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1、LDLD5050计算方法之计算方法之HornHorn氏法氏法原原 理理 HornHorn氏法，氏法，又叫平均移动法、剂量递增法，是利又叫平均移动法、剂量递增法，是利用剂量对数与死亡率用剂量对数与死亡率( (反应率反应率) )的转换数的转换数( (即几率单位即几率单位) )呈直线关系而设计的方法。呈直线关系而设计的方法。设设 计计 原原 则则 4 4个剂量组；个剂量组； 每组动物数相等；每组动物数相等； 每组每组4 4只或只或5 5只；只； 设计剂量时可根据化学物致死剂量范围的宽窄应设计剂量时可根据化学物致死剂量范围的宽窄应用两个剂量系列，其组距分别为用两个剂量系列，其组距分别为2.152.15
2、倍和倍和3.163.16倍。倍。 根据每组动物数、组距和每组动物死亡数，即可根据每组动物数、组距和每组动物死亡数，即可从从HornHorn氏表中查得氏表中查得LDLD5050及其及其9595可信限。可信限。优优 缺缺 点点 使用动物数少；使用动物数少； 可直接从可直接从HornHorn氏表查出氏表查出LDLD5050及其及其9595可信可信限，不需计算，甚为简便。限，不需计算，甚为简便。 但但LDLD5050的的9595可信区间范围较大，方法精可信区间范围较大，方法精确度尚不够。确度尚不够。HornHornHornHorn氏表氏表氏表氏表1-1 1-1 1-1 1-1 每组每组每组每组4 4
3、4 4只动物，组距只动物，组距只动物，组距只动物，组距3.163.163.163.16倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.3160.316 10 10t t剂量剂量1 11.001.00 10 10t t 剂量剂量2 21.001.00剂量剂量2 23.163.16 剂量剂量3 33.163.16剂量剂量3 310.010.0 剂量剂量4 410.010.0剂量剂量4 431.631.61 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限0 00 02 24 43.163.161.631.636.156.1510.
4、010.05.145.1419.419.40 00 03 34 42.372.371.331.334.224.227.507.504.224.2213.313.30 00 04 44 41.781.785.625.620 01 11 14 43.163.161.401.407.147.1410.010.04.434.4322.622.60 01 12 24 42.372.370.9840.9845.715.717.507.503.113.1118.118.10 01 13 34 41.781.780.7880.7884.014.015.625.622.492.4912.712.70 01 14
5、 44 41.331.330.7500.7502.372.374.224.222.372.377.507.500 02 22 24 41.781.780.6950.6954.554.555.625.622.202.2014.414.40 02 23 34 41.331.330.5540.5543.213.214.224.221.751.7510.210.20 02 24 44 41.001.000.5140.5141.941.943.163.161.631.636.156.150 03 33 34 41.001.000.4430.4432.262.263.163.161.401.407.147
6、.141 10 02 24 43.163.161.301.307.677.6710.010.04.124.1224.324.31 10 03 34 42.152.150.9590.9594.844.846.816.813.033.0315.315.31 10 04 44 41.471.470.8800.8802.452.454.644.642.782.787.747.741 11 11 14 43.163.161.071.079.369.3610.010.03.383.3829.629.61 11 12 24 42.152.150.6490.6497.157.156.816.812.052.0
7、522.622.61 11 13 34 41.471.470.4420.4424.874.874.644.641.401.4015.415.41 11 14 44 41.001.000.3380.3382.962.963.163.161.071.079.309.301 12 22 24 41.471.470.3790.3795.685.684.644.641.201.2018.018.01 12 23 34 41.001.000.2460.2464.064.063.163.160.7790.77912.812.82 20 02 24 43.163.160.8370.83711.911.910.
8、010.02.652.6537.837.82 20 03 34 41.781.780.4710.4716.726.725.625.621.491.4921.221.22 20 04 44 41.001.000.2650.2653.783.783.163.160.8370.83711.911.92 21 11 14 43.163.160.6210.62116.116.110.010.01.961.9650.950.92 21 12 24 41.781.780.2710.27111.711.75.625.620.8580.85836.936.92 21 13 34 41.001.000.1220.
