenylated CAT RNA, nsp1 RNA, nsp1-mt RNA, m7G-FF RNA, rLuc RNA, Ren-HCV-FF RNA and Ren-CrPV-FF RNA by linearizing and transcrib-ing the plasmids
163、 pcD-CAT, pcD-Nsp1-wt, pcD-Nsp1-mt (ref. 10), pST-fluc, pRL-SV40 (Promega), pRC22F (ref. 38) and CrPV1.1, respectively, using an mMESSAGE mMACHINE T7 Ultra kit (Ambion). We synthesized capped and non-polyadenylated Ren-EMCV-FF RNA from pRL-EMCV-FL after linearization by ClaI digestion 288 nt upstrea
164、m of the 3 end of the fLuc gene. We used TransIT mRNA (Mirus) for transfecting in vitro RNA transcripts into subconfluent 293 cells.Expression and purification of recombinant nsp1 protein. We purified GST-fused proteins expressed in E. coli BL21-CodonPlus DE3 cells (Stratagene) using glutathione Sep
165、harose 4B affinity beads (GE Healthcare) followed by cleavage of GST from the GST-fused protein using PreScission protease (GE Healthcare).In vitro translation assay. We performed the translation reactions using the Retic Lysate IVT kit (Ambion). After preincubating RRL with nsp1, GST or nsp1-mt for
166、 10 min at 4 C, we added RNA and incubated the samples at 30 C in the pres-ence of 35Smethionine (1,000 Ci mmol1; MP Biomedicals). We resolved the translation products by 12% SDS-PAGE and visualized by autoradiography. We performed densitometric analysis using ImageJ software to quantify the bands.I
167、mmunoprecipitation of rRNA. At 8 h after transfection of 293 cells with RNAs, we prepared the cell lysates using a buffer containing 50 mM Tris-HCl (pH 7.5), 5 mM MgCl2, 100 mM KCl and 1% (v/v) Triton-X-100 and incubated the lysates with an anti-myc antibody (Upstate Biotech.) at 4 C. We collected t
168、he immune complexes after incubation with Protein G Plus-Agarose (Santa Cruz Biotech.) at 4 C followed by centrifugation. After washing the pellets three times in a buffer containing 10 mM HEPES (pH 7.4), 500 mM KCl, 2.5 mM MgCl2 and 1 mM DTT, we extracted RNA and performed northern blot analysis us
169、ing digoxigenin-labeled oligonucleotide probes40 to detect 18S and 28S rRNAs. We resolved the immunoprecipitated proteins in the pellets by SDS-PAGE and western blot analysis.Polysome analysis. At 8 h after transfection of 293 cells with RNAs, we prepared the cell lysates in a buffer containing 50 m
170、M Tris-HCl, pH 7.5, 5 mM MgCl2, 100 mM KCl, 1% (v/v) Triton-X-100, 2 mM DTT, 100 g ml1 CHX and 0.5 mg ml1 heparin, applied them onto a 10% to 50% sucrose gradient containing the same buffer and centrifuged at 39,000 r.p.m. in a Beckman SW41 rotor at 4 C for 2 h. After fractionating the gradient frac
171、tions using a gradient fractionator, we monitored the absorbance at 254 nm using an ISCO UA-6 spectrophotometer. We precipitated the proteins from each fraction using trichloroacetic acid and detected them by western blot analysis. We extracted rRNAs from the fractions and visualized them on a gel b
172、y staining with ethidium bromide.Ribosome binding assay. We performed the ribosome binding assay as described previously16. After preincubating RRL with GST, nsp-1 or nsp1-mt for 10 min at 4 C, we incubated the samples with -32PUTP-labeled, in vitro transcribed rLuc RNA in the presence of 0.6 mM CHX
173、 or 1 mM GMP-PNP for 10 min at 30 C. After centrifugation through 10% to 40% sucrose gradients (38,000 r.p.m. for 3.5 h in a Beckman SW41 rotor), we collected fractions from each gradient and determined the radioactivity in each fraction in a scintillation counter.Toe printing analysis. We performed
174、 toe printing analysis as described previously18,41. After pre-annealing the 5 32P-labeled primer DNA (5-TTTT TCTGAATCATAATAATTAA-3) (40,000 c.p.m.) to rLuc RNA by heating for 1 min at 65 C, followed by incubation at 37 C for 10 min, we preincubated RRL with 1 g of GST, nsp1, or nsp1-mt for 10 min a
175、t 4 C and then incu-bated the samples with 0.6 mM CHX or 0.6 mM CHX and 1 mM GMP-PNP for 5 min at 30 C. After incubation, we added the primermRNA complexes to the samples and incubated for an additional 10 min at 30 C. We diluted the reactions 20-fold with buffer containing 0.5 mM CHX, 7 mM magnesiu
176、m acetate, 100 mM potassium acetate, 0.5 mM dATP, dCTP, dGTP, and dTTP, 20 mM Tris-HCl, pH 7.4, 2 mM DTT and 1 unit l1 SuperScript III (Invitrogen), and incubated them at 30 C for 10 min. We resolved the extracted primer extension products on an 8% sequencing gel.Primer extension analysis. After pre
177、incubating RRL with GST, nsp-1, or nsp-1-mt for 10 min at 4 C, we incubated the samples with 0.6 mM CHX or 0.6 mM CHX and 1 mM GMP-PNP for 5 min at 30 C. Then, we added rLuc RNA to the samples and incubated them for 10 min at 30 C. After extracting total RNAs, we incubated the RNAs with the 5-end la
178、beled primer as described above and performed primer extension using a Primer Extension System (Promega). We resolved the extracted primer extension products on 8% sequencing gel.Assay for modification of radiolabeled in vitro-synthesized RNAs in RRL. We generated the cap-labeled rLuc RNA by incubat
179、ing uncapped rLuc RNA with vaccinia virus guanylyltransferase (Ambion) in the presence of -32PGTP (3,000 Ci mmol1; MP Biomedicals) at 37 C for 1 h. We gener-ated the 3-end labeled rLuc RNA by incubating rLuc RNA with T4 RNA ligase (Ambion) in the presence of 32PpCp (cytidine-3,5-bisphosphate; 3,000
180、Ci mmol1, MP Biomedicals) and ATP at 4 C for 16 h. We purified all labeled RNA transcripts with the RNeasy kit (Qiagen). We incubated the 32P-labeled RNA transcripts with nsp1, GST or nsp1-mt in RRL. After 15 min incubation at 30 C in the presence of CHX and GMP-PNP, we extracted the RNAs and resolv
181、ed them on 5% sequencing gel.38. Wang, T.H., Rijnbrand, R.C. & Lemon, S.M. Core protein-coding sequence, but not core protein, modulates the efficiency of cap-independent translation directed by the internal ribosome entry site of hepatitis C virus. J. Virol. 74, 1134711358 (2000).39. Ikegami, T., P
182、eters, C.J. & Makino, S. Rift valley fever virus nonstructural protein NSs promotes viral RNA replication and transcription in a minigenome system. J. Virol. 79, 56065615 (2005).40. Banerjee, S., An, S., Zhou, A., Silverman, R.H. & Makino, S. RNase L-independent specific 28S rRNA cleavage in murine
183、coronavirus-infected cells. J. Virol. 74, 87938802 (2000).41. Ostareck, D.H., Ostareck-Lederer, A., Shatsky, I.N. & Hentze, M.W. Lipoxygenase mRNA silencing in erythroid differentiation: The 3UTR regulatory complex controls 60S ribosomal subunit joining. Cell 104, 281290 (2001). 2009 Nature America, Inc. All rights reserved.
