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1、PH3-10缓冲溶液的配制方法1.磷酸氢二钠-柠檬酸缓冲液pH0.2 mol/LNa2HPO4（毫升）0.1mol/L柠檬酸 （毫升）pH0.2mol/LNa2HPO4（毫升）0.1 mol/L柠檬酸 （毫升）2.20.4010.605.210.729.282.41.2418.765.411.158.852.62.1817.825.611.608.402.83.1716.835.812.097.913.04.1115.896.012.637.373.24.9415.066.213.226.783.45.7014.306.413.856.153.66.4413.566.614.555.453.8
2、7.1012.906.815.454.554.07.7112.297.016.473.534.28.2811.727.217.392.614.48.8211.187.418.171.834.69.3510.657.618.731.274.89.8610.147.819.150.855.010.309.708.019.450.55Na2HPO4分子量 =14.98 , 0.2 mol/L 溶液为 28.40 克/升。Na2HPO4 2H2O 分子量=178.05，0.2 mol/L 溶液含 35.01 克/升。C4H2O7 H2O 分子量 =210.14, 0.1 mol/L 溶液为 21.01
3、 克/升。2 柠檬酸-氢氧化钠-盐酸缓冲液pH钠离子浓度 （mol/L）柠檬酸（克）C6H 8O7 H 2O氢氧化钠（克）NaOH 97%盐酸（毫升）HCl （浓）最终体积（刑2.20.2021084160103.10.2021083116103.30.2021083106104.30.202108345105.30.3524514468105.80.45285186105106.50.3826615612610 使用时可以每升中加入 1克克酚，若最后pH值有变化，再用少量50%氢氧化钠溶液或浓盐酸调节，冰箱保存。3.甘氨酸-盐酸缓冲液（0.05mol/L）X毫升0.2 mol/L甘氨酸+Y毫
4、升0.2 mol/L HCI，再加水稀释至 200毫升pHXYpHXY2.05044.03.05011.42.45032.43.2508.22.65024.23.4506.42.85016.83.6505.0甘氨酸分子量 =75.07 , 0.2 mol/L甘氨酸溶液含15.01克/升。X毫升0.2 mol/L邻苯二甲酸氢钾 + 0.2 mol/L HCI，再加水稀释到 20毫升pH(20 C )XYpH(20 C)XY2.254.0703.251.4702.453.9603.450.9902.653.2953.650.5972.852.6423.850.2633.052.022邻苯二甲酸氢钾
5、分子量=204.23，0.2 mol/L邻苯二甲酸氢溶液含40.85克/升5 柠檬酸-柠檬酸钠缓冲液（0.1 mol/L ）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）pH0.1 mol/L柠檬酸（毫升）0.1 mol/L柠檬酸钠（毫升）3.018.61.45.08.211.83.217.22.85.27.312.73.416.04.05.46.413.63.614.95.15.65.514.53.814.06.05.84.715.34.013.16.96.03.816.24.212.37.76.22.817.24.411.48.66.42.018.04.610.39
6、.76.61.418.64.89.210.8柠檬酸C6H8O7 H2O:分子量210.14, 0.1 mol/L溶液为21.01克/升。柠檬酸钠 Na3 C6H5O7 2H2O:分子量 294.12, 0.1 mol/L 溶液为 29.41 克/升。 柠檬酸 C6H8O7 H2O: 2.1g柠檬酸钠 Na3 C6H5O7 2H2O: 2.94gH2O: 100 ml6 .乙酸-乙酸钠缓冲液（0.2 mol/L ）pH(18 C )0.2 mol/LNaAc(毫升)0.3 mol/LHAc(毫升)pH(18 C)0.2 mol/LNaAc(毫升)0.3 mol/LHAc(毫升)2.60.759.
7、254.85.904.103.81.208.805.07.003.004.01.808.205.27.902.104.22.657.355.48.601.404.43.706.305.69.100.904.64.905.105.89.400.60Na2Ac 3H2O 分子量 =136.09, 0.2 mol/L 溶液为 27.22 克/升。7磷酸盐缓冲液（1）磷酸氢二钠-磷酸二氢钠缓冲液（0.2）pH0.2 mol/L0.3 mol/LpH0.2 mol/L0.3 mol/LNa2HPO4NaH 2PO4Na2HPO4NaH2PO4（毫升）（毫升）（毫升）（毫升）5.88.092.07.061
8、.039.05.910.090.07.167.033.06.012.387.77.272.028.06.115.085.07.377.023.06.218.581.57.481.019.06.322.577.57.584.016.06.426.573.57.687.013.06.531.568.57.789.510.56.637.562.57.891.58.56.743.556.57.993.07.06.849.551.08.094.75.36.955.045.0Na2HPO4 2H2O 分子量 =178.05, 0.2 mol/L 溶液为 85.61 克/升。Na2HPO4 12H2O 分子
9、量 =358.22 , 0.2 mol/L 溶液为 71.64 克/升。Na2HPO4 2H2O 分子量 =156.03, 0.2 mol/L 溶液为 31.21 克/升。（2）磷酸氢二钠-磷酸二氢钾缓冲液（1/15 mol/L ）pHmol/L/15Na 2HPO4（毫升）mol/L/15KH 2PO4（毫升）pHmol/L/15Na 2HPO4（毫升）mol/L/15KH 2PO4（毫升）4.920.109.907.177.003.005.290.509.507.388.002.005.911.009.007.739.001.006.242.008.008.049.500.506.473.
10、007.008.349.750.256.644.006.008.679.900.106.815.005.008.1810.0006.986.004.00Na2HPO4 2H2O 分子量 =178.05, 1/15M 溶液为 11.876 克/升。KH 2PO4 分子量 =136.09, 1/15M 溶液为 9.078 克/升。&磷酸二氢钾-氢氧化钠缓冲液（0.05M）X毫升0.2M K 2PO4 + Y毫升0.2N NaOH加水稀释至29毫升pH（20 C）X （毫升）Y （毫升）pH（20 C）X （毫升）Y （毫升）5.850.3727.052.9636.050.5707.253.5006
11、.250.8607.453.9506.451.2607.654.2806.651.7807.854.5206.852.3658.054.6809.巴比妥钠-盐酸缓冲液（18C）pH0.04M巴比妥钠溶液0.2V盐酸pH0.04M 巴比 妥钠溶液（毫 升）0.2N盐酸（毫 升）6.810018.48.41005.217.010017.88.61003.827.210016.78.81002.527.410015.39.01001.657.610013.49.21001.137.810011.479.41000.708.01009.399.61000.358.21007.21100巴比妥钠盐分子量
12、=206.18;0.04M溶液为8.25克/升10. Tris -盐酸缓冲液（0.05M , 25C）50毫升0.1M三羟甲基氨基甲烷（Tris）溶液与X毫升0.1N盐酸混匀后，加水稀释至100毫升。pHX（毫升）pHX（毫升）7.1045.78.1026.27.2044.78.2022.97.3043.48.3019.97.4042.08.4017.27.5040.38.5014.77.6038.58.6012.47.7036.68.7010.37.8034.58.808.57.9032.08.907.08.0029.2三羟甲基氨基甲烷（Tris）HOCH2 CH2OHCHOCH2 NH2分子量=121.14;