9、1228.188.183.163.160.3860.38625.925.9HornHorn氏表氏表氏表氏表1-2 1-2 每组每组每组每组4 4只动物，组距只动物，组距只动物，组距只动物，组距3.163.16倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.3160.316 10 10t t剂量剂量1 11.001.00 10 10t t 剂量剂量2 21.001.00剂量剂量2 23.163.16 剂量剂量3 33.163.16剂量剂量3 310.010.0 剂量剂量4 410.010.0剂量剂量4 431.631.61 12 23 34 4LDLD5050可可
10、 信信 限限LDLD5050可可 信信 限限2 22 22 24 41.001.000.1000.10010.010.03.163.160.3160.31631.631.63 30 02 24 43.163.160.2210.22145.245.210.010.00.7000.7001431433 30 03 34 41.001.000.03850.038526.026.03.163.160.1220.12282.182.13 31 11 14 43.163.160.1220.12282.182.110.010.00.3850.385260.0260.03 31 12 24 41.001.00
11、0.01490.014966.966.93.163.160.04720.0472212.0212.00 00 03 33 33.163.161.071.079.369.3610.010.03.383.3829.629.60 00 04 43 32.152.151.291.293.593.596.816.814.084.0811.411.40 01 12 23 33.163.160.7790.77912.812.810.010.02.462.4640.640.60 01 13 33 32.152.150.6490.6497.157.156.816.812.052.0522.622.60 01 1
12、4 43 31.471.470.6540.6543.303.304.644.642.072.0710.410.42 22 22 23 32.152.150.5560.5568.348.346.816.811.761.7626.426.40 02 23 33 31.471.470.4420.4424.874.874.644.641.401.4015.415.40 02 24 43 31.001.000.4120.412 2.43 2.433.163.161.301.307.677.670 03 33 33 31.001.000.3380.3382.962.963.163.161.071.079.
13、369.361 10 03 33 33.163.160.6210.62116.116.110.010.01.961.9650.950.91 10 04 43 31.781.780.7780.7784.014.015.625.622.492.4912.712.71 11 12 23 33.163.160.3860.38625.925.910.010.01.221.2281.881.81 11 13 33 31.781.780.2880.28811.011.05.625.620.9110.91134.734.71 11 14 43 31.001.000.1960.1965.095.093.163.
14、160.6210.62116.116.11 12 22 23 31.781.780.2290.22913.813.85.625.620.7250.72543.643.61 12 23 33 31.001.000.1220.1228.188.183.163.160.3860.38625.925.92 20 03 33 33.163.160.1220.12282.182.110.010.00.3850.3852602602 20 04 43 31.001.000.07000.070014.314.33.163.160.2210.22145.245.22 21 12 23 33.163.160.04
15、720.047221221210.010.00.1490.1496696692 21 13 33 31.001.000.01490.014966.966.93.163.160.04720.04722122122 22 22 23 31.001.000.01000.01001001003.163.160.03160.0316316316HornHorn氏表氏表氏表氏表1-3 1-3 每组每组每组每组4 4只动物，组距只动物，组距只动物，组距只动物，组距3.163.16倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.3160.316 10 10t t剂量剂量1 11
16、.001.00 10 10t t 剂量剂量2 21.001.00剂量剂量2 23.163.16 剂量剂量3 33.163.16剂量剂量3 310.010.0 剂量剂量4 410.010.0剂量剂量4 431.631.61 1组组2 2组组3 3组组4 4组组LDLD5050可可 信信 限限LDLD5050可可 信信 限限0 00 04 42 23.163.160.8370.83711.911.910.010.02.652.6537.837.80 01 13 32 23.163.160.3860.38625.925.910.010.01.221.2281.881.80 01 14 42 21.7
17、81.780.4710.4716.726.725.625.621.491.4921.221.20 02 22 22 23.163.160.3160.31631.631.610.010.01.001.001001000 02 23 32 21.781.780.2710.27111.711.75.625.620.8580.85836.936.90 02 24 42 21.001.000.2650.2653.783.783.163.160.8370.83711.911.90 03 33 32 21.001.000.1960.196 5.09 5.093.163.160.6210.62116.116.
18、11 10 04 42 23.163.160.2210.22145.245.210.010.00.7000.7001431431 11 13 32 23.163.160.04720.047221221210.010.00.1490.1496696691 11 14 42 21.001.000.03850.038526.026.03.163.160.1220.12282.182.11 12 22 22 23.163.160.03160.031631631610.010.00.1000.100100010001 12 23 32 21.001.000.01490.014966.966.93.163
19、.160.04720.04722122120 02 23 31 13.163.160.04720.047221221210.010.00.1490.1496696690 02 24 41 11.001.000.07000.070014.314.33.163.160.2210.22145.245.20 03 33 31 11.001.000.03850.038526.026.03.163.160.1220.12282.182.10 01 14 41 13.163.160.1220.12282.182.110.010.00.3850.385260260HornHorn氏表氏表氏表氏表2-1 2-1
20、 每组每组每组每组4 4只动物，组距只动物，组距只动物，组距只动物，组距2.152.15倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.4640.464 10 10t t剂量剂量1 11.001.00 10 10t t剂量剂量2 22.152.151010t t 剂量剂量2 21.001.00剂量剂量2 22.152.15剂量剂量3 34.644.64 剂量剂量3 32.152.15剂量剂量3 34.644.64剂量剂量4 410.010.0 剂量剂量4 44.644.64剂量剂量4 410.010.0剂量剂量2 221.521.51 12 23 34 4LDL
21、D5050可可 信信 限限LDLD5050可可 信信 限限LDLD5050可可 信信 限限0 00 02 24 42.152.151.381.383.363.364.644.642.982.987.237.2310.010.06.426.4215.615.60 00 03 34 41.781.781.211.212.612.613.833.832.612.615.625.628.258.255.625.6212.112.10 00 04 44 41.471.473.163.166.816.810 01 11 14 42.152.151.251.253.713.714.644.642.702.7
22、07.997.9910.010.05.815.8117.217.20 01 12 24 41.781.780.9890.9893.203.203.833.832.132.136.896.898.258.254.594.5914.814.80 01 13 34 41.471.470.8530.8532.532.533.163.161.841.845.445.446.816.813.963.9611.711.70 01 14 44 41.211.210.8250.8251.781.782.612.611.781.783.833.835.625.623.833.838.258.250 02 22 2
23、4 41.471.470.7840.7842.752.753.163.161.691.695.925.926.816.813.643.6412.712.70 02 23 34 41.211.210.6740.6742.182.182.612.611.451.454.694.695.625.623.133.1310.110.10 02 24 44 41.001.000.6420.6421.561.562.152.151.381.383.363.364.644.642.982.987.237.230 03 33 34 41.001.000.5810.5811.721.722.152.151.251
24、.253.713.714.644.642.702.707.997.991 10 02 24 42.152.151.191.193.893.894.644.642.572.578.388.3810.010.05.545.5418.118.11 10 03 34 41.671.670.9730.9732.862.863.593.592.102.106.166.167.747.744.514.5113.313.31 10 04 44 41.291.290.9180.9181.821.822.782.781.981.983.913.915.995.994.264.268.438.431 11 11 1
25、4 42.152.151.041.044.444.444.644.642.252.259.579.5710.010.04.854.8520.620.61 11 12 24 41.671.670.7500.7503.713.713.593.591.611.618.008.007.747.743.843.8417.217.21 11 13 34 41.291.290.5800.5802.872.872.782.781.251.256.196.195.995.992.692.6913.313.31 11 14 44 41.001.000.4850.4852.062.062.152.151.041.0
26、44.444.444.644.642.252.259.579.571 12 22 24 41.291.290.5240.5243.183.182.782.781.131.136.866.865.995.992.432.4314.814.81 12 23 34 41.001.000.3930.3932.552.552.152.150.8460.8465.485.484.644.641.821.8211.811.82 20 02 24 42.152.150.8880.8885.235.234.644.641.911.9111.311.310.010.04.124.1224.324.32 20 03
27、 34 41.471.470.6050.6053.563.563.163.161.301.307.677.676.816.812.812.8116.516.52 20 04 44 41.001.000.4120.4122.432.432.152.150.8880.8885.235.234.644.641.911.9111.311.32 21 11 14 42.152.150.7280.7286.386.384.644.641.571.5713.713.710.010.03.383.3829.629.62 21 12 24 41.471.470.4190.4195.145.143.163.160
28、.9030.90311.111.16.816.811.951.9523.923.92 21 13 34 41.001.000.2460.2464.064.062.152.150.5310.5318.758.754.644.641.141.1418.818.8HornHorn氏表氏表氏表氏表2-2 2-2 每组每组每组每组4 4只动物，组距只动物，组距只动物，组距只动物，组距2.152.15倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.4640.464 10 10t t剂量剂量1 11.001.00 10 10t t剂量剂量2 22.152.15 10 10t
29、t 剂量剂量2 21.001.00剂量剂量2 22.152.15剂量剂量3 34.644.64 剂量剂量3 32.152.15剂量剂量3 34.644.64剂量剂量4 410.010.0 剂量剂量4 44.644.64剂量剂量4 410.010.0剂量剂量2 221.521.51 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限LDLD5050可可 信信 限限2 22 22 24 41.001.000.2150.2154.644.642.152.150.4640.46410.010.04.644.641.001.0021.521.53 30 02 24 42
30、.152.150.3660.36612.712.74.644.640.7890.78927.327.310.010.01.701.7058.958.93 30 03 34 41.001.000.1140.1148.778.772.152.150.2460.24618.918.94.644.640.5290.52940.740.73 31 11 14 42.152.150.2460.24618.918.94.644.640.5290.52940.740.710.010.01.141.1487.787.73 31 12 24 41.001.000.06070.060716.516.52.152.1
31、50.1310.13135.535.54.644.640.2820.28276.576.50 00 03 33 32.152.151.041.044.444.444.644.642.252.259.579.5710.010.04.854.8520.620.60 00 04 43 31.671.671.191.192.352.353.593.592.562.565.055.057.747.745.505.5010.910.90 01 12 23 32.152.150.8460.8465.485.484.644.641.821.8211.811.810.010.03.933.9325.525.50
32、 01 13 33 31.671.670.7500.7503.713.713.593.591.611.618.008.007.747.743.843.8417.217.20 01 14 43 31.291.290.7530.7532.212.212.782.781.621.624.774.775.995.993.503.5010.310.30 02 22 23 31.671.670.6760.6764.114.113.593.591.461.468.868.867.747.743.143.1419.119.10 02 23 33 31.291.290.5800.5802.872.872.782
33、.781.251.256.196.195.995.992.692.6913.313.30 02 24 43 31.001.000.5540.5541.811.812.152.151.191.193.893.894.644.642.572.578.388.380 03 33 33 31.001.000.4850.4852.062.062.152.151.041.044.444.444.644.642.252.259.579.571 10 03 33 32.152.150.7280.7286.386.384.644.641.571.5713.713.710.010.03.383.3829.629.
34、61 10 04 43 31.471.470.8530.8532.532.533.163.161.841.845.445.446.816.813.963.9611.711.71 11 12 23 32.152.150.5310.5318.758.754.644.641.141.1418.818.810.010.02.462.4640.640.61 11 13 33 31.471.470.4360.4364.944.943.163.160.9400.94010.610.66.816.812.022.0222.922.91 11 14 43 31.001.000.3380.3382.962.962
35、.152.150.7280.7286.386.384.644.641.571.5713.713.71 12 22 23 31.471.470.3750.3755.755.753.163.160.8070.80712.412.46.816.811.741.7426.726.71 12 23 33 31.001.000.2460.2464.064.062.152.150.5310.5318.758.754.644.641.141.1418.818.82 20 03 33 32.152.150.2460.24618.918.94.644.640.5290.52940.740.710.010.01.1
36、41.1487.787.72 20 04 43 31.001.000.1700.1705.895.892.152.150.3660.36612.712.74.644.640.7890.78927.327.32 21 12 23 32.152.150.1310.13135.535.54.644.640.2820.28276.576.510.010.00.6070.6071651652 21 13 33 31.001.000.06070.060716.516.52.152.150.1310.13135.535.54.644.640.2820.28276.576.52 22 22 23 31.001
37、.000.04640.046421.521.52.152.150.1000.10046.446.44.644.640.2150.215100100HornHornHornHorn氏表氏表氏表氏表2-3 2-3 2-3 2-3 每组每组每组每组4 4 4 4只动物，组距只动物，组距只动物，组距只动物，组距2.152.152.152.15倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.4640.464 10 10t t剂量剂量1 11.001.00 10 10t t剂量剂量2 22.152.15 10t 剂量剂量2 21.001.00剂量剂量2 22.152.15剂
38、量剂量3 34.644.64 剂量剂量3 32.152.15剂量剂量3 34.644.64剂量剂量4 410.010.0 剂量剂量4 44.644.64剂量剂量4 410.010.0剂量剂量2 221.521.51 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限LDLD5050可可 信信 限限0 00 04 42 22.152.150.8880.8885.235.234.644.641.911.9111.311.310.010.04.124.1224.324.30 01 13 32 22.152.150.5310.5318.758.754.644.641.
39、141.1418.818.810.010.02.462.4640.640.60 01 14 42 21.471.470.6050.6053.563.563.163.161.301.307.677.676.816.812.812.8116.516.50 02 22 22 22.152.150.4640.46410.010.04.644.641.001.0021.521.510.010.02.152.1546.446.40 02 23 32 21.471.470.4190.4195.145.143.163.160.9030.90311.111.16.816.811.951.9523.923.90
40、02 24 42 21.001.000.4120.4122.432.432.152.150.8880.8885.235.234.644.641.911.9111.311.30 03 33 32 21.001.000.3380.3382.962.962.152.150.7280.7286.386.384.644.641.571.5713.713.71 10 04 42 22.152.150.3660.36612.712.74.644.640.7890.78927.327.310.010.01.701.7058.958.91 11 13 32 22.152.150.1310.13135.535.5
41、4.644.640.2820.28276.576.510.010.00.6070.6071651651 11 14 42 21.001.000.1140.1148.778.772.152.150.2460.24618.918.94.644.640.5290.52940.740.71 12 22 22 22.152.150.1000.10046.446.44.644.640.2150.21510010010.010.00.4640.4642152151 12 23 32 21.001.000.06070.060716.516.52.152.150.1310.13135.535.54.644.64
42、0.2820.28276.576.50 02 23 31 12.152.150.1310.13135.535.54.644.640.2820.28276.576.510.010.00.6070.6071651650 02 24 41 11.001.000.1700.1705.895.892.152.150.3660.36612.712.74.644.640.7890.78927.327.30 03 33 31 11.001.000.1140.1148.778.772.152.150.2460.24618.918.94.644.640.5290.52940.740.70 01 14 41 12.
43、152.150.2460.24618.918.94.644.640.5290.52940.740.710.010.01.141.1487.787.7HornHorn氏表氏表氏表氏表3-1 3-1 每组每组每组每组5 5只动物，组距只动物，组距只动物，组距只动物，组距3.163.16倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.3160.3161010t t剂量剂量1 11.001.00 10 10t t 剂量剂量2 21.001.00剂量剂量2 23.163.16 剂量剂量3 33.163.16剂量剂量3 310.010.0 剂量剂量4 410.010.0剂量
44、剂量4 431.631.61 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限0 00 03 35 52.822.821.601.604.954.958.918.915.075.0715.715.70 00 04 45 52.242.241.411.413.553.557.087.084.474.4711.211.20 00 05 55 51.781.785.625.620 01 12 25 52.822.821.361.365.845.848.918.914.304.3018.518.50 01 13 35 52.242.241.081.084.644.6
45、47.087.083.423.4214.714.70 01 14 45 51.781.780.9270.9273.413.415.625.622.932.9310.810.80 01 15 55 51.411.410.8910.8912.242.244.474.472.822.827.087.080 02 22 25 52.242.241.011.014.974.977.087.083.193.1915.715.70 02 23 35 51.781.780.8010.8013.953.955.625.622.532.5312.512.50 02 24 45 51.411.410.6820.68
46、22.932.934.474.472.162.169.259.250 02 25 55 51.121.120.6380.6381.971.973.553.552.022.026.246.240 03 33 35 51.411.410.6360.6363.143.144.474.472.012.019.929.920 03 34 45 51.121.120.5420.5422.322.323.553.551.711.717.357.351 10 03 35 52.742.741.351.355.565.568.668.664.264.2617.617.61 10 04 45 52.052.051
47、.111.113.803.806.496.493.513.5112.012.01 10 05 55 51.541.541.071.072.212.214.874.873.403.406.986.981 11 12 25 52.742.741.101.106.826.828.668.663.483.4821.621.61 11 13 35 52.052.050.8060.8065.235.236.496.492.552.5516.516.51 11 14 45 51.541.540.6320.6323.753.754.874.872.002.0011.911.91 11 15 55 51.151
48、.150.5370.5372.482.483.653.651.701.707.857.851 12 22 25 52.052.050.7400.7405.705.706.496.492.342.3418.018.01 12 23 35 51.541.540.5340.5344.444.444.874.871.691.6914.114.11 12 24 45 51.151.150.4080.4083.273.273.653.651.291.2910.310.31 13 33 35 51.151.150.3780.3783.533.533.653.651.201.2011.211.22 20 03
49、 35 52.612.611.011.016.776.778.258.253.183.1821.421.42 20 04 45 51.781.780.7230.7234.374.375.625.622.292.2913.813.8HornHorn氏表氏表氏表氏表3-2 3-2 每组每组每组每组5 5只动物，组距只动物，组距只动物，组距只动物，组距3.163.16倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.3160.316 10 10t t剂量剂量1 11.001.00 10 10t t 剂量剂量2 21.001.00剂量剂量2 23.163.16 剂量剂量3
50、 33.163.16剂量剂量3 310.010.0 剂量剂量4 410.010.0剂量剂量4 431.631.61 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限2 20 05 55 51.211.210.5540.5542.652.653.833.831.751.758.398.392 21 12 25 52.612.610.7680.7688.878.878.258.252.432.4328.128.12 21 13 35 51.781.780.4840.4846.536.535.625.621.531.5320.720.72 21 14 45 51.
51、211.210.3180.3184.624.623.833.831.001.0014.614.62 22 22 25 51.781.780.4340.4347.287.285.625.621.371.3723.023.02 22 23 35 51.211.210.2590.2595.675.673.833.830.8190.81917.917.90 00 04 44 42.742.741.271.275.885.888.668.664.03 4.03 18.618.60 00 05 54 42.052.051.431.432.942.946.496.494.53 4.53 9.319.310
52、01 13 34 42.742.740.9680.9687.757.758.668.663.06 3.06 24.524.50 01 14 44 42.052.050.8430.8435.005.006.496.492.67 2.67 15.315.30 01 15 54 41.541.540.8330.8332.852.854.874.872.63 2.63 9.019.010 02 22 24 42.742.740.8960.8968.378.378.668.662.832.8326.526.50 02 23 34 42.052.050.7110.7115.935.936.496.492.
53、252.2518.718.70 02 24 44 41.541.540.6040.6043.923.924.874.871.911.9112.412.40 02 25 54 41.151.150.5680.5682.352.353.653.651.801.807.427.420 03 33 34 41.541.540.5550.5554.274.274.874.871.761.7613.513.50 03 34 44 41.151.150.4630.4632.882.883.653.651.471.479.109.101 10 04 44 42.612.610.9530.9537.157.15
54、8.258.253.013.0122.622.61 10 05 54 41.781.781.031.033.063.065.625.623.273.279.689.681 11 13 34 42.612.610.6580.65810.410.48.258.252.082.0832.732.71 11 14 44 41.781.780.5280.5285.985.985.625.621.671.6718.918.91 11 15 54 41.211.210.4420.4423.323.323.833.831.401.4010.510.51 12 22 24 42.612.610.5940.594
55、11.511.58.258.251.881.8836.336.31 12 23 34 41.781.780.4230.4237.487.485.625.621.341.3423.623.61 12 24 44 41.211.210.3050.3054.804.803.833.830.9660.96615.215.21 13 33 34 41.211.210.2760.2765.335.333.833.830.8710.87116.816.8HornHorn氏表氏表氏表氏表3-3 3-3 每组每组每组每组5 5只动物，组距只动物，组距只动物，组距只动物，组距3.163.16倍倍倍倍各剂量组动物死
56、亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.3160.316 10 10t t剂量剂量1 11.001.00 10 10t t 剂量剂量2 21.001.00剂量剂量2 23.163.16 剂量剂量3 33.163.16剂量剂量3 310.010.0 剂量剂量4 410.010.0剂量剂量4 431.631.61 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限2 20 04 44 42.372.370.5390.53910.410.47.507.501.701.7033.033.02 20 05 54 41.331.330.4460.4463
57、.993.994.224.221.411.4112.612.62 21 13 34 40.37 ?0.37 ?0.3070.30718.318.37.507.500.9700.97058.058.02 21 14 44 41.331.330.1870.1879.499.494.224.220.5920.59230.030.02 22 22 24 42.372.370.2620.26221.421.47.507.500.8300.83067.867.82 22 23 34 41.331.330.1370.13713.013.04.224.220.4330.43341.041.00 00 05 5
58、3 32.612.611.191.195.715.718.258.253.773.7718.118.10 01 14 43 32.612.610.6840.6849.959.958.258.252.162.1631.531.50 01 15 53 31.781.780.7230.7234.374.375.625.622.292.2913.813.80 02 23 33 32.612.610.5580.55812.212.28.258.251.761.7638.638.60 02 24 43 31.781.780.4840.4846.536.535.625.621.531.5320.720.70
59、 02 25 53 31.211.210.4670.4673.143.143.833.831.481.489.949.940 03 33 33 31.781.780.4340.4347.287.285.625.621.371.3723.023.00 03 34 43 31.211.210.3560.3564.124.123.833.831.131.1313.013.01 10 05 53 32.372.370.7930.7937.107.107.507.502.512.5122.422.41 11 14 43 32.372.370.3330.33316.916.97.507.501.051.0
60、553.453.41 11 15 53 31.331.330.3030.3035.875.874.224.220.9580.95818.618.61 12 23 33 32.372.370.2440.24423.123.17.507.500.7710.77173.073.01 12 24 43 31.331.330.1720.17210.310.34.224.220.5450.54532.632.61 13 33 33 31.331.330.1480.14812.112.14.224.220.4670.46738.138.1HornHorn氏表氏表氏表氏表4-1 4-1 每组每组每组每组5 5
61、只动物，组距只动物，组距只动物，组距只动物，组距2.152.15倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.4640.464 10 10t t剂量剂量1 11.001.00 10 10t t剂量剂量2 22.152.15 10 10t t 剂量剂量2 21.001.00剂量剂量2 22.152.15剂量剂量3 34.644.64 剂量剂量3 32.152.15剂量剂量3 34.644.64剂量剂量4 410.010.0 剂量剂量4 44.644.64剂量剂量4 410.010.0剂量剂量2 221.521.51 12 23 34 4LDLD5050可可 信信
62、 限限LDLD5050可可 信信 限限LDLD5050可可 信信 限限0 00 03 35 52.002.001.371.372.912.914.304.302.952.956.266.269.269.266.366.3613.513.50 00 04 45 51.711.711.261.262.332.333.693.692.712.715.015.017.947.945.845.8410.810.80 00 05 55 51.471.473.163.166.816.810 01 12 25 52.002.001.231.233.243.244.304.302.652.656.986.989
63、.269.265.705.7015.015.00 01 13 35 51.711.711.051.052.782.783.693.692.272.275.995.997.947.944.894.8912.912.90 01 14 45 51.471.470.9510.9512.272.273.163.162.052.054.884.886.816.814.414.4110.510.50 01 15 55 51.261.260.9260.9261.711.712.712.712.002.003.693.695.845.844.304.307.947.940 02 22 25 51.711.711
64、.011.012.912.913.693.692.172.176.286.287.947.944.674.6713.513.50 02 23 35 51.471.470.8620.8622.502.503.163.161.861.865.385.386.816.814.004.0011.611.60 02 24 45 51.261.260.7750.7752.052.052.712.711.671.674.414.415.845.843.603.609.509.500 02 25 55 51.081.080.7410.7411.571.572.332.331.601.603.393.395.0
65、15.013.443.447.307.300 03 33 35 51.261.260.7400.7402.142.142.712.711.591.594.624.625.845.843.433.439.959.950 03 34 45 51.081.080.6650.6651.751.752.332.331.431.433.783.785.015.013.083.088.148.141 10 03 35 51.961.961.221.223.143.144.224.222.632.636.766.769.099.095.665.6614.614.61 10 04 45 51.621.621.0
66、71.072.432.433.483.482.312.315.245.247.507.504.984.9811.311.31 10 05 55 51.331.331.051.051.701.702.872.872.262.263.653.656.196.194.874.877.877.871 11 12 25 51.961.961.061.063.603.604.224.222.292.297.757.759.099.094.944.9416.716.71 11 13 35 51.621.620.8660.8663.013.013.483.481.871.876.496.497.507.504
67、.024.0214.014.01 11 14 45 51.331.330.7370.7372.412.412.872.871.591.595.205.206.196.193.423.4211.211.21 11 15 55 51.101.100.6610.6611.831.832.372.371.421.423.953.955.115.113.073.078.518.511 12 22 25 51.621.620.8180.8183.193.193.483.481.761.766.876.877.507.503.803.8014.814.81 12 23 35 51.331.330.6580.
68、6582.702.702.872.871.421.425.825.826.196.193.053.0512.512.51 12 24 45 51.101.100.5500.5502.202.202.372.371.191.194.744.745.115.112.552.5510.210.21 13 33 35 51.101.100.5320.5322.322.322.372.371.131.134.994.995.115.112.432.4310.810.82 20 03 35 51.901.901.001.003.583.584.084.082.162.167.717.718.80.8.80
69、.4.664.6616.616.62 20 04 45 51.471.470.8060.8062.672.673.163.161.741.745.765.766.816.813.743.7412.412.4HornHorn氏表氏表氏表氏表4-2 4-2 每组每组每组每组5 5只动物，组距只动物，组距只动物，组距只动物，组距2.152.15倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只) ) 剂量剂量1 10.4640.464 10 10t t剂量剂量1 11.001.00 10 10t t剂量剂量2 22.152.15 10 10t t 剂量剂量2 21.001.00剂量剂量2 22.
70、152.15剂量剂量3 34.644.64 剂量剂量3 32.152.15剂量剂量3 34.644.64剂量剂量4 410.010.0 剂量剂量4 44.644.64剂量剂量4 410.010.0剂量剂量2 221.521.51 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限LDLD5050可可 信信 限限2 20 05 55 51.141.140.6740.6741.921.922.452.451.451.454.134.135.285.283.133.138.898.892 21 12 25 51.901.900.8390.8394.294.294.0
71、84.081.811.819.239.238.808.803.893.8919.919.92 21 13 35 51.471.470.6160.6163.503.503.163.161.331.337.537.536.816.812.862.8616.216.22 21 14 45 51.141.140.4660.4662.772.772.452.451.001.005.985.985.285.282.162.1612.912.92 22 22 25 51.471.470.5730.5733.763.763.163.161.241.248.108.106.816.812.662.6617.41
72、7.42 22 23 35 51.141.140.4060.4063.183.182.452.450.8750.8756.856.855.285.281.891.8914.814.80 00 04 44 41.961.961.181.183.263.264.224.222.532.537.027.029.099.095.465.4615.115.10 00 05 54 41.621.621.271.272.052.053.483.482.742.744.424.427.507.505.905.909.539.530 01 13 34 41.961.960.9780.9783.923.924.2
73、24.222.112.118.448.449.099.094.544.5418.218.20 01 14 44 41.621.620.8930.8932.922.923.483.481.921.926.306.307.507.504.144.1413.613.60 01 15 54 41.331.330.8850.8852.012.012.872.871.911.914.334.336.196.194.114.119.339.330 02 22 24 41.961.960.9300.9304.124.124.224.222.002.008.888.889.099.094.314.3119.11
74、9.10 02 23 34 41.621.620.7970.7973.283.283.483.481.721.727.067.067.507.5015.215.20 02 24 44 41.331.330.7150.7152.402.402.872.871.541.545.365.366.196.1911.511.50 02 25 54 41.101.100.6860.6861.771.772.372.371.481.483.803.805.115.118.198.190 03 33 34 41.331.330.6760.6762.632.632.872.871.461.465.675.676
75、.196.1912.212.20 03 34 44 41.101.100.5990.5992.022.022.372.371.291.294.364.365.115.119.399.391 10 04 44 41.901.900.9690.9693.713.714.084.082.092.097.997.998.808.8017.217.21 10 05 54 41.471.471.021.022.112.113.163.162.202.204.544.546.816.819.789.781 11 13 34 41.901.900.7570.7574.754.754.084.081.631.6
76、310.210.28.808.8022.022.01 11 14 44 41.471.470.6540.6543.303.303.163.161.411.417.107.106.816.8115.315.31 11 15 54 41.141.140.5810.5812.222.222.452.451.251.254.794.795.285.2810.310.31 12 22 24 41.901.900.7060.7065.095.094.084.081.521.5211.011.08.808.8023.623.61 12 23 34 41.471.470.5640.5643.823.823.1
77、63.161.211.218.248.246.816.8117.717.71 12 24 44 41.141.140.4540.4542.852.852.452.450.9770.9776.136.135.285.2813.213.21 13 33 34 41.141.140.4230.4233.053.052.452.450.9120.9126.576.575.285.281.971.9714.214.2HornHorn氏表氏表氏表氏表4-3 4-3 每组每组每组每组5 5只动物，组距只动物，组距只动物，组距只动物，组距2.152.15倍倍倍倍各剂量组动物死亡数各剂量组动物死亡数( (只只)
78、 ) 剂量剂量1 10.4640.464 10 10t t剂量剂量1 11.001.00 10 10t t剂量剂量2 22.152.15 10 10t t 剂量剂量2 21.001.00剂量剂量2 22.152.15剂量剂量3 34.644.64 剂量剂量3 32.152.15剂量剂量3 34.644.64剂量剂量4 410.010.0 剂量剂量4 44.644.64剂量剂量4 410.010.0剂量剂量2 221.521.51 12 23 34 4LDLD5050可可 信信 限限LDLD5050可可 信信 限限LDLD5050可可 信信 限限2 20 04 44 41.781.780.662
79、0.6624.784.783.833.831.431.4310.310.38.258.253.073.0722.222.22 20 05 54 41.211.210.5830.5832.522.522.612.611.261.265.425.425.625.622.712.7111.711.72 21 13 34 41.781.780.4550.4556.956.953.833.830.9800.98015.015.08.258.252.112.1132.332.32 21 14 44 41.211.210.3270.3274.484.482.612.610.7050.7059.669.665
80、.625.621.521.5220.820.82 22 22 24 41.781.780.4100.4107.727.723.833.830.8830.88316.616.68.258.251.901.9035.835.82 22 23 34 41.211.210.2660.2665.525.522.612.610.5730.57311.911.95.625.621.231.2325.625.60 00 05 53 31.901.901.121.123.203.204.084.082.422.426.896.898.808.805.225.2214.814.80 01 14 43 31.901
81、.900.7770.7774.634.634.084.081.671.679.979.978.808.803.603.6021.521.50 01 15 53 31.471.470.8060.8062.672.673.163.161.741.745.765.766.816.813.743.7412.412.40 02 23 33 31.901.900.6730.6735.305.304.084.081.461.4611.411.48.808.803.153.1524.624.60 02 24 43 31.471.470.6160.6163.503.503.163.161.331.337.537
82、.536.816.812.862.8616.216.20 02 25 53 31.141.140.6020.6022.152.152.452.451.301.304.624.625.285.282.792.799.969.960 03 33 33 31.471.470.5730.5733.763.763.163.161.241.248.108.106.816.812.662.6617.417.40 03 34 43 31.141.140.5030.5032.572.572.452.451.081.085.545.545.285.282.332.3311.911.91 10 05 53 31.7
83、81.780.8560.8563.693.693.833.831.851.857.967.968.258.253.983.9817.117.11 11 14 43 31.781.780.4810.4816.586.583.833.831.041.0414.214.28.258.252.232.2330.530.51 11 15 53 31.211.210.4510.4513.253.252.612.610.9720.9727.017.015.625.622.092.0915.115.11 12 23 33 31.781.780.3900.3908.118.113.833.830.8400.84017.517.58.258.251.811.8137.637.61 12 24 43 31.211.210.3100.3104.744.742.612.610.6680.66810.210.25.625.621.441.4422.022.01 13 33 33 31.211.210.2790.2795.265.262.612.610.6020.60211.311.35.625.621.301.3024.424.4
